Cells stained

Figure 9.1. Thin section of compact bone from Schleswig (cf. Table 9.1), after application of a cell stain (methylene blue). Besides the bone cells, exogenous cellular material in the hollow spaces (shown by arrows) indicates previous microbial invasions.

The results of the lymphocyte experiments with N02 and ONOO- are given in Tables 14.4 and 14.5. The major finding is that cells that are treated with the (3-CAR in addition to vitamins E and C in vivo and exposed to N02 show the cell staining of 6.0% whereas, without the antioxidants, the cell staining was 61.4%. That is, the presence of all three of the antioxidants leads to a protection factor (PF) of 10.2. the protection by (3-CAR alone gave a PF of only 2.0, for a-tocopherol alone it was 1.8 and for ascorbic acid 1.2. 

Lymphocyte Membrane Protection by Antioxidants against N02 Cell Membrane Destruction Is Shown by Cell Staining with Eosin... 

Note For the in vitro experiments the corresponding cell staining was 5.3% and 52.9%. 

The second major finding is that cell protection was also observed against the peroxynitrite anion. Thus, in vivo, the staining increased from 5.2% with the three antioxidants to 43.3% without the antioxidants (giving a PF of 8.3). For the in vitro experiments, the corresponding cell staining was 7.3% and 59.5%, that is, a PF against ONOO- of 8.2 as shown in Table 14.5. 

In an ideal stain, the cytoplasm of cysts and trophozoites is blue-green tinged with purple. Entamoeba coli cyst cytoplasm is often more purple than that of other species. Nuclear chromatin, chromatoid bodies, erythrocytes, and bacteria stain red or purplish red. Other ingested particles such as yeasts often stain green. Parasite eggs and larvae usually stain red. Inflammatory cells and tissue cells stain in a fashion similar to that of protozoa. Color reactions may vary from the above. 

A thin, uniform layer of cells, stained by Papanicolaou and Diff-Quik methods is prepared on glass slides for microscopic examination. 

Cells Stained with Fluorescent Allelochemicals Dyes 111... 

CELLS STAINED WITH FLUORESCENT ALLELOCHEMICALS DYES... 

FIG. 5. View of the SR network in a longitudinally oriented rabbit portal vein smooth muscle cell stained with osmium ferricyanide. Note the close apposition of the SR to the plasma membrane (small arrows) and caveolae, as well as its relationship to mitochondria (M) in some instances the SR completely surrounds mitochondria. 

Fig. 13.1 Triple immunostaining of a normal duct epithelium for K14 (Ckl4, pink), K5 (Ck5/6, red), and K8/18 8 (Ckl8, green). Note that few progenitor cells are stained only for K5 and/or K14 (1), whereas most are stained both for basal (usually K5) and glandular keratins K8/18. These cells represent intermediary glandular cells. Few cells stained only for K8/18 are differentiated glandu lar cells...

Identification of these cell types is based on the morphological analysis of cells, staining for peroxidase and analysis by light and electron microscopy. Of every 100 nucleated cells in the marrow, 2 are myeloblasts, 5 are promyelocytes, 12 are myelocytes, 22 are metamyelocytes and band cells and 20 are mature neutrophils. These values are somewhat variable and may differ between individuals. Thus, about 60% of all marrow cells are of the neutrophil lineage. 

Fig. 10 (a) Fluorescence image of methanol-fixed NIH3T3 cells loaded with peptide encapsulated silver clusters for 1 h at room temperature, (b) Time profile of the time series images of cell stained with silver nitrate showing the fast silver cluster emission centered in the nucleus at short times with a maximum at 320 nm. Note that black indicates an intermediate intensity level in this color scheme [57]... 

Figure Bll.1.1 represents a 3T3 cell stained with BODIPY FL C5-ceramide (from Molecular Probes), a specific stain for the Golgi apparatus The color coding for the lifetimes is from 0 to 5 ns. The lifetime is coded in color (right upper) and this color-coded lifetime information is mapped onto the intensity surface (upper left) to give the combined lifetime/intensity plot (lower right). The final combined image shows intensity contours (in white), and a lit intensity surface is employed to accentuate the information in a three-dimensional form.

