Rauscher virus

Antiviral activities Inhibition of herpes simplex virus Inhibition of Rauscher virus NIH/3T3 cells Inhibition of herpes simplex virus Inhibition of herpes simplex virus Inhibition of herpes simplex virus Inhibition to Rauscher virus NIH/3T3 cells Inhibition of herpes simplex virus Inhibition to Rauscher virus NIH/3T3 cells Inhibition to Rauscher virus NIH/3T3 cells Inhibition of herpes simplex virus Inhibition of murine sarcoma virus Inhibition to Rauscher virus NIH/3T3 cells Inhibition of herpes simplex virus Inhibition to Rauscher virus NIH/3T3 cells Inhibition to Rauscher virus NIH/3T3 cells Inhibition of herpes simplex virus Inhibition to Rauscher virus NIH/3T3 cells Inhibition of herpes simplex virus Inhibition of potato x virus... 

Basic science research at The Section/DM. The NIH/NCI grant-supported retroviral mouse leukemia work at the Section of Clinical Tumor Virology Immunology included the early study of graft-versus-leukemia reactions in Rauscher virus-infected mice [261a, b]. 

Lycorine (8) inhibits ascorbic acid synthesis (Robinson, 1979) as well as plant cell division and cell elongation (Martin, 1987). This alkaloid also inhibits DNA polymerase, protein synthesis, Rauscher virus NIH/3T3, and herpes simplex virus (Wink, 1993). 

Narciclasine (22) halts protein synthesis by blockage of peptide-bond formation on the 60-S ribosomal subunit. The effect is specific for eucaryotic cells. Narciclasine inhibits Rauscher virus NIH/3T3 (Wink, 1993). Lycorine (8) blocks mitosis in the broad bean (Viciafaba). The mechanism appears to be related to inhibition of protein synthesis (Suffness and Cordell, 1985). Pretazettine (15) has been used in combination with DNA-binding and alkylating agents in the treatment of the Rauscher leukemia virus (Cordell, 1981 Martin, 1987). This alkaloid inhibits purified RNA-dependent DNA polymerase (reverse transcriptase) from avian myelo-... 

The LD50 i.v. of tazettine (25) in mouse is 100 mg/kg (Wink, 1993). Lycorine (8), haemanthamine (14) and pseu-dolycorine (26) are toxic to Rauscher virus NIH/3T3 cells and inhibit protein biosynthesis (Wink, 1993). 

Rauscher virus RN A From whole virions as for None Gel electrophoresis Bloemendal and... 

Administration of exogenous interferon is reported to exert a protective effect in tissue culture against adeno-2 virus and in mice has prolonged life or prevented death caused by Rauscher virus , vesicular stomatitis virus and encephalonyocarditis virus. There are reports that some interferons could cross species lines and be effective unlike earlier evidence of greater specificity. 

PMEA and its congeners are more effective in vivo than could be predicted from their in vitro potency. While less potent as an antiretrovirus agent than AZT in vitro, PMEA proved clearly superior to AZT when the two drugs were compared for their effectiveness in vivo, in mice infected with murine Moloney sarcoma virus [51,52]. PMEA was also shown to be effective against various other retrovirus infections, including Friend leukemia virus (FLV), Rauscher leukemia virus (RLV), and LP-BM5 (murine AIDS) virus infection in mice, feline leukemia virus (FeLV) or feline immunodeficiency virus (FIV) infection in cats, and SIV infection in macaque (rhesus) monkeys (for review, see Ref. 53). In the latter model [54], again PMEA proved far superior to AZT in suppressing several parameters of the disease. 

Dunkel VC, Pienta RJ, Sivak A, et al. 1981. Comparative neoplastic transformation responses of Balb/3T-3 cells, Syrian hamster embryo cells, and Rauscher murine leukemia virus-infected Fischer 344 rat embryo cells to chemical carcinogens. J Nat Cancer Inst 67 1303-1315. 

Schwendener RA, et al. New lipophilic acyl/alkyl dinucleoside phosphates as derivatives of 3 -azido-3 -deoxythymidine Inhibition of HIV-1 replication in vitro and antiviral activity against Rauscher leukemia virus infected mice with delayed treatment regimens. Antivir Res 1994 24 79. 

