Dermal fibroblasts

Gallucci RM, Lee EG, Tomasek JJ. IL-6 modulates alpha-smooth muscle actin expression in dermal fibroblasts from IL-6-deficient mice. J Invest Dermatol 2006 126(3) 561-568. 

It has been demonstrated in other cell types that lutein can inhibit expression of MMPs and/ or activity (Philips et al., 2007). For example, in dermal fibroblasts lutein inhibits expression of MMP-1 and decreases levels of MMP-2 protein (Philips et al., 2007). In melanoma cells, lutein inhibits MMP-1 expression while stimulating TIMP-2 (Philips et al., 2007). Moreover it has been shown that lutein inhibits elastin expression in fibroblasts subjected to oxidative stress by exposure to ultraviolet light (Philips et al., 2007). These results clearly indicate that lutein can play an important role in remodeling of the extracellular matrix. 

Philips, N, Keller, T, Hendrix, C, Hamilton, S, Arena, R, Tuason, M, and Gonzalez, S, 2007. Regulation of the extracellular matrix remodeling by lutein in dermal fibroblasts, melanoma cells, and ultraviolet radiation exposed fibroblasts. Arch Dermatol Res 299, 373-379. 

Chiang, H-S, W-B Wu, J-Y Fang et al. 2007. Lycopene inhibits PDGF-BB-induced signaling and migration of human dermal fibroblasts through interaction with PDGF-BB. Life Sci 81 1509-1517. 

Wu, W.B., Chiang, H.S., Fang, J.Y., and Hung, C.F. 2007. Inhibitory effect of lycopene on PDGF-BB-induced signalling and migration in human dermal fibroblasts A possible target for cancer. Biochem Soc Trans 35 1377-1378. 

The grating-coupled nanoporous-silica-supported reverse waveguide chip was also applied for monitoring the attachment and spreading of Human Dermal Fibroblast cells to the surface16. As in the bacteria experiments, the waveguide surface was coated with a thin layer of poly-L-lysine layer to improve cell attachment and spreading. 

Blander G, Guarente L (2004) The Sir2 family of protein deacetylases. Annu Rev of Biochem 73 417 35 Brinkmann H, Dahler AL, Popa C, Serewko MM, Parsons PG, Gabrielli BG, Burgess AJ, Saunders NA (2001) Histone hyperacetylation induced by histone deacetylase inhibitors is not sufficient to cause growth inhibition in human dermal fibroblasts. J Biol Chem 276 22491-22499... 

Type XII and XIV collagens may play a role in the organization of the collagen fihrils. When a small aliquot of type XII and XIV collagens is added to the collagen gel with dermal fibroblasts, the gel contracts in a dose-dependent manner. " The N-terminal NC3 domain is responsible for this activity. 

E) Retroviral vector expressing B-domain deleted factor VIII transfected into dermal fibroblasts that are then reimplanted... 

Wlaschek, M., Wenk, J., Brenneisen, R, Briviba, K., Schwarz, A., Sies, H., and Scharfetter-Kochanek, K. 1997. Singlet oxygen is an early intermediate in cytokine dependent UVA inducetion of interstitial collagenase in human dermal fibroblasts in vitro. FEES Lett. 413 239M2. 

The investigators evaluated the safety of their nonviral somatic-cell gene therapy system, which they call transkaryotic implantation, in six patients with severe hemophilia. The procedure involved isolation of dermal fibroblasts from the patients upper arms. The fibroblasts were then transfected with a factor VIII genebearing plasmid. Cells that expressed factor VIII were cloned, propagated, and implanted into the patients abdomens. This technique can be considered as a less invasive form of ex vivo gene therapy. 

PTPS activity may be determined from red blood cells (RBC), cultivated primary dermal fibroblasts, or from lysates of tissue samples (e.g., liver or brain). 

