Microscope slides

The first work published in this area was that of Bigelow mentioned above [116], In 1957, monolayers of long-chain fatty acids were fonned on thin films of silver, copper, iron and cadmium deposited on glass microscope slides [43],... 

If it is desired to observe the crystalline form of the osazone, draw up in a glass tube a few drops of the cold filtrate containing the fine crystals, and transfer to a microscope slide. Cover the drops with a slip and examine under the microscope unless the filtrate has been cooled very slowly and thus given moderately-sized crystals, the high power of the microscope will probably be required. Note the fine yellow needles aggregated in the form of sheaves. Compare with Fig. 63(A). 

Microscope appearance. Place a small amount of dry starch on a microscope slide, add a drop of water, cover with a slip and examine under the microscope. Characteristic oval grains are seen which have concentric rings round a hilum which is towards one end of the grain. Run a drop of very dilute iodine solution under the slip from a fine dropping-tube the grains become blue. 

Artifacts introduced through sample preparation are common materials these may be bits of facial tissue, wax, epithelial cells, hair, or dried stain, all inadvertently introduced by the microscopist. Detergent residues on so-called precleaned microscope slides and broken glass are common artifacts, as are knife marks and chatter marks from sectioning with a faulty blade, or scratch marks from grinding and polishing. 

Microscopic slides of a certain culture of microorganisms contain on the average 20 microorganisms per square centimeter. After treatment by a chemical, one square centimeter is found to contain only 10 such microorganisms. If tlie treatment had had no effect, what would be the probability of finding 10 or fewer niicroorganisnis in a given square centimeter ... 

Filtration Technique. A measured quantity of sample water is filtered through the membrane filter. The filter is dried, cut and sections placed on microscope slides. The filter sections are rendered transparent by a drop of immersion oil. The slides are examined to identify the microorganisms listed below ... 

The technique used to study dewetting dynamics on materials consists of making a flat, smooth elastomer surface. A hquid puddle is deposited within a 50-mm-diameter ring of 0.1-mm-thick plasticized adhesive paper adhering to the substrate. The adhesive paper acts as a spacer. A microscope slide is drawn over the liquid to obtain a liquid film of ca. 0.1-mm thickness. At this thickness, the liquid film is unstable, being much less than the equilibrium value, of ca. 1.5 mm calculated from Eq. (29). Nucleation of dry patches... 

Particles become positively charged by a corona discharge and travel out of the charging chamber and collect on a substrate such as a microscope slide. Thus, the method is useful for particles which are to be examined by optical or electron microscopy. 

In this case, three particles are shown, a 40 u, 20 p and a 10 p particle. The most important step is sample preparation on the microscope slide, since only a pinch of materied is used, one must be sure that the sample is uniform and representative of the material. Also, since most materials tend to agglomerate due to accumulated surface charge in a dry state, one adds a few drops of alcohol and works it with a spatula, spreading it out into a thin layer which dries. Too much working breaks down the original peirticles. 

To study reaction kinetics, cement batches of total mass 300 g were prepared using ingredients measured to the nearest 0-1 g. Mixing was carried out for 10 minutes using a kitchen blender, after which specimens were cast in slabs 10 x 10 x 1-2-1-5 cm in polyethylene moulds. When the setting reaction had proceeded to a sufficient extent and viscosity had risen to give a reasonably stiff paste, a small portion was removed, placed on a glass microscope slide and immediately examined by X-ray diffraction. The remainder of the sample was allowed to set. 

Sputum smears Lower respiratory tract smear on a microscope slide to determine the presence of organisms. 

Polymer Films. The polymer films were prepared by casting 5% toluene solutions onto glass microscope slides. After air drying in a fume hood, the films were vacuum dried. Film thicknesses were typically 0.05 mm. 

Surface Modification. A polydiene film (supported on a microscope slide) was immersed in a stirred, room temperature, RTD-acetonitrile solution of known concentration contained in a large glass-stoppered test tube. After a specific reaction time, the film was removed from the solution, washed with acetonitrile, water, and acetonitrile again, and dried under vacuum (Step 1). Films subsequently treated with base were immersed in aqueous solutions for 5-15 min. They were then washed with water and CH3CN, and vacuum dried (Step 2). Some films were aged in air at room temperature. 

As an example (and this is a hypothetical example only), a particle is shown in Fig. 11 such as might appear on a microscope slide. This particle is gridded out in the form shown in the upper lefthand corner of Fig. 11. The number of squares in which parts of the trace of the particle is located is counted. This number is N, and the length of the grid size is g. The grid size is arbitrarily set equal to one in this example. The grid... 

Figure 7.1 Schematic illustration of eight peptide controls, in a 2 x 4 array, on a glass microscope slide that also contains the patient s tissue sample.

Figure 7.4 is a photograph of a portion of the peptide controls slide printer. A stack of microscope slides, ready for printing, is at the far left. The slides are automatically ejected from the stack, one at a time. The slides are moved on a conveyer to the right, positioning them under the print head. The print head has eight nozzles, out of which microliter-sized droplets are ejected onto an underlying slide. The slide conveyer then places a new slide under the nozzles, and the process repeats. 

Sompuram SR, McMahon D, Vani K, et al. A novel microscope slide adhesive for poorly adherent tissue sections. I. Histotechnol. 2003 26 117-123. 

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