AntivertigO effect. Powdered rhizome, administered orally to human adults at a dose of 1 g/person, was active vs seasickness ° k Rhizome, administered orally to adults of both sexes at a dose of 1 g/person, was inactive. The treatment was administered 2 hours before the subjects were rotated in a chair making head movement until a symptom short of vomiting was reached ° Antiviral activity. Aqueous low-speed supernatant of the rhizome, at a concentration of 1% and the rhizome juice, produced strong activity on virus-top necrosis h Decoction of the rhizome together with c at a concentration of 250 pg/mL, was active on HlV-1 and Rauscher murine leukemia viruses. Reverse transcriptase activity was inhibited . Water extract of the rhizome, in cell culture at a concentration of 10%, was inactive on herpes virus type 2, A2-... 

Reverse transcriptase inhibition. Decoction of the rhizome, in cell culture, was active on Rauscher murine leukemia virus. The study was conducted with a prescription consisted of Bupleurum falcatum (Rt), Scutellaria baicalensis (Rt), Pinellia ternata (Tu), Zizyphus jujuba (Fr), Panax ginseng (Rt), and Glycyrrhiza glabra (Rh) h Serotonin antagonist activity. Acetone extract of the rhizome, at a concentration of 25 pg/mL, was active on the guinea pig ileum vs serotonin-induced contract-ions ° . 

Total syntheses of chrysin (93) and luteolin (94), which are non-toxic potent inhibitors of reverse transcriptases from Rauscher murine leukemia (RLV) and the human immunodeficiency virus (HIV), were accomplished by Antus and coworkers via cyclodehydrogenation of the appropriately substituted 2 -hydroxy-chalcone (179) in the presence of PIDA/KOH in MeOH [136] (Scheme 45). 

Pretazettine (395) has been the subject of numerous biological studies, and it has been shown to exhibit a number of interesting activities (96,97,101,178-187). For example, 395 was found to inhibit HeLa cell growth as well as protein synthesis in eukaryotic cells by interfering with the peptide bond formation step (97,101). Furthermore, pretazettine inhibited the purified RNA-dependent DNA polymerase (reverse transcriptase) from avian myeloblastosis virus, a typical C-type virus (178), in an unusual fashion since it physically combined with the polymerase enzyme itself rather than interacted with the nucleic acid template. Pretazettine also exhibited antiviral activity against the Rauscher leukemia virus in mouse embryo cell cultures by suppressing viral replication (179). 

Raman spectroscopy, 67 Rauscher murine leukemia virus, electron spin resonance (ESR) and, 51... 

Whole viruses and virus proteins AKR Moloney Rauscher Scripps leukemia Vaccinia... 

Polymer (VI) has been shown to have antitumor activity against adenocarcinoma 755, Dunning ascites leukemia. Friend leukemia virus, and Lewis lung carcinoma. In the latter case, polymer (VI) showed activity about equal to that of cyclophosphamide (an alkylating agent) and was more effective than 6-mercaptopurine (an antimetabolite) ( ). The DIVEMA polymer (V) Is also active against some cancer causing viruses such as Friend leukemia, Moloney sarcoma and Rauscher leukemia ( ). 

Castanospermine has been screened for efficacy against simian immunodeficiency virus (265), and has been shown to prevent syncytium formation in feline astrocyte cultures infected with the feline immimodeficiency virus by modifying the viral cell envelope (266). It suppressed syncytium formation and hemolytic activity in baby hamster kidney cells infected with Newcastle disease virus however, synthesis and cell surface expression of the hemagglutinin-neuraminidase glycoprotein in the viral envelope were not affected, which strengthens the hypothesis that poor transport of the parent alkaloid across membrane barriers may limit its therapeutic use (267). Both 239 and its 6-0-butanoyl ester had comparable relative toxicities and antiviral effects on Rauscher murine leukemia virus (268), but the ester was more potent than the parent alkaloid in inhibiting replication of Moloney murine leukemia virus (258). The ester was also active against herpes simplex viruses types 1 and 2 (269,270). In the latter case, conclusive evidence was provided for intracellular hydrolysis to 239. 

Among the naphthalenoid ansamycin antibiotics, the streptovaricin complex was reported to inhibit focus formation by MSV (MLV murine leukaemia virions) and to inhibit the splenomegaly-induced Rauscher leukaemia by virus selectively [226,227]. On the other hand, awamycin (26) [34,35] was shown to possess activity against HeLa S3 cells and antitumor activity against experimental murine tumors. Antitumor activity tests on streptovaricin C (44) were conducted [226,227]. 

---