Murine melanoma cells

H5. Hearing, V. J., Law, L., Corti, A., Appella, E., and Blasi, F., Modulation of metastatic potential by cell surface urokinase of murine melanoma cells. Cancer (Philadelphia) 48, 1270-1278 (1988). 

Murine melanoma cell line B16F1 was obtained from ATCC (Manassas, VA, U.S.A.). 

Humphries MJ, Olden K, Yamada KM. A synthetic peptide from fibronectin inhibits experimental metastasis of murine melanoma cells. Science 1986 233(4762) 467-70. 

Taylor, D.D., Taylor, C.G., Jiang, C.G. and Black, PH. (1988) Characterization of plasma membrane shedding from murine melanoma cells. Int. J. Cancer 47, 629-635. 

The assay was originally described by Nicolson and Winkelhake (1975) in order to test the specific adhesion ability of B16 murine melanoma cells selected in vivo for lung-specific colonization (Fidler, 1973a). The test measures the ability of a tumor-cell suspension to bind to and aggregate a similar suspension of organ-derived host cells. The obtained results showed that such heterotypic aggregation occurred in a specific way, and it was related to the preferred organ site for implantation. 

Chambers, A. F. and Wilson, S. (1988). Use of neor B16-F1 murine melanoma cells to assess clonality of experimental metastases in the immune-deficient chick embryo. Clin. Exptl. Metastasis 6, 171-182. 

Humphries, M. J., Matsumoto, K., White, S. L. and Olden, K. (1986a). Ohgosac-charide modification by swainsonine treatment inhibits pulmonary colonization by B16-F10 murine melanoma cells. Proc. Natl. Acad. Sci. USA 83, 1752-1756. 

Rusciano, D., Lorenzoni, P. and Burger, M. M. (1993). Paracrine growth response as a major determinant in liver-specific colonization by in vivo selected B16 murine melanoma cells. Invasion Metastasis 13, 212-224. 

Watanabe, H., Nabi, I. R. and Raz, A. (1991b) The relationship between motility factor receptor internalization and the lung colonization capacity of murine melanoma cells. Cancer Res. 51, 2699-2705. 

The effect of a series of 4-phenyl-5-(2/-Y, 4 -X, or 4/-X-cinnamoyl)-l,3,4-thiadiazolium-2-phenylamine chlorides was evaluated on B16-F10 murine melanoma cells in vitro and the tumors resulting from implanted B16-F10 cells in C57BL/6 mice. These compounds differ from each other only at the cinnamoyl ring substituent (42, X=N02 43, X=OH 44, X=F 45, X=Y=F). Upon exposure of B16-F10 cells to MI-D (42), 44, and 45, all of them at the same micromolar concentration (50 iM) decreased the cell viability to 8, 50, and 22%, respectively, while compound 43 did not show any significant effect under the same conditions. However, doses as low as 10 iM MI-D were sufficient to impair the cell growth over 72 h, but for 44 and 45 the effect on... 

The nm23 gene was found overexpressed in a nonmetasta-tic murine melanoma cell line but present in low levels in a highly metastatic ceU line. The nm23 gene product appears to be elevated in metastatic breast, colon, and prostatic cancer and is now being evaluated as a new marker for metastases. ... 

Metastasis can be treated by the use of the tumor cells as the drug carrier. Doxorubicin-loaded B16-F10 murine melanoma cells were intravenously administered into C557B1 mice preinoculated with live B16-F10 tumor cells. The drug-loaded tumor cell system was able to prevent metastases formation from a control level of 554 in mice injected with saline solution to 4 in doxorubicin-treated mice. The drug-loaded cells were also able to cause remission of metastases after they were formed. 

Nojirimycin (247) inhibited protein synthesis and decreased the specific activity of y-glutamyltransferase in rat hepatoma cells [547]. It stimulated [5-glucosidase synthesis in Sporotrichum thermophile [548], and inhibited pulmonary colonization by B16-F10 murine melanoma cells [549],... 

Oxazofurin (512) was weakly cytotoxic toward B16 murine melanoma cells in culture, but inactive against P388 and L1210 murine leukemia and HL60 human leukemia (90JMC2849). It was also inactive against DNA... 

Abciximab (ReoPro) was the first partially humanized antibody approved for therapeutic use. It was produced from transgenic murine melanoma cells containing human heavy-chain genes so that the resulting Fab fragment is a chimera of human and mouse Ab fragments. Abciximab binds to the glycoprotein llb/llla... 

Several other assays of retinoid activity exist [83] and include evaluations of the ability of a retinoid to inhibit the exponential growth of S91 murine melanoma cells or to induce terminal differentiation of the fully neoplastic F9 teratocarcinoma cells. These and other assays of retinoid activity have been useful but the TOC, ODC, and HL-60 assays have been used most comprehensively in establishing retinoid structure-activity relationships, which are discussed below. 

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