Murine melanoma cell lines

Murine melanoma cell line B16F1 was obtained from ATCC (Manassas, VA, U.S.A.). 

The nm23 gene was found overexpressed in a nonmetasta-tic murine melanoma cell line but present in low levels in a highly metastatic ceU line. The nm23 gene product appears to be elevated in metastatic breast, colon, and prostatic cancer and is now being evaluated as a new marker for metastases. ... 

The use of liposomes as boron delivery vehicles for BNCT has been extensively studied, since liposomes can transport large amounts of boronated molecules, both hydrophobic and hydrophilic in nature, selectively into tumor cells. Three methods for incorporation of boron into liposomes have been reported incorporation of hydrophobic molecules into lipid bilayers, entrapment of hydrophilic molecules into the liposomes aqueous core, and the use of boronated lipids. Recently, PEG-based liposomes (DPPC/cholesterol/DSPC-PEG2000) have been used as effective carriers of boronated compounds within tumor tissues. Intraveuously injected B-PEG-liposomes on human pancreatic carcinoma xenografts in nude mice inhibited tumor growth after thermal neutron irradiation. The cationic liposome formulation (DOTAP/DOPC/DOPE) was used to deliver ortho-carboranyl P-lactoside (LCOB) aud l-methyl-ort/j< -carboranyl-2-hexylthioporphyrazine (H2PZ-COB) within DHD/K12/TRb (DHD) rat colon carcinoma and B16-F10 murine melanoma cell lines. Results from these studies show that a thirty-fold increase on the cellular uptake of B was obtained for the boronated compounds in the liposome formulation as compared with that of the BPA-fructose complex. 

In a similar way, sarcoma growth factors (SGFs e.g. ESG) can be obtained by the serum-free cultivation of murine sarcoma virus transformed 3T3 cells or transformed rat kidney cells. Nerve growth factor (NGF) is produced in excess by the human melanoma cell line A375 and fibroblast growth factor (FGF) is secreted by fibrosarcoma cells (Todaro et al., 1979). 

Fc-humanized mAbs are the result of genetic engineering to replace the murine Fc with human Fc in the melanoma cell lines [49]. 

Betulinic acid (21) Selective cytotoxic activity against melanoma cell lines antitumor effect in melanoma murine xenograft system 72... 

A variety of cell lines, including murine-F9 embryonal-carcinoma cells, murine-melanoma cells (B16 and S9), HeLa cells and HL-60 promyelocytic leukemia cells, have been successfully used for the extraction of RARs from nuclei (1,9). For cell lines growing as monolayers, we have used three 75-cm flasks at cell densities of 5 x 10 to 10 cells per flask for a typical experiment. In the case of HL-60 cells, which grow only in suspension culture, the cells are normally maintained at densities of 0.3 x 10 to 0.9 x 10 cells/mL but may be... 

Nitric oxide synthase activity is up-regulated in melanoma cell lines, and NO release was considered to encourage metastasis and tumor growth by maintaining the vasodilator tone in vessels around the melanoma (Joshi et al. 1996). Murine melanoma cells can be induced to express iNOS with a combination of cytokines and did not metastasize when the cells were injected into syngenic mice, whereas... 

The cytotoxicity of diploicin (77) was evaluated by Millot and coworkers in 2009 against B16 (murine melanoma) and HaCaT (human keratinocyte) cell lines [100]. Pannarin (66), a secondary metabolite from lichen Diploicia canescens, was tested by two different research groups for its inhibitory potential against DU-145 prostate carcinoma [100] and M14 (human melanoma) cell lines with positive results [96]. Recently, the cytotoxicity of proto-lichesterinic acid (8) against HeLa cell lines has been evaluated by Brisdelli and coworkers. Its activity is based on its abdity to induce cell death through activation of caspases-3, -8, and -9 [101]. 

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