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Nucleic acid template

Streptavidin-single-stranded DNA covalent conjugates were described as the building blocks for assembling nanostructured scaffolds [31], The amount and type of biotinylated ligands were used to modulate the affinity of duplex formation between solid-phase-bound nucleic acid templates and DNA-streptavidin conjugates. This system has been proposed for the design of fine-tuned sequence detection systems. [Pg.434]

Leal, N. A. Sukeda, M. Benner, S. A. Dynamic assembly of primers on nucleic acid templates. Nucleic Acids Res. 2006, 34,4702 710. [Pg.117]

Some may say at this point that proteins derive in any case from nucleic-acid templates - perhaps through a primitive genetic code. However, this is really no argument - it merely shifts the problem of the etiology of peptide chains to etiology of oligonucleotide chains, all arithmetic problems remaining more or less the same. [Pg.70]

Calderone, C. T. and Liu, D. R. (2004). Nucleic acid-templated synthesis as a model system for ancient translation. Curr. Opin. Chem. Biol, 8, 645-53. [Pg.275]

A close-up view showing the relative locations of the commonly identified drug-resistance mutations for NRTIs (in dark-gray) and for NNRTIs (in light-gray) with respect to the bound DNA. Most of the NRTI-resistance mutations are not located at the putative dNTP-binding site, but are at positions to have potential interactions with the nucleic acid template-primer. Conversely, all the NNRTI-re si stance mutations are clustered around the NNIBP and have direct contacts with NNRTIs or have direct effect on... [Pg.53]

Nucleic Acid Templates, The Kinetics of Biopolymerization on (Gibbs) 15 185... [Pg.386]

Pretazettine (395) has been the subject of numerous biological studies, and it has been shown to exhibit a number of interesting activities (96,97,101,178-187). For example, 395 was found to inhibit HeLa cell growth as well as protein synthesis in eukaryotic cells by interfering with the peptide bond formation step (97,101). Furthermore, pretazettine inhibited the purified RNA-dependent DNA polymerase (reverse transcriptase) from avian myeloblastosis virus, a typical C-type virus (178), in an unusual fashion since it physically combined with the polymerase enzyme itself rather than interacted with the nucleic acid template. Pretazettine also exhibited antiviral activity against the Rauscher leukemia virus in mouse embryo cell cultures by suppressing viral replication (179). [Pg.327]

A short introduction to peptide synthesis will be given before the synthesis of glycopeptides is discussed. Proteins are assembled by a nucleic acid templated reaction from a menu comprising 19 L-oc-amino acids of general structure 1 and the amino acid L-proline (2) (Figure 5.1). Nature synthesises proteins by a stepwise assembly of amino acid building blocks. Since these compounds are readily available in... [Pg.156]

Gibbs EJ, Tinoco I, Maestre M et al (1988) Self-assembly of porphyrins on nucleic acid templates. Biochem Biophys Res Commun 157 350-358... [Pg.187]

Berberine chloride exhibited reverse transcriptase inhibitory properties against both H1V-1RT (p66/p51)(Human immunodeficiency virus type 1 reverse transcriptaseXIC50 60.9 pg/ml [163.8 (J.M]) and HIV-2RT (p68/p55)(Human immunodeficiency virus type 2 reverse transcriptase)(IC o 57.8 pg/ml [155.5 pM]). The alkaloid is thought to inhibit various RNA and DNA polymerizing enzymes via interaction with nucleic acid template-primers [212]. [Pg.127]

Weiner. A. VI. 2004. tKNA maturation RN.A polymerization wiihmit a nucleic acid template. Curr. HioL 14 RS83 RS85,... [Pg.854]

Among the various methods that can be applied to entrap macromolecules inside liposomes, those methods have to be selected that increase the entrapment efficiency substantially and lead to an entrapment efhciency that is higher than the entrapment yield that results from vesicles prepared by dispersing a lipid hhn with subsequent extrusion (VET method, see Reference 7). Entrapment of macromolecules by the VET method is quite inefficient the likelihood that one liposome could contain enzyme as well as nucleic acid template molecule is very low. [Pg.612]

Autocatalytic systems have been proposed to be instigator of the first life. In this example the autocatalytic replication of nucleic acids generates the nucleic acid template for its own reproduction (55) in this case a hexadeoxynucleotide replicates. In the optimal case the autocatalytic system replicates exponentially resulting in a very rapid build up of the nucleotide. Exponential growth has not been attained in autocatalytic systems proposed to initiate the first life 66). [Pg.305]

The term isozyme was originally defined as meaning enzymes with a different primary structure, catalyzing the same reaction, encoded by different genes. However, the term is now used more broadly. Some isozymes are now known to arise from alternate splicing of pre-mRNA to form different mRNAs (mRNA is the final nucleic acid template used for protein synthesis.)... [Pg.83]

Weizmann, Y., Cheglakov, Z., Pavlov, V., Willner, I. (2006a). Autonomous fueled mechanical replication of nucleic acid templates for the amplified optical detection of DNA. Angew Chem Int Ed Engl 45, 2238-2242. [Pg.100]

The widespread use of PCR amplification in microarray manufacture places much importance on the availability of informatics tools for automated primer design. A primer is a synthetic oligonucleotide that hybridizes to a complementary nucleic acid template, and expedites (primes) enzymatic synthesis by providing a starting point for enzyme. PCR primers bind to proximal and distal sites on cDNA templates, allowing target sequences... [Pg.578]

Nucleic acid templates should be added to reactions last (in the designated template area). The recommended order of steps in standard reaction is Assembly-Template Addition-Incubation-Analysis. All tubes should remain sealed once all reagents have been added, and be reopened for analysis only (in an area separate from all reagents and reaction assembly). [Pg.259]

Scheme 4 a The rearrangement-elimination process between tetrazine and TCO-2-enol reaction, b The tetrazine-mediated transfer reaction. Reaction half-life was measured in a nucleic acid-templated reaction... [Pg.116]

Template-directed synthesis allows the formation of certain products that are difficult to synthesise chemically. Not only do nucleic acid templates increase the... [Pg.279]

Some nucleic acid templates that direct synthesis could be considered enzymes, since they facilitate chemical reactions while remaining themselves unchanged. Most of the previously described reactions are dependent on base pairing between nucleic acid strands to facilitate proximity of reactants. Based on these observations... [Pg.281]

Biomacromolecules were exploited as templates in the enzymatic synthesis of electronic and photonic materials for the development of biofunctional complexes and selective biosensors. The aniline was polymerized by the catalysis of HRP on a calf thymus DNA matrix consisting of a synthetic oligonucleotide (poly[dA-dC].poly[dG-dT]) [54]. The complexation of PANI with DNA and the ohgonucleotide was found to induce reversible changes in the secondary structure of the nucleic acid template, leading to the formation of an overwound polymorph. The melting behavior of the PANI/DNA complex demon-... [Pg.81]


See other pages where Nucleic acid template is mentioned: [Pg.62]    [Pg.146]    [Pg.108]    [Pg.109]    [Pg.109]    [Pg.111]    [Pg.112]    [Pg.54]    [Pg.684]    [Pg.185]    [Pg.185]    [Pg.379]    [Pg.722]    [Pg.109]    [Pg.122]    [Pg.193]    [Pg.1014]    [Pg.174]    [Pg.117]    [Pg.151]    [Pg.567]    [Pg.1049]    [Pg.416]    [Pg.284]    [Pg.115]    [Pg.114]   
See also in sourсe #XX -- [ Pg.151 ]




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Nucleic Acid Templating Activity

Template Polymerization of Methacryloyl-Type Monomers Containing Pendant Nucleic Acid Bases

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