Enhanced green fluorescent protein

Zeilhofer, H. U., Studler, B Arabadzisz, D. et al. (2005). Glycinergic neurons expressing enhanced green fluorescent protein in bacterial artificial chromosome transgenic mice. J. Comp. Neurol. 482, 123-41. 

Sinnecker D, Voigt P, Hellwig N, Schaefer M (2005) Reversible photobleaching of enhanced green fluorescent proteins. Biochemistry 44 7085-7094... 

Machova Z, Muhle C, Krauss U, Trehin R, Koch A, Merkle HP, Beck-Sickinger AG (2002) Cellular internalization of enhanced green fluorescent protein ligated to a human calcitonin-based carrier peptide. Chem Biochem 3 672-677. 

Woollhead AM, Baines DL (2006) Forskolin-induced cell shrinkage and apical translocation of functional enhanced green fluorescent protein-human alphaENaC in H441 lung epithelial cell monolayers. J Biol Chem 281 (8) 5158—5168... 

RhoB [also enhanced green fluorescent protein (EGFP)-RhoB, see above]... 

Kawamoto, S., Niwa, H., Tashiro, F., Sano, S., Kondoh, G., Takeda, J. et al. (2000) A novel reporter mouse strain that expresses enhanced green fluorescent protein upon Cre-mediated recombination. FEBS Lett., 470, 263-268. 

DOTMA E. coli E EBV ECFP ECV EGFP ELISA EYFP FACS FdG FH2 FH4 FK506 FLP propane-aminium-trifluoracetate 7V-[2,3-(dioleyloxy) propyl]-/V,/V,/V-trimethyl ammonium chloride Escherichia coli erythromycine operon/repressor Epstein-Barr virus enhanced cyan fluorescence protein extracellular viral particles enhanced green fluorescence protein enzyme-linked immunosorbent assay enhanced yellow fluorescence protein fluorescence-activated cell sorter fluorescein di- 3-D-galactopyranoside dihydrofolate tetrahydrofolate human immunophilins native recombinase isolated from the 2pm plasmid from Saccharomyces cerevisiae... 

Also, the Phe-64 -> Leu and Ser-65 —> Thr mutant shows higher fluorescence parameters than that of the wild GFP. Excitation at 458 nm yields a fluorescence spectrum with two peaks at 512 and 530 nm. The fluorescence properties of this enhanced green fluorescent protein (EGFP) were found to be similar to the recombinant glutathione S-transferase-EGFP (GST-EGFP) protein, expressed in Escherichia coli (Cinelli etal. 2004). 

Cinelli, R.A.G., Ferrari, A., Pellegrini, V., Tyagi, M., Giacca, M. and Beltram, F. (2004). The enhanced green fluorescent protein as a tool for the analysis of protein dynamics and localization Local fluorescence study at the single-molecule level. Photochemistry and Photobiology, 71, 771-776. 

Different-sized plasmid DNA may be used to create these polymer/DNA particles. For easy visual inspection of gene expression and quantitative cell population information enhanced green fluorescent protein (EGFP) DNA is often used. For high-throughput screening applications, luciferase (Luc) DNA is often used instead. 

Fig. 5. Electronic spectra of a mixture of ferric cytochrome c and enhanced green fluorescent protein EGFP before (—) and after (—) 490 nm photolysis indicating the formation of the ferrous form of heme c (6) with maxima at 413, 520, and 550 nm. Adapted from Ref (37).

Regional lymph nodes were removed 36 h after injection. The flow cytometric analysis of the crude lymph node cell suspension showed that 1.2% of the cells expressed the enhanced green fluorescent protein (EGFP) in comparison to cells from untreated controls (Fig. 4a). 

EGFP (biotinylated) Biotinylated Enhanced green fluorescent protein Panning in solution followed by capture on streptavidin-magnetic beads... 

EGFP Enhanced green fluorescent protein Panning on plastic-immobilized protein... 

Another much used yeast-based assay is the yEGFP assay (first described by Bovee et al., 2004). In this assay recombinant yeast cells are constructed that express the human estrogen reporter a (ER a) and yeast enhanced green fluorescence protein), the estrogenic potential is then measured by a Fluorometer. 

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