Reference and pathological values reference values for SR in (nonstimulated) dermal fibroblasts are for control cells 138 pU/mg (50th percentile 99 pU/mg for the 5th percentile, and 185 pU/mg for the 95th percentile), and for autosomal recessive SR deficiency <10 pU/mg. The reference values for amniocytes are 143 pU/mg (50th percentile 89 pU/mg for the 5th percentile, and 313 pU/mg for the 95th percentile). Mouse liver has an activity of around 150 pU/mg. For references see [26-29] and unpublished results from our laboratory. 

TCL, Cell transformation, immortalized hamster dermal fibroblasts (4DH2)... 

Shiner, A.C., Newbold, R.F. Cooper, C.S. (1988) Morphological transformation of immortalized hamster dermal fibroblasts following treatment with simple alkylating carcinogens. Carcinogenesis, 9. 1701-1709... 

Bae JY, Choi JS, Choi YJ, Shin SY, Kang SW, Han SJ, Kang YH. 2008. (-)Epigallo-catechin gallate hampers collagen destruction and collagenase activation in ultra-violet-B-irradiated human dermal fibroblasts Involvement of mitogen-activated protein kinase. Food Chem Toxicol 46 1298-1307. 

SERCA2a is expressed predominantly in the heart, in slow twitch skeletal muscle and in the brain, where it represents the main SERCA isoform. In contrast, SERCA2b is a house-keeping isoform, ubiquitously expressed in smooth muscle and non-muscle tissue (Wuytack et al., 2002). Although both isoforms are detectable in keratinocytes and dermal fibroblasts in culture, SERCA2b is the major isoform expressed in the epidermis from adult skin sections. SERCA2c is expressed in epithelial, mesenchymal and hematopoietic cell lines, and in monocytes (Table 1). 

Fig. 19 Phase-contrast microscope image of human dermal fibroblast cells attached to and spread on the surface of the waveguide chip...

Jinnin M, Dm H, Yamane K, Tamaki K. Interleukin-13 stimulates the transcription of the human oc2(I) collagen gene in human dermal fibroblasts. Journal of Biological Chemistry 2004, 279, 41783 11791. 

Hutcheson KA, Atkins BZ, Hueman MT Hopkins MB, Glower DD, Taylor DA, Comparing the benefits of cellular cardio-myoplasty with skeletal myoblasts or dermal fibroblasts on myocardial performance, Cell Transpl 2000 9 359-368. 

RGDS-PEG PEG-DA Human dermal fibroblasts Two-photon laser scanning photolithography to crosslink peptide-PEG inside a precrosslinked hydrogel to create 3D cell adhesion patterns 2008 [175]... 

The dermal fibroblast provides the synthetic machinery for dermal HA, and should be the target for pharmacological attempts to enhance skin hydration. The fibroblasts of the body, the most banal of cells from a histologic perspective, is probably the most diverse of all vertebrate cells with the broadest repertoire of biochemical reactions and potential pathways for differentiation. Much of this diversity is site specific. What makes the papillary dermal fibroblast different from other fibroblasts is not known. However these cells have an HA synthetic capacity similar to that of the fibroblasts that line joint synovium, responsible for the HA-rich synovial fluid (R. Stern, unpubl. exp.). 

Stimulation of HA synthesis also occurs following phorbol ester (PMA) and PDGF treatment, although a direct effect on HAS has not been demonstrated. Glucocorticoids induce a nearly total inhibiton of HAS mRNA in dermal fibroblasts and osteoblasts.183 Extracts of dermal fibroblasts indicate that HAS-2 is the predominant HA synthase therein. This may be the molecular basis of the decreased HA in glucocortcoid-treated skin. However, an additional effect on rates of HA degradation has not been examined. 

Fruit compresses have been applied to the face as beauty aids for millennia. The alpha-hydroxy acids contained in fruit extracts, tartaric acid in grapes, citric acid in citrus fruits, malic acid in apples, mandelic acid in almond blossoms and apricots are thought to be active principles for skin rejuvenation. Such alpha-hydroxy acids do stimulate HA production in cultured dermal fibroblasts (unpubl. exp.). The results of such alkaline preparations may depend more on their peeling effects rather than on the ability of alpha-hydroxy acids to stimulate HA deposition. 

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