Cell line keratinocyte

Fig. 14 Effect of the molecular weight of tamarind seed xyloglucan depolymerized by ( ) 7-irradiation, ( ) ultrasonication, and ( ) endo-glucanase treatment on the production of various cytokines (Tumor necrosis factor a, TNE-a Interleukin 8, IL-8 Interleukin 10, IL-10 and Interleukin 12, IL-12) in HaCaT cells (Immortalized keratinocytes line) [301]...

Van Och, F.M. et al., Assessment of potency of allergenic activity of low molecular weight compounds based on IL-1 alpha and IL-18 production by a murine and human keratinocyte cell line, Toxicology, 210, 95, 2005. 

Gupta, A., Rosenberger, S. F., and Bowden, G. T., Increased ROS levels contribute to elevated transcription factor and MAP kinase activities in malignantly progressed mouse keratinocyte cell lines, Carcinogenesis, 20, 2063, 1999. 

Boukamp, P., R. T. Petrussevska, D. Breitkreutz, J. Homung, A. Markham, and N. E. Fusenig. 1988. Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line. J Cell Biol 106(3) 761-71. 

Several cell lines were used to inveshgate the role of PS oxidahon in apoptosis. Preferenhal oxidahon of PS was observed in human leukemia HL-60 cells (Fabisiak et al, 1998, 2000 Kawai et al, 2000), and normal human keratinocytes (Shvedova et al, 2001). Similarly, in pheochromocytoma PC 12 cells exposed to a radical-generating anhneoplashc dmg, neocarzinostatin, extemalizahon of PS was potentiated by its selechve oxidation in whole cells (Schor et al, 1999). In contrast, this selechve PS oxidahon did not occur in liposomes prepared from mixtures of PnA-labeled phospholipids extracted from the ceUs and exposed to oxidants imder the same conditions (Fabisiak et al, 1998 Kagan et al, 2000 Shvedova et al,... 

Particles from cationic lipids may also be useful for antisense therapy of skin disease — a nontoxic increase in the oligonucleotide uptake by cultivated keratinocytes and a sebocyte cell line has been reported [66]. Moreover, cationic dendri-mers also efficiently transfer reporter gene DNA to human keratinocytes cultivated in vitro. In the skin of hairless mice, in vivo transfection was possible with complexes, yet reporter gene expression was localized to perifollicular areas. Transfection, however, failed with the naked plasmid. For prolonged contact, biodegradable membranes coated with dendrimer/DNA complexes were used [67]. This hints at a follicular uptake of these complexes and indicates that gene transfection also may be possible with human skin, which has a thicker stratum comeum compared with mouse skin (eight to ten vs. two to three layers [58]). 

O Driscoll KR, Madden PV, Christiansen KM, Viage A, Slaga T), Fabbro D, Powell CT. Weinstein IB (1994) Overexpression of protein kinase C beta I in a murine keratinocyte cell line produces effects on cellular growth, morphology and differentiation. Cancer Lett 83 249-259... 

Benzoyl peroxide generally inhibits gap-junctional intercellular communication in cultured cells. In contrast, an increase in gap-junctional intercellular communication was observed in a Syrian hamster embryo cell line. Changes in the expression of gap-junctional proteins (connexins) concomitant with inhibition of gap-junctional intercellular communication have been observed. In SEN mice treated with 83 jmol benzoyl peroxide, keratinocytes expressed the gap-jimctional connexin 26 gene (not normally expressed in adult mouse skin), transiently increased the expression of connexin 43 and reduced the expression of connexin 31.1 (Budunova et al., 1995, 1996). In primary mouse kerati-nocyte cultures, benzoyl peroxide strongly decreased the amount of E-cadherin protein (Jansen etal., 1996). 

SERCA2a is expressed predominantly in the heart, in slow twitch skeletal muscle and in the brain, where it represents the main SERCA isoform. In contrast, SERCA2b is a house-keeping isoform, ubiquitously expressed in smooth muscle and non-muscle tissue (Wuytack et al., 2002). Although both isoforms are detectable in keratinocytes and dermal fibroblasts in culture, SERCA2b is the major isoform expressed in the epidermis from adult skin sections. SERCA2c is expressed in epithelial, mesenchymal and hematopoietic cell lines, and in monocytes (Table 1). 

Viruses can induce apoptosis of human keratinocytes. These apoptotic cells can express viral antigens and autoantigens, which are coclustered in specific subsets within surface blebs and then cause challenge to self-tolerance if not cleared and processed properly (R15). Andreassen et al. showed that T cell lines specific for polyomavirus T antigen recognize T antigen complexed with nucleosomes and trigger B cells to produce anti-DNA antibodies (A20). 

