Hybridoma cell, murine

Figure 13.5 Outline of the production strategy of CEA-SCAN. The antibody-producing hybridoma cell line was originally obtained by standard methods of hybridoma generation. Spleen-derived murine B-lymphocytes were fused with murine myeloma calls. The resulting stable hybridomas were screened for the production of anti-CEA monoclonals. The clone chosen produces an IgG anti-CEA antibody. Note that the finished product outlined above is not radiolabelled. The freeze-dried antibody preparation (which has a shelf life of 2 years at 2-8 °C) is reconstituted immediately prior to its medical use. The reconstituting solution contains 99mTc, and is formulated to facilitate direct conjugation of the radiolabel to the antibody fragment...

Bhunia, A. K., Steele, P., Westbrook, D., Bly, L., Maloney, T., and Johnson, M. (1994). A six-hour in vitro virulence assay for Listeria monocytogenes using myeloma and hybridoma cells from murine and human sources. Microb. Pathog. 16, 99-110. 

Charbonneau JR, Gauthier ER (2000), Prolongation of murine hybridoma cell survival in stationary batch culture by Bcl-XL expression, Cytotechnology 34 131-139. 

Jang JD, Barford JP (2000), Effect of feed rate and antibody production in the fed-batch culture of murine hybridoma cells, Cytotechnology 32 229-242. 

Many inhibitors of catabolic pathways cause a decrease in cellular heat dissipation. They are therefore valuable tools to indicate the sources of the dissipation and give clues to the relative importance of each pathway in overall metabolic activity (see reviews by Kemp, 1987, 1993 Monti, 1987, 1991). To give a few examples from these reviews, sodium fluoride is a classical inhibitor of glycolysis and it has been shown to substantially reduce heat dissipation by human erythrocytes, lymphocytes, neutrophils, and murine macrophages, indicating the contribution of this pathway to metabolic activity. Cyanide inhibits oxidative phosphorylation by mitochondria at the cytochrome c oxidase complex (site 3) and studies revealed that it decreased heat production in a mouse LS-L929 fibroblast cell line but had no effect on human erythrocytes and neutrophils and murine macrophages, all of which lack mitochondria. Sodium azide inhibits at the same site and so it should come as no surprise that it had no effect on human neutrophils and lymphocytes, but it did reduce heat production by lymphocyte hybridoma cells, which contain... 

Thiophilic adsorption chromatography has been described for the purification of murine monoclonal antibodies from hybridoma cell culture containing fetal bovine serum.154 Due to the very low concentration of immunoglobulins in cell culture supernatants, binding capacity remains modest in spite of the presence of 0.5 to 1 M potassium sulfate. Further developments of this technology described by Nopper et al.155 indicated that thiophilic sorbents could be modified in their structure to increase the specificity and the binding capacity. 

Table 6.1.1 Categorization of viral contaminants of murine hybridoma cells ...

Most of the information on DNA rearrangements in switched cells is derived from analysis of B cells that became transformed after switching. The transformation had immortalized the switched phenotype. With few exceptions, switching within transformed cell lines of the B lineage is a rare event. It has been observed in murine pre-B cell lines [27-29], human pre-B and B lymphomas [30], the murine B cell lymphoma 1.29 [31] and in several murine myeloma and hybridoma cell lines (e.g. [32]). In general, the frequencies of switching in these lines do not reflect the switch frequencies in the corresponding B cells from which they are presumed to be derived. 

Kromenaker SJ, Srienc F. (1991) Cell-cycle dependent potein accumulation by producer and nonproducer murine hybridoma cell hues a population analysis. BiotedmoL Bioeng., 38 665-677. Kroner KH, Nissinen V. (1988) Dynamic filtration of microbial suspensions using an axially... 

Monoclonal antibody Murine hybridoma cell polyvinyl formal resins (biomass support particles) Immobilized in macroporous [112]... 

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