Kidney cells

In the procedure for the surface test (313), the vims is grown in a monolayer of baby hamster kidney cells and incubated in Eagles medium supplemented with tryptose phosphate broth and calf semm. After separation of the vims from the cells by sonification and centrifugation, amounts of the suspension containing 3 x 10 plaque-forrning units are dried on coversHps. The inoculated coversHps are placed in 5 ml of the disinfectant for 1, 5, or 10 min, then rinsed, sonicated, and assayed. 

Another application shows the preparative purification and polishing of a therapeutic fusion protein with a humanized recombinant IgG protein. The fusion protein was expressed by the fermentation of baby hamster kidney cells. The filtered culture supernatant (155 liters) contained 2.2 g of IgG and 75.5 g of total protein. After the immunoglobulins were isolated by expanded bed adsorption and rebuffering, the IgG fraction was bound to Fractogel EMD SOj (M). This column achieved baseline separation of complete antibodies (fusion protein) from small amounts of antibodies lacking the fusion part. The resulting highly purified IgG fraction (110 ml) was diluted to 150 ml and... 

Kidney cells grown in culture with liver cells seek out and make contact with other kidney cells and avoid contact with liver cells. Cells grown in culture grow freely until they make contact with one another, at which point growth stops, a phenomenon well known as contact inhibition. One important characteristic of cancerous cells is the loss of contact inhibition. 

FIGURE 2.11 Receptor-occupancy curves for activation of human calcitonin type 2 receptors by the agonist human calcitonin. Ordinates (response as a fraction of the maximal response to human calcitonin). Abscissae (fractional receptor occupancy by human calcitonin). Curves shown for receptors transfected into three cell types human embryonic kidney cells (HEK), Chinese hamster ovary cells (CHO), and Xenopus laevis melanophores. It can be seen that the different cell types lead to differing amplification factors for the conversion from agonist receptor occupancy to tissue response. 

Human embryonic kidney cells, 21 Human genome, 2 Hydrogen bonding, 10 Hypothesis testing definition of, 239 description of, 227, 233 dose-response curves for, 239-243 F-test, 242t... 

A good example of the problems encountered with the use of serum with primary cultures is illustrated by the case with cultured kidney cells. The kidney epithelial cell line MDCK grows in serum-free medium supplemented with five supplements ... 

Figure 10. Primary cultures of mouse kidney cells. Primary cultures of kidney epithelial cells derived from 10-day-old mice were grown either in hormonally defined medium with five supplements (5 pg/ml insulin, 5 pg/ml transferrin, 25 ng/ml PCE, 5 X10" M hydrocortisone, and 5 x 10" M Tj), or in medium supplemented with 10% fetal calf serum. After 10 days, primary cultures still were epithelial in morphology serum free (a) but were overgrown with fibroblasts with serum (b). (Taub et al., 1979 with permission.)...

The normal rat kidney (NRK) cell line is an untransformed cell line established from rat kidney cells. However, when NRK cells are treated with both TGF alpha and TGF beta, the cells assume a transformed phenotype. In addition to exhibiting an altered morphology in monolayer culture, NRK cells possess the capacity for anchorage-independent growth while being treated with both TGF alpha and TGF... 

III in vitro and in cultured Xenopus kidney cells [60]. Further studies used polyamides in combination with recombinant derivatives of TFIIIA subunits to elucidate essential minor groove contacts for the binding of this TF (Tab. 3.1) [62]. 

Irvine JD, Takahashi L, Lockhart K, Cheong J, Tolan JW, Selick HE, et al. MDCK (Madin-Darby canine kidney) cells a tool for membrane permeability screening. J Pharm Sci 1999 88 28-33. 

Myers SJ, Wong LM, Charo IF (1995) Signal transduction and ligand specificity of the human monocyte chemoattractant protein-1 receptor in transfected embryonic kidney cells. J Biol Chem 270 5786-5792... 

Lash LH, Xu Y, Elfarra AA, et al. 1995. Glutathione-dependent metabolism of trichloroethylene in isolated liver and kidney cells of rats and its role in mitochondrial and cellular toxicity. Drug Metabolism and Disposition 23 846-853. 

The procedure for purification of Na,K-ATPase in membrane-bound form from the outer renal medulla of mammalian kidney offers the opportunity of exploring the structure of the Na,K-pump proteins in their native membrane environment. The protein remains embedded in the membrane bilayer throughout the purification procedure thus maintaining the asymmetric orientation of the protein in the baso-lateral membrane of the kidney cell in the purified preparation. This preparation has been particularly useful in studies of ultrastructure, protein conformation and for... 

Irvine, J. D., Takahashi, L., Lockhart, K., Cheong, J., Tolan, J. W., Selick, H. E., Grove, J. R. MDGK (Madin-Darby canine kidney) cells a tool for... 

Con A Concanavalin A COPD Chronic obstructive pulmonary disease COS Fibroblast-like kidney cell line established from simian cells CoVF Cobra venom CP Creatine phosphate Cp Caeruloplasmin c.p.m. Counts per minute CPJ Cartilage/pannus junction Cr The chemical symbol fir chromium CR Complement receptor CRl, CR2 CR4 Complement receptor types 1, 2 and 4 CR3-a Complement receptor type 3-[Pg.281]

Terashima, I. Suzuki, N. Shibutani, S. Mutagenic properties of estrogen quinone-derived DNA adducts in simian kidney cells. Biochemistry 2001, 40, 166-172. 

Labejof L, Berry J-P, Duchambon P, et al. 1998. