Adenovirus serotypes

Devaux, C., Adrian, M., Berthet-Colominas, C., Cusack, S., andjacrot, B. (1990). Structure of adenovirus fibre. I. Analysis of crystals of fibre from adenovirus serotype 2 and 5 by electron microscopy and X-ray crystallography./ Mol. Biol. 215, 567-588. 

Louis, N., Fender, P., Barge, A., Kitts, P., and Chroboczek, J. (1994). Cell-binding domain of adenovirus serotype 2 fiber./ Virol 68, 4104—4106. 

Leopold, P.L., Kreitzer, G., Miyazawa, N., Rempel, S., Pfister, K.K., Rodriguez-Boulan, E. et al. (2000b) Dynein- and microtubule-mediated translocation of adenovirus serotype 5 occurs after endosomal lysis. Hum. Gene Ther., 11, 151-165. 

To localize the RGD residues on the Ad penton base, a cryo-EM reconstruction was performed of adenovirus type 2 (Ad2) complexed with an RGD-specific Eab fragment from an mAb directed against the penton base (Stewart et al, 1997). This structural analysis revealed that the RGD regions are at the top of protrusions on the pentameric penton base protein. In addition, it was deduced from the diffuse nature of the Fab density that the RGD residues were in a structurally variable surface loop. Comparison of the knovra sequences of the penton base protein from various adenovirus serotypes suggested that type 12 adenovirus (Adl2) would have the least structurally variable RGD loop, as Ad 12 has 45 fewer residues in the variable region flanking the conserved RGD residues than are found in Ad2 (Chiu et al, 1999). 

The PER.C6 human ceU line was generated by the immortahzation of primary retina cells with El sequences of human adenovirus serotype 5. The cell hne was initially developed for the safe production of phar-... 

The transfection activity of the optimized R8-MEND was compared with Ade-noviras (see also Part I, Chapter 6), one of the strongest viral vectors, using human cervical cancer HeLa cells and a human lung epithelial carcinoma ceU hne A549 (Fig. 8.3). These ceUs display receptors for adenovirus serotype 5 such as CAR and in-... 

Vector Construction. Construction of the recombinant replication incompetent (El/2 deleted) adenovirus serotype 5 (Ad5) expressing the human FGF-4 cDNA required three components the FGF-4 transgene, a plasmid shuttle vector to carry the transgene as well as 5 Ad5 sequences, and a second plasmid carrying the bulk of the Ad5 genome. 

Seshidhar Reddy P, Ganesh S, Limbach M P, et al. (2003). Development of adenovirus serotype 35 as a gene transfer vector. Virol. 311 384-393. 

Lemckert A A, Sumida S M, Holterman L, et al. (2005). Immunogenicity of heterologous prime-boost regimens involving recombinant adenovirus serotype 11 (Adll) and Ad35 vaccine vectors in the presence of anti-ad5 immunity. J. Virol. 79 9694-9701. 

Horton TM, Tollefson AE, Wold WS, Gooding LR (1990) A protein serologically and functionally related to the group C E3 14,700-kilodalton protein is found in multiple adenovirus serotypes. J Virol 64 1250-1255... 

Roelvink PW, Lizonova A, Lee JGM, Li Y, Bergelson JM, Finberg RW, Brough DE, Kovesdi I, Wickham TJ (1998) The coxsackievirus-adenovirus receptor protein can function as a Cellular attachment protein for adenovirus serotypes from subgenera A, C, D, E, and F. J Virol 72 7909-7915... 

Indeed, the whole question of the variable responses of mammalian cells to different human adenovirus serotypes is poorly understood, despite the fascinating range of interactions displayed. The variable abilities of cells derived from different species, or different tissues, to support adenovirus replication is generally interpreted in terms of the presence, or lack of, cellular factors that the virus needs to complete various steps in the replication cycle. More precisely, putative factors that permit human adenovirus replication in human cells may be present in, say, murine cells, but sufficiently divergent that they fail to interact optimally with the relevant viral components. Thus, an understanding in molecular terms of the steps in the viral replication cycle that are blocked in non- or semipermissive cells should provide important information about the host cell components utilized by the virus and, thus, the molecular interactions among viral and cellular products. 

In another mouse model of cancer, IMO has been combined with a genetic vaccine targeting murine TERT (mTERT) based on DNA electroporation (DNA-EP) and Adenovirus serotype 6 (Ad6) [101]. In this model, IMO induces dose-dependent cytokine secretion and activation of NK cells. Most importantly, vaccination of mice with IMO in combination with mTERT vaccine confers therapeutic benefits in tumor-bearing mice. Importantly, the therapeutic effect of the combination of mTERT vaccine and IMO correlates with increased NK, DC, and T-cell infiltration into the tumor microenvironment, suggesting the benefit of the combination of vaccine with IMO [101]. 

Gall JGD, Cryslal RG, Falck-Pederson E. Construction and characterization of hexon-chimetic adenoviruses specification of adenovirus serotype. J Virol 1998 72 10260-10264. 

Ostapchuk P, Hearing P. Pseudopackaging of adenovirus type 5 genomes into capsids containing the hexon proteins of adenovirus serotypes B, D, or E. J Virol 2001 75 45-51. 

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