HeLa cancer cells

The 5-fluorouracil (5-FU) and NONOate conjugates (Fig. 1.7) were prepared and their cytotoxicity was tested [90]. The median effect doses of the conjugates for DU145 and HeLa cancer cell lines were 2-4-fold lower than that of 5-FU. In another study by Wink et al, the cytotoxicity of cisplatin was enhanced about 60-fold after NONOate pretreatment for 30 min [91]. The enhancement of cytotoxicity of 5-FU/NONOate conjugates and cisplatin-NONOate combination has shown that there is a synergistic effect between anticancer drugs and NO. Another study by Jia et al. demonstrated that the cytotoxicity of Taxol was enhanced by S-nitrosocaptopril (Fig. 1.7) [92]. This effect is primarily mediated via the increased influx of Taxol by NO into intracellular compartments, while NO-induced cytotoxicity cannot be excluded. 

Mlerenes at 6 pM and white light to produce growth inhibition in human HeLa cancer cells. However, these same authors later reported that other carboxylic acid derivatives of C60 and C70 were completely without any photoactivity as PDT agents at 50pM (Irie et al., 1996). 

Both cyclo(Gly-Leu) and cyclo(Gly-Ile) at 10 and lOOpmolG exhibited moderate effects in inhibiting HT-29, MCF-7, and HeLa cancer cell lines, except for cyclo(Gly-Ile) that showed very little activity against HT-29 cells at a concentration of 10 pmol The greatest activity was noted for 100 pmol cyclo(Gly-Leu) against HeLa cells (28.64 1.51% inhibition, P= 0.0018). °... 

In the laboratory the immortal cell lines are those derived from cancer. The most famous is the HeLa cancer cells that many of us use as one of the cell lines tested against various anticancer agents. The name HeLa cells is derived from Henrietta Lacks, a black woman, who died from cervical cancer. The cells are so strong that they invade other cell lines, both healthy and other cancer cell lines, giving contaminated or mutated cell lines. HeLa cells have good telomerase levels. If antisense RNA is added to HeLa cells so that the RNA contains the opposite message to the ordinary RNA in the telomerase, the effect is that the telomerase is blocked and the HeLa cells are no longer immortal and die after about 25 replications. 

In 1999, Rickards et al. reported the isolation of calothrixins A (377) and B (378) from photoautrophic cultures of Calothrix cyanobacteria (345). These two, novel, pentacyclic carbazole alkaloids contain a quinolino[4,3-fc]carbazole-l,4-quinone framework. Calothrixins A and B inhibit the growth of a chloroquin-resistant strain of the malaria parasite P. falciparum and human HeLa cancer cells (345). 

Cephalotaxus wilsoniana Hayata (stems) Biflavones Kayaflavone (17) taiwanhomoflavone A (8). Cytotoxic activity Inhibitory effects against KB, Hepa-3B, and Hela cancer cell lines. Kuo et al., 2000[40]. 

Wilsoniamines A (80) and B (81) were evaluated for their antimierobial activities against malarial parasite Plasmodium falciparum and protozoan parasite Typanosoma brucei brucei and the eytotoxicity using normal HEK-293 mammalian eell line and HeLa cancerous cell line. Wilsoniamines A and B exhibited antimierobial aetivities at very higher concentration dose and did not show any cytotoxicity. 

A series of star DHBCs were evaluated for their ability to transfect hmnan cervical HeLa cancer cells with the modified plasmid pRLSV40, bearing the enhanced green fluorescent protein as the reporter gene [55]. The copolymers utilized were composed of PDMAEMA and PHEGMA blocks (where PDMAEMA is an ionizable block, while PHEGMA is a non-ionic water soluble block). The experimental data indicate a decreased toxicity for the star copolymer, compared to a reference PDMAEMA star homopolymer, for the same amounts of star polymer tested. Moreover, it has been found that the architecture of the star copolymer, i.e. star block, miktoarm star etc, plays a decisive role on the transfection efficiency. The best performance, for all star copolymers tested, was observed for a star block copolymer with... 

The volume of work on the antitumor activity and cytotoxicity of ILs and ILBSs is limited, with more publications on the effects ILs than their surface-active counterparts. Although discussion on the former class of compounds is outside the scope of the present article, we note that imidazolium-, phosphonium-, and ammonium-based ILs have been employed against 60 human tumor cell lines, including brain tumor cells, [83], human hepatocellular liver carcinoma [84], HeLa cancer cells [85], and human melanoma cell lines [86],... 

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