HaCaT cells

The tamarind seed XG exerts several biological activities, such as marked inhibitory effect on the binding of BK virus to cells [299] and immunomodulatory effects [300]. Recently, it was reported [301] that XG affects the proliferation of cytokines in various skin-cell lines (Fig. 14) such as HaCaT cells (im-... 

Fig. 14 Effect of the molecular weight of tamarind seed xyloglucan depolymerized by ( ) 7-irradiation, ( ) ultrasonication, and ( ) endo-glucanase treatment on the production of various cytokines (Tumor necrosis factor a, TNE-a Interleukin 8, IL-8 Interleukin 10, IL-10 and Interleukin 12, IL-12) in HaCaT cells (Immortalized keratinocytes line) [301]...

Fig. 15.21 Peak type detection of HaCaT cells settling onto the sensor surface, (a) Three sensorgrams obtained after 40, 90 and 200 min (b) angular position of the peak vs. time and (c) microscope image of the exposed sensor surface taken right after the measurements. The area shown is 460 x 400 pm2. Reprinted from Ref. 27 with permission. 2008 Elsevier...

Wieder, T., PerUtz, C., Wieprecht, M., Huang, R.T., Geilenm C.C. and Orfanosm, CR., 1995, Two new sphingomyelin analogs inhibit phosphatidylcholine biosynthesis by decreasing membrane-bound CTP phosphocholine cytidylyltransferase levels in HaCaT cells. Biochem. J. 311 873-879... 

Using human keratinocytes (HaCaT cells) to determine the direct interaction of purified BC with cells in culture, good biocompatibility of BC with cells was shown, with no cytotoxicity effects. 

Small water-soluble molecules can also be introduced into cells via stimulation of fluid-phase uptake by the described protocol. Uptake of Lucifer Yellow (m.w. 457.2 Lucifer Yellow CH, dilithium salt from Sigma Chemicals, Rehovot, Israel) has been stimulated by 2.2-fold and 2.7-fold in COS 5-7 and HaCaT cell lines, respectively, as compared with the constitutive uptake. 

Fig. 3. Activity of HRP following LEF induced uptake into COS 5-7 and HaCaT cells. Cells (2 x 106/mL) were exposed to LEF (20 V/cm, 500 Hz, and pulse width 180 (is) for 1 min in the presence of 1 mg/mL HRP in DMEM-H medium. Three hours after exposure, the cells were washed three times in PBS and disrupted by five freeze-thaw cycles in 50 (jlL PBS containing anti-protease cocktail (1 100). Dot-blot analysis of HRP activity 1 - cells without HRP 2 - non-exposed cells with HRP 3 and 4 -duplicates of cells exposed to LEF in the presence of HRP.

Lindsay, C.D., Gentilhomme, E., Mathieu, J.D. (2007). The use of doxycycline as a protectant against sulphur mustard in HaCaT cells. J. Appl. Toxicol. 28 665-73. 

Doxycycline inhibits the detachment of SM-exposed HaCaT cells in culture from the growth substrate. However, analysis of the metabolic activity of the adherent cells reveals that doxycycline treatment does not maintain cell viability. It is suggested that doxycycline and other matrix metalloprotease inhibitors may have a role to play in therapeutic intervention against SM, only as a combination therapy (Lindsay et al, 2007). Human keratinocyte cell lines were pretreated with mixtures of methenamine and glutathione prior to SM exposure. Though it is possible to protect the cell cultures from the toxic effects of SM, it will be effective only as a pretreatment (Smith et al, 1997). 

Kehe, K., Raithel, K., Kreppel, H., Jochum, M., Worek, F., Thiermann, H. (2007). Inhibition of poly(ADP-rihose) polymerase (PARP) influences the mode of sulfur mustard (SM)-induced cell death in HaCaT cells. Arch. Toxicol. 82 461-70. 

Ledirac N, Delesculse C, de Sousa G, Pralavorio M, Lesca P, et al. 1997. Car-baryl induces CYP1A1 gene expression in HepG2 and HaCaT cells but is not a ligand of the human hepatic Ah receptor. Toxicol. Appl. Pharmacol. 144 177—82... 

Fig. 4.6 Cellular localization of FAAFI in human keratinocytes. Co-localization of FAAFI with calnexin (marker to endoplasmic reticulum). Human keratinocytes (HaCaT cells) were co-stained with anti-FAAH (in green) and anti-calnexin (in red) antibodies. Superimposition of the two stainings (merge) revealed a vesicular region of the endoplasmic reticulum where FAAH and calnexin largely overlapped (yellow). Dot structures, where FAAH and calnexin co-localized, are indicated by the white arrows in the inset at the bottom of the merge panel. The remaining part of the reticulum, with lamellar appearence, did not display any colocalization of the two proteins. Courtesy of Dr. Sergio Oddi (University of Teramo, Italy)...

Reeently, Saliou and coworkers have reported that oral supplementation of Pyenogenol reduees UV-induced erythema in the hitman skin and also significant inhibits the aetivatiorr, the binding to DNA, and the NF-rcB-dependent gene expression in the immortalized human keratinoeyte cell line HaCaT. In the same eontext, Pycnogenol has been observed to proteet GSH levels and to partially suppress the cytotoxicity induced by UV treatment, both in human primary keratinoeytes and in HaCaT cells. ... 

Fig. 5 C-terminal Cdk-binding domain of p21 conjugated with antennapedia PTD suppresses pRb phosphorylation and induces Gj/S cell cycle arrest. (A) Peptide sequences of fusion polypeptides composed of C-terminal Cdk-binding domain of p21 (p21141.160 [peptide I] or p21i54.i6o [peptide II]) and antennapedia PTD boxed). (B) pRb became hyperphosphorylated between 12 and 15 h after serum was added to starved HaCat cells, but in the presence of peptide I or II remained hypophosphorylated. pRb = hypophosphorylated, pRb = hyperphosphorylated. (C) Cell cycle distribution of HaCat cells after culture in D-MEM medium containing 10% PCS alone or 10% PCS with peptide I or II. Note Peptide I and II inhibited the phosphorylation of pRb and induced Gi/S cell cycle arrest. This figure was modified from Ref [120] therefore, see Ref. [120] for details...

Annurca apple polyphenol extract (APE) HaCaT cells keratinocytes Antiproliferative action [104]... 

Wang Y, Bell JC, Keeney DS, Strobel HW (2010) Gene regulation of CYP4F11 in human keratino-cyte HaCaT cells. Drug Metab Dispos 38 100-107... 

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