Micronucleus test in vivo

TETA was mutagenic in bacterial assays and was positive in sister chromatid exchanges and unscheduled DNA synthesis tests in vitro It was not clastogenic in the mouse micronucleus test in vivo after oral or intraperitoneal administration. 

Aeschbacher, H. U., H. Meier, E. Ruch, and H. P. Wurzner. Investigation of coffee in sister chromatid exchange and micronucleus tests in vivo. Food Chem Toxicol 1984 22(10) 803-807. 

Micronucleus test in vivo MNT in vivo Chromosome aberrations Induction of micronuclei... 

Micronucleus test in vivo Mice Pyrogallol resorcinol Mitra and Manna (1977) Gocke et al. (1981)... 

Physicochemical properties requked include melting/boiling point, vapor pressure, solubiUty, and flammabiUty/explosion characteristics. The toxicological studies include acute toxicity tests, oral, inhalation, and dermal skin and eye kritation skin sensiti2ation subacute toxicity, oral, inhalation, and dermal and mutagenicity tests. In vitro reverse mutation assay (Ames test) on Salmonella typhimurium and/or E.scherichia coli and mammalian cytogenic test. In vivo mouse micronucleus test. 

Miller BM, Pujadas E, Gocke E. 1995. Evaluation of the micronucleus test in vitro using Chinese hamster cells Results of four chemicals weakly positive in the in vivo micronucleus test. Environ Mol Mutagen 26 240-247. 

Fritzenschaf, H., Kohlpoth, M., Rusche, B. Schiffmann, D. (1993) Testing of known carcinogens and noncarcinogens in the Syrian hamster embryo (SHE) micronucleus test in vitro correlations with in vivo micronucleus formation and cell transformation. Mutat. Res., 319, 47-53... 

In mammalian assays, glycidol has been tested in human lymphocytes and Chinese hamster cells in vitro for induction of chromosomal aberrations and sister chromatid exchanges. It was also tested in vivo in the mouse micronucleus assay. All test results were positive, as were those of gene mutation assays using Chinese hamster V79 cells and mouse lymphoma L5178Y cells. An in-vivo assay to detect chromosomal aberrations in mouse bone-marrow cells gave negative results. 

Van Hummelen, P., Zoll, C., Paulussen, J., Kirsch-Volders, M. and Jaylet, A. (1989) The micronucleus test in Xenopus a new and simple in vivo technique for detection of mutagens in fresh water, Mutagenesis 4, 12-16. 

Developmental rats, rabbits Prenatal and postnatal development none Genetic toxicology Ames test, in vitro mammalian chromosome aberration test in V79 Chinese hamster cells, and in vivo mammalian erythrocyte micronucleus test in rats... 

Developmental rats, rabbits Prenatal and postnatal development rats Genetic toxicology Ames tests, mouse lymphoma cell forward gene mutation test, human peripheral blood lymphocyte chromosome aberration test, in vivo micronucleus test in mice and ex vivo UDS test in rat liver hepatocytes... 

Not genotoxic (Ames, human lymphocyte peripheral blood lymphocyte chromosome aberration test, in vivo mouse micronucleus test)... 

Genetic toxicology Ames test, in vivo mouse micronucleus... 

Chromosomal aberration tests in vitro (e.g. Cytogenetic test) and in vivo (e.g. Micronucleus test in rodents). 

Mouse lymphoma test in mouse lymphoma cells (in vitro) Micronucleus test in rodent bone marrow (in vivo)... 

Benning, V, Depasse, E, Melcion, C., and Cordier, A. (1992). Detection of micronuclei after exposure to mitomycin C, cyclophosphamide and diethylnitrosamine by the in vivo micronucleus test in mouse splenocytes. Mutat Res 280, 137-142. 

Damage induced in whole animals can be detected in in vivo chromosome assays in either somatic or germinal cells by examination of metaphases or the formation of micronuclei. The micronucleus test can also detect whole chromosome loss or aneuploidy in the absence of clastogenic activity and is considered comparable in sensitivity to chromosome analysis (Tsuchimoto and Matter, 1979). 

Tsuchimoto, T. and Matter, B.E. (1979). In vivo cytogenetic screening methods for mutagens with special reference to the micronucleus test. Arch. Toxicol. 42 239-248. 

The in vivo micronucleus test is used for the detection of damage to chromosomes as well as the mitotic apparatus in bone marrow or peripheral blood cells of rodents. The assay system has been well standardized.14-17 The basic features of the test system are (1) the effect of the test chemical is observed in anucleated polychromatic erythrocytes (PCEs) (2) PCEs have a relatively short lifespan, so that any micronuclei they contain must have been generated as a result of recently induced chromosome damage (3) micronuclei are readily identifiable and their distribution is well defined and (4) the frequency of induced micronuclei in PCEs is dependent on sampling times. 

B.ll Mutagenicity - In vivo mammalian bone-marrow chromosome aberration test B.12 Mutagenicity mammalian erythrocyte micronucleus test B.20 Sex-Unked recessive lethal test in Drosophila melanogaster B.22 Rodent dominant lethal test... 

The mammalian in vivo erythrocyte micronucleus test OECD TG 474 US-EPA OPPTS 870.5395 EU Annex V B.12... 

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