Murine cells

Morla, A. 0., Schreurs, J.,Miyajima, A., and Wang, J. H. J. (1988). Hematopoietic growth factors activate the tyrosine phosphorylation of distinct sets of proteins in interleukin-3-dependent murine cell lines. Mol. Cell. Biol. 8 2214-2218. 

Characterization of the exact nature of the post-translational modifications the mammary system is capable of undertaking. For example, the carbohydrate composition of tPA produced in this system differs from the recombinant enzyme produced in murine cell culture systems. 

Triacelluvax (combination vaccine containing r(modified) pertussis toxin Hepacare (r S, pre-S and pre-S2 hepatitis B surface antigens, produced in a mammalian (murine) cell line)... 

Vector Producer Cells. The direct administration of retroviral vector producer cells (e.g., murine cells producing HTK vector) to patients. 

Ford, J.M., Bruggemann, E.P., Pastan, I., Gottesman, M.M. and Hait, W.N. (1990) Cellular and biochemical characterization of thioxanthenes for reversal of multidrug resistance in human and murine cell lines. Cancer Research, 50, 1748-1756. 

The main attraction of stromal culture of hematopoietic cells is their superior ability for stem cell expansion especially in direct co-culture. However, despite this, a clinical use has not been realized until now. The major drawback is the origin of the stromal support. While autologous stroma would be feasible, in many cases this is not reaHzable. Furthermore, the use of cell Hnes (although irradiated to prevent further proliferation) is problematic as all stromal cells have to be removed completely prior to transplantation, a demand which is difficult to fulfill. In the case of murine cell Hnes a transfer of residue cells into a patient would be a xenotransplantation, which is faced with extensive regulatory hurdles. 

In the EST, two murine cell lines are used to assess teratogenic potential the embryonic D3 stem cell (ES) which represents the embryonic tissue and the 3T3 fibroblast cell which represents the adult tissue. The D3 cells are maintained in an undifferentiated stage in the presence of leukemia inhibiting factor (LIE), then released from LIE and allowed to form embryo bodies that differentiate into cardiomyocytes. The D3 and 3T3 cells are exposed to a range of concentrations of the test ingredient. After a 10-day... 

It is not known whether the transformation of Balb/c 3T3 cells is also a two-step process, like that in the other subtetraploid murine cell line, C3H lOTl/2. However, the results with the preneoplastic hamster cell line BHK are more consistent with a one-step process (Bouck and DiMayorca, 1976). 

Scherr, M. and Rossi, J.J. (1998) Rapid determination and quantitation of the accessibility to native RNAs by antisense oligodeoxynucleotides in murine cell extracts. Nucleic Acids Res., 26, 5079-5085. 

Karr JF, Kantor JA, Hand PH, Eggensperger DL, Schlom J. The presence of prostate-specific antigen-related genes in primates and the expression of recombinant human prostate-specific antigen in a transfected murine cell line. Cancer Res 1995 55 2455-2462. 

Godek ML, Duchsherer NL, McElwee Q, Grainger DW. Morphology and growth of murine cell lines on model biomaterials. Biomedical Sciences Instrumentation 2004, 40, 7-12. 

Another method to retain cells is to trap the cells inside a polymer matrix. For instance, mammalian cells were entrapped in PEG-based hydrogel fabricated in glass-Si chips (see Figure 8.19). The cells included murine 3T3 fibroblasts and SV-40- transformed murine cells (i.e., hepatocytes, macrophages). A cell adhesion... 

Mice and humans (and their respective cytokines and receptors) have been evolutionarily separated for over 100 million years. Consequently, the murine interferons differ from all of the human interferons at fifty-six to seventy-two amino-acid positions. Nevertheless, it was possible, simply by recombining human sequences, to produce an interferon with an activity greater than that of the natural mouse protein. Sequences that had been pre-tested in humans to function as a-interferons contained the information necessary to build a protein that functions well with murine cells. Even though it was not possible to reconstruct the exact sequence of murine a-interferon from the human genes, it was possible to reconstruct its function. 

Solberg H, Lober D, Eriksen J, Ploug M, Ronne E, Behrendt N, et al. Identification and characterization of the murine cell surface receptor for the urokinase-type plasminogen activator. Eur J Biochem 1992 205(2) 451 158. 

For immunization with receptor transfectants, 10-20 x 106 cells per mouse are needed. If the transfectants were established in a syngeneic murine cell line, they must be treated with mitomycin C to prevent tumor formation. 

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