Hudson LG, Toscano WA Jr, Greenlee WF. 1985. Regulation of epidermal growth factor binding in human keratinocyte cell line by 2,3,7,8-tetrachlorodibenzo-p-dioxin. Toxicol Appl Pharmacol 77 251-259. 

Twenty years ago, a high-affinity-binding site for uPA was demonstrated on the surface of peripheral blood monocytes and cultured cells of the human histiocytic lymphoma cell line, U937 [46]. The expression of uPAR on the cell surface of many cell types has since then been demonstrated, including a variety of neoplastic cell lines as well as nonneoplastic cells such as neutrophils, macrophages, keratinocytes, placental trophoblasts, endothelial, and smooth muscle cells [7, 33, 47-51]. The human uPAR gene has been mapped to chromosome 19ql.3 [52]. 

Desferrioxamine and several related iron chelators have been demonstrated to inhibit the proliferation of a variety of malignant cell lines [148,149] as well proving inhibitory in acute neonatal leukaemia [150]. In addition desferrioxamine also exhibits in vivo anti-malarial activity in both humans and rats [151,152], Iron chelators have also been used to treat the inflammatory skin disorder, psoriasis, where there is hyperproliferation of keratinocytes and T-lymphocytes [153,154], A general problem still to be overcome with a number of powerful iron chelators is their lack of cell specificity and thus general toxicity for example to bone marrow function [144],... 

C23. Culig, Z., Hobisch, A., Cronauer, M. V., Radmayr, C., Trapman, J., et al, Androgen receptor activation in prostatic tumor cell lines by insulin-like growth factor-1, keratinocyte growth factor and epidermal growth factor. Cancer Res. 54,5474—5478 (1995). 

As has been discussed, 1,25-(OH)2D3 carries out a broad range of actions in many nonclassical target tissues, including immune cells and several leukaemia cell lines in addition to its central role in bone and calcium metabolism. Also, 1,25-(OH)2D3 has been demonstrated to have important general effects on cell proliferation and differentiation including cancer cells, epidermal keratinocytes, and activated lymphocytes. 

Doxycycline inhibits the detachment of SM-exposed HaCaT cells in culture from the growth substrate. However, analysis of the metabolic activity of the adherent cells reveals that doxycycline treatment does not maintain cell viability. It is suggested that doxycycline and other matrix metalloprotease inhibitors may have a role to play in therapeutic intervention against SM, only as a combination therapy (Lindsay et al, 2007). Human keratinocyte cell lines were pretreated with mixtures of methenamine and glutathione prior to SM exposure. Though it is possible to protect the cell cultures from the toxic effects of SM, it will be effective only as a pretreatment (Smith et al, 1997). 

Arya and Dandia [26] presented the HY zeolite promoted, environmentally benign solvent free synthesis of 2,4,6-trisubstituted-l,3,5-triazines (xiv) under microwave irradiation. The synthesized derivatives also showed promising activities when screened for phototoxicity and cytotoxic activities against leukemia and adenocarcinoma derived cell lines in comparison to the normal human keratinocytes. 

Immortalized mammalian cell lines such as HeLa, V79, human keratinocytes and mouse fibroblasts and canine kidney cells to study... 

TNFa is produced primarily by activated monocytes and/or macrophages and, to a lesser extent, by many other types of cells, such as activated T cells (Thi celts), B cells, NK cells, mast cells, endothelial cells, fibroblasts, keratinocytes, microglia, astrocytes, Kupffer s cells, smooth musde cells, synovial lining cells, and basophils (Figure 22-29). ... 

Extensive reviews concerning the opportunities for the development of in vitro sensitization methods already exist [41-44], These reviews show that essentially all of the methods address one or other of the key mechanistic steps in the induction of skin sensitization—and these are nicely represented in the OECD adverse outcome pathway description [45], From this large body of work, three methods have emerged whose initial promise has been substantiated by demonstration not only of their predictive merits but also by verification of their robustness in terms of inter-laboratory transferability and within and between laboratory reproducibility [46]. The three methods are the direct peptide reactivity assay (DPRA) [47, 48], KeratinoSens [49, 50], and the human Cell Line Activation Test (h-CLAT) [51-53]. The first of these, the DPRA, addresses the question of chemical reactivity, the second investigates an aspect of keratinocyte activation... 

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