Murine leukemia cell lines

Biochemical Studies Pironetin showed antitumor activity against a murine tumor cell line, P388 leukemia, transplanted in mice [4], and induced the... 

As described in several monographs [4], bryostatin 1 exhibits significant in vitro and in vivo antineoplastic activity against a range of tumor cell lines including murine leukemia, B-cell lymphoma, reticulum cell sarcoma, ovarian carcinoma, and melanoma. It is also effective in the modulation of apoptotic function [5], the reversal of multidrug resistance [6], and stimulation of the immune system [7]. These unique features displayed by bryostatin 1 are attributed to its high affinity for protein kinase C (PKC) isozymes and its ability to selectively modulate their functions [8]. PKCs are a type of intracellular serine and threonine kinase that... 

The related stannylpyrrole 90, which was reported by Dubac [72], has been used to synthesize the sponge metabolite mycalazol 11 (91) and related compounds, which have activity against the P388 murine leukemia cell line [74]. 

In 1991, Kojiri et al. reported the isolation of BE-13793C (344), an alkaloid isomeric with the arcyriaflavins C (347) and D (348) (see Scheme 2.89), from the culture broth of Streptoverticillium moharaense strain BA 13793 collected in Seto, Aichi Perfecture, Japan. BE-13793C (344) showed strong inhibitory activity against topoisomerases I and II and inhibited the growth of doxorubicin-resistant or vincristine-resistant P-388 murine leukemia cell lines, as well as their parent P-388 cell line (330). 

Results from testing against a variety of polymerase enzymes show that efavirenz is inactive up to 300 p,M for a 50% inhibition (Young et al., 1995). The polymerase enzymes studied were Moloney murine leukemia virus RT, human DNA polymerases a, 3, and 7, Escherichia coli RNA polymerase, and the Klenow fragment. Cytotoxicity studies in their primary cells and in a T-cell line reveal that efavirenz has a selectivity index of 80,000. 

In the EST, two murine cell lines are used to assess teratogenic potential the embryonic D3 stem cell (ES) which represents the embryonic tissue and the 3T3 fibroblast cell which represents the adult tissue. The D3 cells are maintained in an undifferentiated stage in the presence of leukemia inhibiting factor (LIE), then released from LIE and allowed to form embryo bodies that differentiate into cardiomyocytes. The D3 and 3T3 cells are exposed to a range of concentrations of the test ingredient. After a 10-day... 

Preliminary in vitro and animal studies of the effects of silymarin and silybinin have been carried out with several cancer cell lines. In murine models of skin cancer, silybinin and silymarin were said to reduce tumor initiation and promotion. Induction of apoptosis has also been reported using silymarin in a variety of malignant human cell lines (eg, melanoma, prostate, leukemia cells, bladder transitional-cell papilloma cells, and hepatoma cells). Inhibition of cell growth and proliferation by inducing a Gx cell cycle arrest has also been claimed in cultured human breast and prostate cancer cell lines. The use of milk thistle in the clinical treatment of cancer has not yet been adequately studied but preliminary trials are under way. 

Discorhabdins A (8), B (9) and C (10) have been isolated from several sponge species of the genus Latrunculia from New Zealand. They are powerful cytotoxins with IC50 values against the murine leukemia P-388 cell line in the range 0.03 - 0.01 pg/ml. Discorhabdin A has also been isolated from a Japanese sponge of the genus Prianos [29]. 

Lewis, S., Rosenberg, N., Alt, F., Baltimore, D. (1982). Continuing kappa-gene rearrangement in a cell line transformed by Abelson murine leukemia virus. Cell 30, 807-816. 

The title substituted 6 phenylpurine bases and nucleosides 10—13, as well as some acetyl derivatives 8, were tested on their in vitro inhibition of the cell growth in the following cell cultures mouse leukemia L1210 cells (ATCC CCL 219) murine L929 cells (ATCC CCL 1) human cervix carcinoma HeLa S3 cells (ATCC CCL 2.2) and human T lymphoblastoid CCRF-CEM cell line (ATCC CCL 119). Only substituted 6-phenylpurine ribonucleosides 12 and their triacetates 8 exhibited significant activity in these assays (Table 1), while the bases 10 and 11, as well as the 2 amino 6 phenylpurine ribonucleosides 13, were entirely inactive. 

The antiproliferative activity of 1,2,3-dithiazoles 168-176 has been tested in vitro on human myeloid leukemia K562 and L1210 murine leukemia cell lines and compared to the antiproliferative effects of the natural product distamycin A. All the imines 168-176 are active at concentration ranging from 3 to 10 pM and they retain antiproliferative activities comparable to those exhibited by distamycin A. 

Among the alkaloids, the most unusual example is an acaricidal (lethal to arachnids) monot-erpene derivative, altemicidin (Structure 18.3). This novel alkaloid was purified from amarine strain of Streptomyces sioyaensis SA-1758 isolated from marine sediments collected from the northern part of Japan. It yielded potent antitumor activity in vitro against L1210 murine leukemia and IMC carcinoma cell lines, but was toxic in vivo in mice. Altemicidin is a novel sulfur- and nitrogen-containing microbial metabolite with a monoterpene carbon skeleton.12... 

The antineoplastic activity of vanadium compounds has been studied for some time. In 1979, the metalocene compound, biscyclopentadienyldichloro-Vanadium(IV), (C5H5)VCI2, was found to have antitumor activity [161], The compound inhibited the growth of various cancer cell lines and the growth of solid tumors in vivo. Vanadium(V) peroxocomplexes with known insulin-mimetic activity were shown to have antitumor activity against murine leukemia cells at that time. Vanadocene compounds are now known to induce apoptosis in cell lines. The apoptotic signal... 

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