Cervical cancer HeLa cells

Finally, we wish to report the results for simulated cell smears. These experiments are being carried out to determine the best parameters for data acquisition, and to establish the variations of the cellular spectra from a homogeneous cell culture. Similar efforts have been undertaken before, where the cells were spin-deposited onto the microscope slides. However, for cultured cervical cancer (HeLa) cells, the resulting samples contained many cells that maintained their morphology in suspension quite well, and produced dried cells that were nearly spherical. Once dried, these cells could not be stained easily, and thus, their divisional activity could not be established after IR data acquisition. Furthermore, large spherical cells often gave spectral artifacts that made interpretation impossible.16... 

The transfection activity of the optimized R8-MEND was compared with Ade-noviras (see also Part I, Chapter 6), one of the strongest viral vectors, using human cervical cancer HeLa cells and a human lung epithelial carcinoma ceU hne A549 (Fig. 8.3). These ceUs display receptors for adenovirus serotype 5 such as CAR and in-... 

The effect of resveratrol on the expressions of matrix metalloproteinase and tissue inhibitors of metalloproteinase in cervical cancer HeLa cells was investigated [78]. The effect of resveratrol on the invasive ability of HeLa cells was observed with a transwell cell culture chamber. Activities of MM P-2 and MMP-9 were analyzed using gelatin zymog. Activities of TIMP-1 and TIMP-2 were analyzed using reverse zymog. mRNA expressions of MMP-2, MMP-9, TIMP-1, and TIMP-2 in HeLa cells were detected by RT-PCR. Their protein expressions were detected by Western blotting. Activities of MMP-2 and MMP-9 were found to be markedly... 

M. Miljkovic, M. Romeo, C. Matthaus and M. Diem, Infrared microspectroscopy of individual human cervical cancer (HeLa) cells suspended in growth medium. Biopolymers, 2004, 74, 172-175. 

Suresh D, Balakrishna MS, Rathinasamy K et al (2008) Large-bite bis(phosphite) ligand containing mesocyclic thioether moieties synthesis, reactivity, group 11 (Cu-I, Au-I) metal complexes and anticancer activity studies on a human cervical cancer (HeLa) cell line. Dalton Trans 2285-2292... 

In the laboratory the immortal cell lines are those derived from cancer. The most famous is the HeLa cancer cells that many of us use as one of the cell lines tested against various anticancer agents. The name HeLa cells is derived from Henrietta Lacks, a black woman, who died from cervical cancer. The cells are so strong that they invade other cell lines, both healthy and other cancer cell lines, giving contaminated or mutated cell lines. HeLa cells have good telomerase levels. If antisense RNA is added to HeLa cells so that the RNA contains the opposite message to the ordinary RNA in the telomerase, the effect is that the telomerase is blocked and the HeLa cells are no longer immortal and die after about 25 replications. 

FIGURE 8.8 A section of a single HeLa (human cervical cancer) cell stained with Phalloidin 350 (actin filaments) and MitoTracker488 (mitochondria) acquired using selective two-photon excitation, without fluorescence color filters. The image size is about 25 (xm. (From Dantus, M., Lozovoy, V. V., and Pastirk, I. Laser Focus World, 43(5) 101-104. 2007. Used with permission.)... 

Henrietta Lacks (HeLa cells immortal cell line derived from H.L. s cervical cancer cells) heteronuclear correlation (NMR) hydrophobin... 

EAG has a rather restricted distribution in healthy tissues it expresses nearly exclusively in brain, slightly in placenta and transiently in skeletal muscle (Occhiodoro et al. 1998 Pardo et al. 1999). In contrast to its restricted distribution in normal tissues, expression of EAG has been found in several human somatic cancer cell lines including HeLa cervix carcinoma, MCF-7 and SHSY-5Y neuroblastoma derived from breast tumor, melanoma IGR1, rhabdomyosarcoma, etc. (Meyer 1998, 1999 Crociani 2003). Most notably, EAG mRNA expression was found in 100% of the human cervical cancer sam-... 

