Murine monocyte/macrophage cell line

In the first differential proteomics approach for the identification and characterization of proteins involved in both the inflammatory cascade and Leflunomide mode of action (Dax et al, 1998), the murine monocyte/macrophage cell line RAW 264.7 was employed and the RAW 264.7 proteomes of normal, unstimulated RAW 264.7 macrophages with the respective LPS-stimulated and/or drug-treated were compared. This cell line was chosen since RAW 264.7 cells have been used as a model system to study immune responses (Bahl et al, 1994) successfully before. 

A murine monocytes-macrophages cell line was used as a model to study foreign-body response and phagocytosis capability. The adhesion and proliferation of these cells increased after alkaline surface hydrolysis of P3HB-8%3HV films. Pretreatment of the unmodified polymer with collagen had a repulsive effect on the cells, which disappeared on the hydrolyzed polymer, while fibronectin promoted cell adhesion on both siu-faces [122]. 

TNF is a macrophage/monocyte-derived anticancer cytokine (Carswell et al. 1975, Matthews 1978, Niitsu et al. 1985) with strong cytotoxicity to tumour cells in vitro (Helson et al. 1975, Niitsu et al. 1988, Watanabe et al. 1985, 1988). In the transformed murine macrophage cell line RAW 264.7, TNF-a up-regulation 4h after lipopotysaccharide addition seems to involve protein kinase C, as stau-rosporine strongly inhibited (over 70 %) the expression of TNF-a mRNA while calphostin had no significant influence (Tremblay et al. 1995). 

Extracellular reduced glutathione could completely attenuate the cristobalite (particle diameter 1.2 on)-induced expression of monocyte chemo-tactic protein-1 and macrophage inflammatory protein-2 mRNAs by a murine alveolar type II line, whereas TNF-a mRNA levels were unaltered (Barrett et al. 1999). Using the oxidant sensitive dye, 6-carboxyl-27 -dichlorodihydrofluorescein diacetate di(acetoxymethyl ester) treatment of this cell line with cristobalite (18 ftg/cnf = 100 pg/ial) and TNF-a (1 ng/ml) resulted in the production of reactive oxygen species, which could be inhibited with extracellular GSH treatment. In the case of cristo-balite-induced reactive oxygen species, inhibition was also achieved with an anti-TNF-a antibody. 

TIB 71 RAW 264.7 Murine DM10 A phagocytic monocyte/macrophage line capable of antibody-dependent lysis of target cells... 

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