Cancer HeLa cervical

Finally, we wish to report the results for simulated cell smears. These experiments are being carried out to determine the best parameters for data acquisition, and to establish the variations of the cellular spectra from a homogeneous cell culture. Similar efforts have been undertaken before, where the cells were spin-deposited onto the microscope slides. However, for cultured cervical cancer (HeLa) cells, the resulting samples contained many cells that maintained their morphology in suspension quite well, and produced dried cells that were nearly spherical. Once dried, these cells could not be stained easily, and thus, their divisional activity could not be established after IR data acquisition. Furthermore, large spherical cells often gave spectral artifacts that made interpretation impossible.16... 

The transfection activity of the optimized R8-MEND was compared with Ade-noviras (see also Part I, Chapter 6), one of the strongest viral vectors, using human cervical cancer HeLa cells and a human lung epithelial carcinoma ceU hne A549 (Fig. 8.3). These ceUs display receptors for adenovirus serotype 5 such as CAR and in-... 

Another group of closely related compounds is the phenstatins. Phenstatin (6a) and hydroxyphenstatin (6b) resulted from a SAR-study on combretastatin A-4 and exhibited potent inhibition of cancer cell growth [29]. Further modifications have been performed, resulting in highly potent agents, namely 243869 (6c) with in vitro toxicity of IC5o = 0.58 nM towards HeLa cervical carcinoma cells [40,41]. 

The effect of resveratrol on the expressions of matrix metalloproteinase and tissue inhibitors of metalloproteinase in cervical cancer HeLa cells was investigated [78]. The effect of resveratrol on the invasive ability of HeLa cells was observed with a transwell cell culture chamber. Activities of MM P-2 and MMP-9 were analyzed using gelatin zymog. Activities of TIMP-1 and TIMP-2 were analyzed using reverse zymog. mRNA expressions of MMP-2, MMP-9, TIMP-1, and TIMP-2 in HeLa cells were detected by RT-PCR. Their protein expressions were detected by Western blotting. Activities of MMP-2 and MMP-9 were found to be markedly... 

Eucalyptus ben ianui Maiden et Cambage yrtaceae) Jurkat (T leukemia cells) J774A (murine macrophage tumor) HeLa (cervical cancer) Doll-Boscardin et al. (2012... 

GA exhibits a ROS-mediated anticancer activity in human prostate cancer cells [63] and possesses antimelanogenic properties. A phenolic fraction from evening primrose Oenothera biennis) containing 55% of GA showed antitumor activity [64]. It has been proposed as a candidate for treatment of brain tumors as it suppresses cell viability, proliferation, invasion, and angiogenesis in human glioma cells. GA induces the death of HeLa cervical cancer cells via apoptosis and/or necrosis [17]. 

M. Miljkovic, M. Romeo, C. Matthaus and M. Diem, Infrared microspectroscopy of individual human cervical cancer (HeLa) cells suspended in growth medium. Biopolymers, 2004, 74, 172-175. 

The first report on the anti-cancer properties of NHC-Ag complexes was made by the Youngs group and involved the use of 4,5-dichloroimidazole derived NHC-Ag. Three compounds, 5, 9 and 10, were tested against three cancer lines, OVCAR-3 (ovarian), MB157 (breast), and HeLa (cervical) (Figure 4.4). Cell viability was determined by using the MTT assay (MTT = 3-(4,5-dime-thylthiazol-2-yl)-2,5-diphenyltetrazolium bromide). IC50 values for 5, 9 and 10 on OVCAR-3 were observed to be less than 35 pM and less than 20 pM on MB 157. HeLa cells on the other hand were not affected in notable... 

The actinoporin RTX-A is strongly cytotoxic against HeLa (cervical sarcoma). THP-1 (acute monocytic leukemia), SNU-C4 (colon), MDA-MB-231 (breast), and HL-60 (lymphoblastic leukemia) human cancer cell lines, with IC50 values ranging from 1.06 to 4.66nM. 

