Antigens synthetic

Sela, M., Synthetic antigens and recent progi ss in immunology, in Peptides. Polypeptides and Proteins. Proceedings of the Rehovot Symposium on Poly (Amino Acids), Polypeptides and Proteins, John Wiley and sons, New York, 1974, pp. 495-509... 

It is widely believed that optimal methods for immunization against certain synthetic antigens may require the use of adjuvants, and this belief has stimulated a considerable amount of research aimed at developing new or improved adjuvants. The... 

Relate any testing to the clinical circumstances of probable use. Thus, if a new synthetic antigen or engineered antigen is selected and is intended for testing,... 

Table I. Specificity of serum antibodies obtained by Immunization with free synthetic antigenic sites of Mb...

These results were the first demonstration that Immunization with a small free (I.e. not coupled to any carrier) synthetic antigenic site Is effective In eliciting antibodies capable of binding to the ative protein molecule (48). 

Our finding that the small (6-7 residues) synthetic antigenic sites of Mb stimulated T-cells from peritoneal exudates of Mb-prlmed mice (47), suggested to us (73) that free synthetic peptides may be used during in vitro passage to enrich for T-cells with a selected specificity. Rowever, lymph node cells usually... 

Uhde, K., Fischer, R., and Commandeur, U. (2005). Expression of multiple foreign epitopes presented as synthetic antigens on the surface of potato virus X particles. Arc/t. Virol. 150 327-340. 

The specificity of the antiserum raised against the Lea neoglycoprotein (9) was determined by hapten-inhibition studies using synthetic oligosaccharides,72 and derivatives of bovine and horse serum-albumin to which the Lea trisaccharide had been attached.73 On the basis of hapten inhibition of precipitation, this antiserum was shown to be highly specific for the carbohydrate portion of the synthetic antigen,... 

Fig. 1.—Hapten Inhibition of the Precipitation of the Synthetic Antigen 8-Carboxy-octyl 4-Chloro-4-deoxy-0-D-galactopyranoside-BSA by Rabbit Antiserum Raised Against 8-Carboxyoctyl 0-D-Galactopyranoside-BSA (12 in Table I). [The haptens were the oj-earboxyalkyl /3-D-galactopyranosides having the aglycons (1) 8-carboxyoctyl, (2) 4-carboxybutyl, (3) 2-carboxyethyl, and (4) carboxymethyl (taken from Ref. 73).]...

R. A. Lerner, Chemical reactivity at an antibody binding site elidted by mechanistic design of a synthetic antigen, Proc. Natl. Acad. Sci. USA 1986, 83, 6736-6740. 

N. K. Kochetkov, Synthesis of fragments of bacterial polysaccharides and their application for preparation of synthetic antigens, Pure Appl. Chem., 56 (1984) 923-938. 

N. K. Kochetkov, B. A. Dmitriev, A. Y. Chernyak, and A. B. Levinsky, A new type of carbohydrate-containing synthetic antigen Synthesis of carbohydrate-containing polysaccharide copolymers with the specificity of 0 3 and 0 4 factors of Salmonella, Carbohydr. Res, 110 (1982) cl6-c20. 

A biomolecule with specificity for a special molecule (which is used as a ligamd on an immobilization matrix) is modified by an affinity tag. The tag may be a synthetic antigen, such as Flag, which consists of eight amino acids and binds to an immobilized monoclonal antibody (72). The interaction of Flag with the monoclonal antibody is Ca -dependent and reversible at low pH. These features constitute an undesirable constraint when applying this fusion to affinity immobilize some enzymes. 

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