Cells Chinese hamster

Bredinin, Neosidomycin, and SF-2140. Bredinin (62), isolated from the culture filtrates of Eupenicillium brefeldianum (1,4), inhibits the multiplication of L5178Y, HeLa S3, RK-13, mouse L-ceUs, and Chinese hamster cells. GMP can reverse the inhibition by (62), but (62) is not incorporated into the nucleic acids. The inhibition of nucleic acid synthesis and chromosomal damage in the S and G 2 phases that is caused by (62), is reversed by GMP. It blocks the conversion of IMP to XMP and XMP to GMP. In combination with GMP, (62) interferes with intracellular cAMP levels and thereby inhibits cell division. 

Landry, J., Chretien, P., Laszio, A., Lambert, H. (1991). Phosphorylation of hsp27 during development and decay of thermotolerance in Chinese hamster cells. J. Cell Physiol. 147,93-101. 

Westra, A. Dewey, W.C. (1971). Variation in the sensitivity to heat shock during the cell cycle of Chinese hamster cells in vitro. Inti. J. Radiat. Biol. 19,461-417. 

Chen HH, Sirianni SR, Huang CC. 1982. Sister chromatid exchanges in Chinese hamster cells treated with seventeen organophosphorus compounds in the presence of a metabolic activation system. Environ Mutagen 4 621-624. 

Sirianni SR, Huang CC. 1980. Comparison of induction of sister chromatid exchange, 8-azaguanine- and ouabain-resistant mutants by cyclophosphamide, ifosfamide and l-(pyridyl-3)-3,3-dimethyltriazene in Chinese hamster cells cultured in diffusion chambers in mice. Carcinogenesis 1 353-355. 

Romert, L. and Jenssen, D. (1983). Rabbit alveolar macrophage-mediated mutagenesis of polycyclic aromatic hydrocarbons in V79 Chinese hamster cells. Mutat. Res. Ill, 245-252. 

In mammalian test systems in vitro (Syrian or Chinese hamster cells), lead acetate gave conflicting results for structural chromosomal aberrations (Bauchinger and Schmid 1972 Robison et al. 1984). Lead... 

Another potential source of iron, at least for hepatocytes, is receptor-independent uptake of iron from transferrin. This appears to involve an iron uptake pathway from transferrin which is neither suppressed in hepatocytes by antibodies to TfR (Trinder et at, 1988), nor by transfection of HuH-7 hepatoma cells with transferrin receptor anti-sense cDNA (Trinder etat, 1996). The same pathway may also be utilized for iron uptake from isolated transferrin N-lobe, which is not recognized by the receptor (Thorstensen et at, 1995). The possible role of TfR2 in this process remains to be established, as does the physiological importance of this pathway in intact liver. Human melanoma cells (Richardson and Baker, 1994) and Chinese hamster cells lacking transferrin receptors but transfected with melanotransferrin (Kennard et at, 1995) use another pathway for transferrin iron uptake, independent of the transferrin receptor, but utilizing iron transfer from transferrin or simple iron chelates to membrane-anchored melanotransferrin, and from there onwards into the cellular interior. 

Murphy, F.R., Jorgensen, F.D., and Cantor, C.R. (1982) Kinetics of histone endocytosis in Chinese hamster cells. A flow cytofluorometric analysis./. Biol. Chem. 257, 1895. 

An increased frequency of sister chromatid exchanges was obtained in vivo in bone-marrow cells of male Swiss mice at intraperitoneal doses of 210 and 420 mg/kg (Parodi et al. 1982, 1983) and in vitro Chinese hamster cells (Abe and Sasaki 1977), although in the latter study, no chromosomal aberrations were observed. 

Abe, S., and M. Sasaki. 1977. Chromosome aberrations and sister chromatid exchanges in Chinese hamster cells exposed to various chemicals. J. Natl. Cancer Inst. 58 1635-1641. 

DMT, TMT, DBT, TBT and DPhT chlorides exhibited in vitro spindle disturbance in V79 Chinese hamster cells of brain tubulin. The V79 cells lose stainable spindles at higher concentration. The cell mitosis activity effect at low concentration increased with the lipophilicity of the OTC, but all compounds showed a concentration dependence on microtubules. The OTC seem to act through two different cooperative mechanisms inhibition of microtubule assembly and interaction with hydrophobic sites. The latter mechanism might involve Cl/OH ion exchange28. 

Lee, Y.W., C. Pons, D.M. Tummolo, C.B. Klein, T.G. Rossman, and N.T. Christie. 1993. Mutagenicity of soluble and insoluble nickel compounds at the gpt locus in G12 Chinese hamster cells. Environ. Molec. Mutagen. 21 365-371. 

Sofuni, T. and M. Ishidate, Jr. 1988. Induction of chromosomal aberrations in active oxygen-generating systems. I. Effects of paraquat in Chinese hamster cells in culture. Mutation Res. 197 127-132. 

Jansson, K, and V. Jansson. 1991. Induction of mutation in V79 Chinese hamster cells by tetrahydroquinone, a metabolite of pentachlorophenol. Mutat. Res. 260 83-87. 

Sister chromatide exchanges in Chinese hamster cells [261, 440] and in cultured human lymphocytes [441-443] have been found after nickel salt addition. 

Chinese Hamster Lines. Chinese hamster cell lines have given much valuable data over the past 15 years but their use for screening is limited by lack of sensitivity, because only a relatively small target cell population can be used, owing to metabolic co-operation (see Cole et al., 1990) however, they are still in use, so a brief description follows. 

Established cell lines, cell strains or primary cell cultures may be used. The most often used are Chinese hamster cell lines and human peripheral blood lymphocytes. The merits of these two cell lines have been reported (Ishidate and Hamois, 1987 Kirkland and Gamer, 1987). The cell system must be validated and consistently sensitive to known clastogens. 

Bowden, G.T., Hsu, I.C., and Harris, C.C. (1979). The effect of caffeine on cytotoxicity, mutagenesis and sister chromatid exchanges in Chinese hamster cells treated with dihydrodiol epoxide derivatives of benzo(a)pyrene. Mutation Res. 63 361-370. 

Chu, E.H.Y. and Mailing, H.U. (1968). Mammalian cell genetics. II. Chemical induction of specific lucus mutations in Chinese hamster cells in vitro. Proc. Nat. Acad. Sci. U.S.A. 61 1306-1312. 

Connell, J.R. and Medcalf, A.S. (1982). The induction of SCE and chromosomal aberrations with relation to specific base methylation of DNA in Chinese hamster cells by N-methyl-n-nitrosourea and dimethyl sulphate. Carcinogenesis 3 385-390. 

Ford, D.K. and Yerganian, G. (1958). Observations on the chromosomes of Chinese hamster cells in tissue culture. J. Nat. Cancer Inst. 21 393-425. 

Bale SS. 1983. Cytological effects of Kepone on Chinese hamster cells. J Hered 74(2) 123-124. 

V79 Chinese hamster cells Gene mutation + - Paschin and Bahitova 1982... 

Miller BM, Pujadas E, Gocke E. 1995. Evaluation of the micronucleus test in vitro using Chinese hamster cells Results of four chemicals weakly positive in the in vivo micronucleus test. Environ Mol Mutagen 26 240-247. 

Paschin YV, Bahitova LM. 1982. Mutagenicity of benzo[a]pyrene and the antioxidant phenol at the HGPRT locus of V79 Chinese hamster cells. Mutat Res 104 389-393. 

Hagman and Sivertsson discussed the work performed at Pharmacia and Upjohn52 on monitoring and controlling bioprocesses. They followed the protein production-derived form Chinese hamster cells (CHO-cells) in a 500-1 reactor over a 3-month period. The diagrams of the flow cell and pumping/NIR system are displayed, and the logic behind the work outlined. External and on-line calibrations were performed. 

Tezuka H, Ando N, Suzuki R, et al. 1980. Sister chromatid exchanges and chromosomal aberrations in cultured Chinese hamster cells treated with pesticides positive in microbial reversion assays. Mutat Res 78 177-191. 

Kurata M, Hirose K, Umeda M. 1982. Inhibition of metabolic cooperation in Chinese hamster cells by organochlorine pesticides. Gann 73 217-221. 

Genotoxic Effects. No studies were located regarding the genotoxicity of 1,2-diphenylhydrazine in humans by any route of exposure. A limited number of assays have been conducted using bacteria, mammalian cell and whole animal systems. As indicated in Table 2-2, 1,2-diphenylhydrazine was mutagenic in Salmonella typhimurium, out not in Escherichia coli, and produced chromosome aberrations and sister chromatid exchanges in Chinese hamster cells. An exogenous metabolic activation system was necessary for expression of the aforementioned effects. In in vivo studies... 

---