Suresh D, Balakrishna MS, Rathinasamy K et al (2008) Large-bite bis(phosphite) ligand containing mesocyclic thioether moieties synthesis, reactivity, group 11 (Cu-I, Au-I) metal complexes and anticancer activity studies on a human cervical cancer (HeLa) cell line. Dalton Trans 2285-2292... 

In the laboratory the immortal cell lines are those derived from cancer. The most famous is the HeLa cancer cells that many of us use as one of the cell lines tested against various anticancer agents. The name HeLa cells is derived from Henrietta Lacks, a black woman, who died from cervical cancer. The cells are so strong that they invade other cell lines, both healthy and other cancer cell lines, giving contaminated or mutated cell lines. HeLa cells have good telomerase levels. If antisense RNA is added to HeLa cells so that the RNA contains the opposite message to the ordinary RNA in the telomerase, the effect is that the telomerase is blocked and the HeLa cells are no longer immortal and die after about 25 replications. 

FIGURE 8.8 A section of a single HeLa (human cervical cancer) cell stained with Phalloidin 350 (actin filaments) and MitoTracker488 (mitochondria) acquired using selective two-photon excitation, without fluorescence color filters. The image size is about 25 (xm. (From Dantus, M., Lozovoy, V. V., and Pastirk, I. Laser Focus World, 43(5) 101-104. 2007. Used with permission.)... 

Henrietta Lacks (HeLa cells immortal cell line derived from H.L. s cervical cancer cells) heteronuclear correlation (NMR) hydrophobin... 

EAG has a rather restricted distribution in healthy tissues it expresses nearly exclusively in brain, slightly in placenta and transiently in skeletal muscle (Occhiodoro et al. 1998 Pardo et al. 1999). In contrast to its restricted distribution in normal tissues, expression of EAG has been found in several human somatic cancer cell lines including HeLa cervix carcinoma, MCF-7 and SHSY-5Y neuroblastoma derived from breast tumor, melanoma IGR1, rhabdomyosarcoma, etc. (Meyer 1998, 1999 Crociani 2003). Most notably, EAG mRNA expression was found in 100% of the human cervical cancer sam-... 

Butter yellow is active in inducing unscheduled DNA synthesis in Hela human cervical cancer cells. It was mutagenic in Salmonella typhimurium TAIOO and TA98. 

In Vitro. Mouse connective tissue (L cells), human cervical carcinoma (HeLa), and human amnion (WISH) cancer cells were used to determine the toxic and nontoxic levels of several of the... 

Ehrlich tumours are poorly differentiated malignant tumours (originally a breast carcinoma in a mouse), which grow in solid and ascetic forms. HeLa cells are a cell line developed from cervical cancer of a woman ( enriette Lacks), who died from that cancer in 1951. 

In vitro cytotoxic analysis of the main saffron compounds (crocins, crocetin, picrocrocin and safranal) using HeLa (human cervical epitheliod cancer) cells was carried out by Escribano et al [64]. It was found that in terms of LD50 values (dose inducing 50% cell growth inhibition on these cells) safranal has 0.8mM, crocin and picrocrocin 3mM respectively. Furthermore, Escribano et al [65] have also isolated from corms of Crocus sativus a glycoconjugate with cytotoxic activity as well. Experiments with carotenoids from Crocus sativus in HL60 cells were conducted by Tarantilis et al [66] where inhibition and induction of differentiation of these cells was studied. 

A HeLa cell (also Hela or hela cell) is an immortal cell line used in medical research. The cell Une was derived from cervical cancer cells taken from Henrietta Lacks, who died from her cancer on October 4, 1951. 

Zacharewski et al. (1998) found that di- -butyl phthalate induced reporter gene (luciferase) activity in MCF-7 human breast cancer cells, but not HeLa human cervical carcinoma cells, transfected with human estrogen receptor, although approximately 3,000-fold less potent than 17p-estradiol. Additionally, Zacharewski et al. (1998) found that di- -butyl phthalate was capable of supporting a very modest amount... 

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