Cell lines human

Nakamura, T., Sakaeda, T., Ohmoto, N., Tamura, T., Aoyama, N. et al., Real-time quantitative polymerase chain reaction for MDR1, MRP1, MRP2, and CYP3A-mRNA levels in Caco-2 cell lines, human duodenal enterocytes, normal colorectal tissues, and colorectal adenocarcinomas, Drug Metab. Dispos. 2002, 30, 4—6. 

Cell line human colon carcinoma cells... 

G-CSF receptors are also found on human myeloid leukaemia cells, leu-kaemic cell lines, human placenta, trophoblastic cells, vascular endothelial... 

Dibromophakellstatin (66) from the Indian Ocean sponge Phakellia mauritiana shows GIso-level growth inhibition of one-third of the NCI s 60 cell line human tumor panel at submicromolar concentrations [60]. 

On astroglial cell lines (human astrocytoma ADF cells) low (nM) concentrations of the selective A3AR agonist Cl-IB-MECA induced a marked reorganization of cell cytoskeleton accompanied by induction of expression of small GTP-binding protein of the Rho family that is involved in control of actin cytoskeleton and by changes of intracellular distribution of the antiapoptotic protein Bcl-XL (Abbracchio et al. 1997, 2001). 

Cell line (human embryonic kidney 293 cells)... 

Poon GK et al (1995) Identification of tamoxifen metabolites in human Hep G2 cell line, human liver homogenate, and patients on long-term therapy for breast cancer. Drug Metab Dispos 23 377-382... 

The method contains three stages including first establishing a cell line followed by test chemical exposure and finally evaluated for expression of cell surface markers. To establish a cell line, human leukocyte preparations are attained from a plasma distributor. The leukocyte preparations as described by Ryan et al. (2004), are diluted with an equal part of complete medium (RPMI 1640 containing 1 x L-glutamine, 1 x penicillin-streptomycin-neomycin antibiotic mixture), 30 p,2-mercaptoethanol and 10 % heat inactivated fetal bovine serum. The diluted preparation is layered onto a Ficoll-Paque gradient to... 

Human Lung Carcinoma Cell Line Human Cervical Carcinoma Human T-Cell Leukaemia Human Non Small Cell Lung Cancer Laryngeal Cancer Human Colon Cancer Human Colon Cancer... 

Green Monkey Kidney Tumor Cell Line. Human Colon Cancer Transformed Epithelial Cell Line. 

Source f, coh rWi Human lyitiphoblasioid cell line Human leuktK ylcs . voli... 

A rationale in the huge amounts of data reported in the literature is very difficult as they are far from being homogeneous. Some tests have been performed in vitro others in vivo. Furthermore different protocols and different cancer cell lines, human or not, have been used. 

The technique is reproducible, more sensitive than methods such as alkaline elution, requires fewer cells and has the important advantage that analysis can be made at the single cell level. It is therefore possible to determine heterogeneity of ISC formation and its repair in a cell population. The method is applicable to studies with cultured cell lines, human lymphocytes and solid tumor tissue. Correlations can therefore be made between both the level of ISCs and the efficiency of their repair with both inherent and acquired resistance to DNA crosslinking agents in vitro and in vivo. 

Human oral tumor cell line Human oral normal cell line ... 

Sakaguchi H, Ashikaga T, Miyazawa M, Yoshida Y, Ito Y, Yoneyame K, Hirota M, Itagaki H, Toyoda H, Suzuki H (2006) Development of an in vitro skin sensitization test using human cell lines human cell line activation test (h-CLAT). II. An international study of the h-CLAT. Toxicol In Vitro 20 774-784... 

Although the use of primary human cells will be of the highest clinical relevance, consideration may eventually be given to the use of sufficiently well-characterized and validated cell lines (human or animal) for certain aspects of the test systems. It is anticipated that most of these assays will be amenable to a microculture format, increasing efficiency and decreasing cost. 

Kirsch-Volders M, Decordier I, Elhajouji A, Plas G, Aardema MJ et al (2011) In vitro genotoxicity testing using the micronucleus assay in cell lines, human lymphocytes and 3D human skin models. Mutagenesis 26 177-184... 

Permanent and immortalized cell lines (human and non human origin) 127... 

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