Baby hamster kidney-21 cells

In the procedure for the surface test (313), the vims is grown in a monolayer of baby hamster kidney cells and incubated in Eagles medium supplemented with tryptose phosphate broth and calf semm. After separation of the vims from the cells by sonification and centrifugation, amounts of the suspension containing 3 x 10 plaque-forrning units are dried on coversHps. The inoculated coversHps are placed in 5 ml of the disinfectant for 1, 5, or 10 min, then rinsed, sonicated, and assayed. 

Another application shows the preparative purification and polishing of a therapeutic fusion protein with a humanized recombinant IgG protein. The fusion protein was expressed by the fermentation of baby hamster kidney cells. The filtered culture supernatant (155 liters) contained 2.2 g of IgG and 75.5 g of total protein. After the immunoglobulins were isolated by expanded bed adsorption and rebuffering, the IgG fraction was bound to Fractogel EMD SOj (M). This column achieved baseline separation of complete antibodies (fusion protein) from small amounts of antibodies lacking the fusion part. The resulting highly purified IgG fraction (110 ml) was diluted to 150 ml and... 

Kratje, R. B., Reimann, A., Hammer, J., and Wagner, R., Cultivation of Recombinant Baby Hamster Kidney Cells in a Fluidized Bed Bioreactor System with Porous Borosilicate Glass, Biotechnol. Prog., 10 410 (1994)... 

In addition to fluorescence methods, another study [27] developed a method to permit electron microscopic localization of Ras anchor domains on cytoplasmic membrane surfaces by immunogold labeling. The particle neighbor distances can be analyzed to obtain information about possible domain structure. Expressing H-Ras and K-Ras in baby hamster kidney cells, a nonrandom particle distribution was obtained from which the estimated mean raft size was 7.5-22 nm and about 35% of the membrane area consists of rafts. The same technique applied to cells that had been incubated with [3-cydodextrin to reduce cholesterol produced completely random distributions of H-Ras. This cholesterol dependence suggests some type of coupling of rafts across the inner and outer membrane leaflets. 

Li+ has significant inhibitory effects upon DNA viruses, in particular HSV which has been studied in depth. It was originally shown that Li+ inhibits viral replication in a dose-dependent, reversible manner in HSV-infected baby hamster kidney cells [240], and this has been found to be due to a Li+-induced decrease in the synthesis of viral DNA [241]. It is now well established that Li+ inhibits DNA synthesis in HSV types 1 and 2 and in several other DNA viruses, including measles, vaccinia, adenovirus, poxvirus, pseudorabies virus, Epstein-Barr virus, and the bovine, equine, and canine HV s [241]. Interestingly, Li+ has no effect on the replication of RNA viruses, such as influenza or encephalomyo-carditis virus. 

M. C. Gehrmann, M. Opper, H. H. Sedlacek, K. Bosslet, J. Czech, Biochemical Properties of Recombinant Human /3-Glucuronidase Synthetized in Baby Hamster Kidney Cells , Biochem. J. 1994, 301, 821 - 828. 

Bacterial hosts are inappropriate choices for expression of proteins such as the blue copper proteins stellacyanin, laccase, and ceruloplasmin which are extensively glycosylated. In these cases, it may be necessary to employ tissue cultures of appropriate origin to obtain the native protein. In this regard, the amino-terminal half of human serum transferrin, which lacks carbohydrate, has been expressed in high yield in baby hamster kidney cells by Funk et al. [13], while the glycosylated carboxyl-terminus has proved to be more problematic [103]. 

The reader should be aware that other types of cells expressing CD 154 have been used to stimulate CLL cells. These include mouse fibroblast L-cells (NTL) (12, 20, 21, 23, 28) and baby hamster kidney cells (27). The CD 154 NIH3T3 cells used in this study were kindly provided by Dr. Eldering (Department of Pathology, Academic Medical Center, Amsterdam, The Netherlands). 

Table 3.9. Some biopharmaceuticals currently on the market that are produced by genetic engineering in either E. coli or animal cells. CHO = Chinese hamster ovary cells BHK = baby hamster kidney cells...

Danford, N. (1991) The genetie toxicology of or//zo-toluidine. Mutat. Res., 258, 207-236 Daniel, M.R. Dehnel, J.M. (1981) Cell transformation test with baby hamster kidney cells. In de Senes, F.J. Ashby, J., eds, Progress in Mutation Research, Volume 1, Evaluation of Short-Term Tests for Carcinogens. Report of the International Collaborative Program, Amsterdam, Elsevier Seienee, pp. 626-637... 

Baby hamster kidney cells were genetically modified to secrete high levels of human NGF before undergoing polymer encapsulation prior to implantation. Although the therapy showed potential for the treatment of neurological disorders, the emergence of stem cells offered far broader therapeutic scope. 

The genetic manipulation of animal cells allows the production of therapeutic proteins in animal cell culture systems. Mammalian cells such as Chinese hamster ovarian cells and baby hamster kidney cells are commonly used. These mammalian hosts produce recombinant proteins that have almost identical properties to those made by human cells. However, the use of mammalian cells does have disadvantages. As noted earlier, they are expensive to use. This is influenced by their more complex nutritional requirements, their slower growth, and their increased susceptibility to physical damage (Walsh, 2003). 

The BHK-21 (baby hamster kidney) cell line consists of adherent fibroblasts, that can also be adapted to suspension culture, and was isolated from five 1-day-old hamsters (McPherson and Stoker, 1962). These cells are commonly used for virus propagation (polio, rabies, and foot-and-mouth disease) for production of veterinary vaccines. 

ADA BEVS BHK CHO ELISA mAb MDCK MMR SCID tPA VLP adenosine deaminase deficiency baculovirus expression vector system baby hamster kidney cell line Chinese hamster ovary cell line enzyme linked immuno sorbent assay monoclonal antibodies Madin-Darby canine kidney epithelial cells measles, mumps, rubella severe combined immunodeficiency plasminogen activator virus-like particle... 

ATP EDTA DMSO BHK CHO HEPES mAbs B Brnax MTT PEG RNA t td WHO X adenosine triphosphate ethylenediaminetetraacetic acid Dim ethylsulf oxide baby hamster kidney cell line Chinese hamster ovary cell line N-(2-hydroxyethyl)piperazine-N -(2-ethanesulphonic acid) monoclonal antibodies specific cell growth rate maximum specific cell growth rate (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide) polyethylene glycol ribonucleic acid cell culture time cell doubling time World Health Organization cell concentration... 

Acetyl-CoA ADP Ala AMP ATP BHK CC9C10 CHO CHSE CMP-NANA CoQ CoQH2 DNA acetyl-coenzyme A adenosine diphosphate alanine adenosine monophosphate adenosine triphosphate baby hamster kidney cell line hybridoma cell line Chinese hamster ovary cell line Chinook salmon embryo cell line cytosine monophosphate-N-acetylneuraminic acid coenzyme Q dihydroubiquinone deoxyribonucleic acid... 

ATCC BHK cells BME BPL BRL BSA BSS BUdr CHO cells CMF CMP, CDP, CTP American Type Culture Collection baby hamster kidney cells basal medium (Eagle) /J-propiolactone Buffalo rat liver bovine serum albumin balanced or buffered salt solution bromodeoxyuridine Chinese hamster ovary cells calcium- and magnesium-free BSS cytidine monophosphate, diphosphate, triphosphate... 

PS - cell-free wheat (6), rabbit reticulocyte (7) [baby hamster kidney cell PS inhibition (0.04-0.4)]... 

ZnT-2, a component of vesicular acid intracellular compartments, is predominantly expressed in intestine, kidney, and testis and is scarcely detected in brain in mice (Palmiter et al., 1996). Overexpression of ZnT-2 in baby hamster kidney cells conferred resistance to elevated Zn with sequestration into acidic compartments at higher concentrations (Palmiter et al 1996). In contrast, coexpression of ZnT-1 suppressed ZnT-2 mediated transport into acidic vesicles suggesting ZnT-2 has a relatively low affinity for Zn and functions only under excessive elevations of Zn as a second line of defense when other ZnTs fail to function properly (Palmiter et al., 1996). ZnT-3 sequesters Zn in vesicles and has expression limited to brain and testis (Palmiter et al., 1996). In mouse brain, ZnT-3 is associated with hippocampal dentate granule cells, pyramidal, and intraneurons as evidenced by levels of mRNA (Palmiter et al., 1996). 

Polatnick J Bacharach HL (1964) Production and purification of milligram amounts of foot-and-mouth disease virus from baby hamster kidney cell culture. Applied Microbiology 12 368-373. 

Holothurinosides A (75), C (76) and D (77) from Holothuria forskali and the desulfated derivative 78 at a concentration 20 )Xg/ml caused an inhibition of cytopathic effect induced by vesicular stomatitis virus (VSV) in cell culture (20% inhibition of VSV in baby hamster kidney cell line) [68]. 

Baby hamster kidney cells Cell transformation - No data Styles 1981... 

Dana MT, Lee RN, Hales JM. 1985. Hazardous air pollutants Wet removal rates and mechanisms. Richland WA U.S. Environmental Protection Agency. EPA 600/3-84-113. NTIS PB85 138-626. Daniel MR, Dehnel JM. 1981. Cell transformation test with baby hamster kidney cells. Mutat Res... 

Daniel MR, Dehnel JM. 1981. Cell transformation test with baby hamster kidney cells. Mutat Res 1 626-637. 

Castanospermine has been screened for efficacy against simian immunodeficiency virus (265), and has been shown to prevent syncytium formation in feline astrocyte cultures infected with the feline immimodeficiency virus by modifying the viral cell envelope (266). It suppressed syncytium formation and hemolytic activity in baby hamster kidney cells infected with Newcastle disease virus however, synthesis and cell surface expression of the hemagglutinin-neuraminidase glycoprotein in the viral envelope were not affected, which strengthens the hypothesis that poor transport of the parent alkaloid across membrane barriers may limit its therapeutic use (267). Both 239 and its 6-0-butanoyl ester had comparable relative toxicities and antiviral effects on Rauscher murine leukemia virus (268), but the ester was more potent than the parent alkaloid in inhibiting replication of Moloney murine leukemia virus (258). The ester was also active against herpes simplex viruses types 1 and 2 (269,270). In the latter case, conclusive evidence was provided for intracellular hydrolysis to 239. 

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