Hydrogel fabrication

Another method to retain cells is to trap the cells inside a polymer matrix. For instance, mammalian cells were entrapped in PEG-based hydrogel fabricated in glass-Si chips (see Figure 8.19). The cells included murine 3T3 fibroblasts and SV-40- transformed murine cells (i.e., hepatocytes, macrophages). A cell adhesion... 

Representative natural materials used for hydrogel fabrication are fibrin, gelatin, collagen, cellulose, agarose, alginate, dextran, chitosan, and HA as well as other GAGs. 

Table 9.2 Comparison of gelation mechanisms for hydrogel fabrication...

T. Eoth, R. Henning, C. Kascholke, R. Hotzel, and M.C. Hacker, Reactive and stimuli-responsive maleic anhydride containing macromers-multifunctional cross-linkers and building blocks for hydrogel fabrication. React. Funct. Polym., 73,1480-1492, 2013. 

PI Lee. Synthetic hydrogels for drug delivery Preparation, characterization and release characteristics. In DST Hsieh, ed. Controlled Release Systems Fabrication Technology, Vol II. Boca Raton, FL CRC Press, 1988, pp 83-110. 

In contrast to high density arrays low density arrays are made by deposition of pre-synthesized oligonucleotides or proteins on activated surfaces. There are several printing techniques for fabricating microarrays Non-contact biochip arrayers, commonly based on the piezoelectric effect, can apply controlled sub-nanoliter probe volumes to pre-specified locations on the chip surface. Due to the fact that the dispenser does not touch the surface, a non-contact arrayer provides low risk of contamination and is most suitable for printing on soft materials such as hydrogels. 

Cell-Based Biochips Fabricated Using a PMBV/PVA Hydrogel. 157... 

The microfluidic chip system for preparing a miniaturized PMBV/PVA hydrogel consists of a two-chamber chip, an aluminum custom-made chip holder, Teflon capillaries, microtubes, and syringes equipped with a microsyringe pump (Fig. 15). The two-chamber chip was fabricated by a photolithographic wet etching technique. Whereas both channels and chambers (200 pm in depth) were fabricated on the top plate, only chambers (200 pm in depth) were fabricated on the bottom plate. 

Table VIII. HEMA hydrogel lenses fabricated from monomer mixes containing different initiators.

Worm end products, 18 646 Worsted wool-processing system, 26 383-384, 385-386 Worsted yarn, 11 178 Wort, 3 563, 564, 574, 575, 583 separation, 3 578-579 Wound closure, suture size and, 24 216 Wound closure biomaterials, 24 205. See also Sutures Wound dressings cotton smart, 3 31 ethylene oxide polymers in, 10 687 hydrogels in, 13 751-752 Woven fabrics, 11 178 dyeing, 9 170-171 Woven flax fibers, 11 594 Woven plastic bags, 18 12 Wovens... 

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