Fig. Bll.1.1. FLIM data of a 3T3 cell (stained with BODIPY FL C5-ceramide) obtained by a frequency-domain FLIM instrument (see text) .

Fig. 10.19 Lack of toxic effects of CM fullerene on breast epithelial cells. Cm does not inhibit cell proliferation. MCF 10A and (A) MDA MB 231 (B) breast cancer cell lines were cultured either in the absence or presence of methanol C60 and cell proliferation was assayed by crystal violet staining. Control, no C 1 Opg Cm, A 50 pg Cm, X 250 pg CM. (C). MDA MB 231 cells were simultaneously stained with calcein and ethidium using a live-dead assay kit. Lack of red-colored cells and the presence of cells stained in green indicate the lack of toxicity (D). MDA MB 231 cells were either untreated (open box) cultured with varying amounts 10 (gray ), 50 (patterned ) and lOOpg (filled ) of C60 for 48 h and analyzed for cell cycle progression by flow cytometry (Levi et al., 2006) (See Color Plates)...

The analysis of cell-cycle progression was one of the earliest applications of flow cytometry (for review, see Darzynkiewicz et al., 2004). In this assay, fluorescence signals from cells stained with DNA-binding fluorochromes are plotted as DNA content histograms that may be analyzed by using histogram deconvolution software to quantify cell-cycle phase distributions (Rabinovitch 1994). Fluorochromes that are useful for this purpose are the plasma membrane-impermeant DNA stains, propidium iodide (PI),... 

Figure 5 Epifluorescence microscopic images of BT 20 cells (A) Cells incubated with Rhodamine-PE labeled stearyl triphenylphosphonium liposomes (B) mitochondria in BT 20 cells stained with Mite Tracker Red. Abbreviation. PE, phosphatidylethano-lamine. Source Prom Ref. 30.

Figure 22. Human embryonic kidney cells (A), rat vascular smooth muscle cells (B, C) and human osteoblast-like MG 63 cells (D) in cultures on micropattemed surfaces. A, B PTFE irradiated with UV light produced by a Xe2 -excimer lamp for 30 min in an ammonia atmosphere through a mask with holes 100 pm in diameter and center-to-center distance 300 pm C PE irradiated with Ar ions (energy 150 keV, ion dose lO ions/cm ) through a mask with holes 100 pm in diameter and center-to-center distance 200 pm fullerenes Qo deposited through a mask with rectangular holes with an average size of 128 3 pm per 98 8 pm on glass coverslips. Day 7 after seeding. A native cells in an inverted phase-contrast microscope B, C cells stained with hematoxylin and eosin, Olympus microscope IX 50 D cells stained with fluorescence-based LIVE/DEAD viability/cytotoxicity kit (Invitrogen), Olympus microscope IX 50. Bars 300 pm (A), 200 pm (B, D), Imm (C) [10,11].

Figure 27. Human osteoblast-like MG 63 cells in cultures on porous (A) or fibrous (B) poly(L-lactide-co-glycolide) scaffolds. A A summarizing picture of horizontal optical sections. The depth of cell ingrowth into the pores (average pore diameter of 400-600 mm) is indicated by spectral colors (blue 0-60 mm, green 80-160 mm, yellow 180-220 mm, orange 240-300 mm, red 320-400 mm, violet 420-480 mm). Day 14 after seeding, cells stained with propidium iodide. B cells grown for 4 days in static culture followed by 2 days in dynamic perfusion cell culture system. Cell membrane stained with Texas Red C2-maleimide and the nuclei counterstained with Hoechst 33342. Leica TCS SP2 confocal microscope, objective 5x (A) or lOx (B) [37].

Sasaki, K. and Kurose, A. (1992) Cell staining for flow cytometry. Nippon Rinsho. 50, 2307-2311. 

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