Butter yellow is active in inducing unscheduled DNA synthesis in Hela human cervical cancer cells. It was mutagenic in Salmonella typhimurium TAIOO and TA98. 

In Vitro. Mouse connective tissue (L cells), human cervical carcinoma (HeLa), and human amnion (WISH) cancer cells were used to determine the toxic and nontoxic levels of several of the... 

Ehrlich tumours are poorly differentiated malignant tumours (originally a breast carcinoma in a mouse), which grow in solid and ascetic forms. HeLa cells are a cell line developed from cervical cancer of a woman ( enriette Lacks), who died from that cancer in 1951. 

A HeLa cell (also Hela or hela cell) is an immortal cell line used in medical research. The cell Une was derived from cervical cancer cells taken from Henrietta Lacks, who died from her cancer on October 4, 1951. 

HeLa Human cervical cancer cell line... 

Furthermore, the induction of apoptosis in HeLa (a cell type in an immortal cell line used in scientific research it is the oldest and most commonly used human cell line and was derived from cervical cancer cells from Henrietta Lacks, a patient who eventually died of cancer) and MiaPaCa cells (human pancreatic carcinoma cells) was evident within 2 h, with the progression of cells through early and into late apoptosis. The levels of apoptosis occurring suggested that this was the dominant mode of cell death and was evident by the levels of late apoptosis/necrosis at 24 h. Mitochondria are involved in both apoptotic and necrotic death processes, influenced by the level of adenosine triphosphate and the activation of caspases [78]. 

Anonaine AT m dissipation, increase of intracellular nitric oxide, ROS, glutathione depletion, caspase activation and PARP cleavage Human cervical cancer cell Hela Alkaloid isolated from plant M c/ze/ifl alba (Magnoliaceae) [129]... 

On 1st February 1951 Flenrietta Lacks, who was suffering from cervical cancer, had - without her consent - samples of her cervix removed at the Johns Hopkins Hospital In Baltimore. She deceased on 4th October of the same year. A subset of her malignant cells was characterised and found to divide beyond the normal limit. From a single of these cells, researchers were able to establish the first Immortal human cell line, which they labelled HeLa-cells, in memory of the donor. From 1952 right up to the present time, this cell line is used worldwide for research purposes, as e.g. for the development of the polio vaccine by Jonas Salk at the University of Pittsburgh School of Medicine, which underwent clinical trials already in 1954. 

Background The assanbly of the ribosomal complex depends on the presence of proteins called enkaryotic initiation factors (elEs). In mammals the largest of these is eIF3, an -800 kDa noncovalent complex comprising of 13 stoichiometric nonidentical subunits ranging in mass from 25 to 167 kDa. This paper describes the isolation and characterization of human eIE3 obtained from HeLa cells (an inunortal cell line derived from cervical cancer cells from Henrietta Lacks in 1951). 

Preliminary biological screening pointed out that the toxicity of these systems upon light irradiation is comparable to that of cisplatin towards cervical cancer cells (HeLa). ... 

Eucalyptus ben ianui Maiden et Cambage yrtaceae) Jurkat (T leukemia cells) J774A (murine macrophage tumor) HeLa (cervical cancer) Doll-Boscardin et al. (2012... 

GA exhibits a ROS-mediated anticancer activity in human prostate cancer cells [63] and possesses antimelanogenic properties. A phenolic fraction from evening primrose Oenothera biennis) containing 55% of GA showed antitumor activity [64]. It has been proposed as a candidate for treatment of brain tumors as it suppresses cell viability, proliferation, invasion, and angiogenesis in human glioma cells. GA induces the death of HeLa cervical cancer cells via apoptosis and/or necrosis [17]. 

Pan et al. reported Tan-IIA exhibited strong growth inhibition against human cervical cancer cells (HeLa cells) in dose- and time-dependent manners with a 50% ceU growth inhibition with 2.5 pg/mL (8.49 pM). The authors executed a bioinformatics analysis of proteins to survey global protein changes induced by Tan-IIA... 

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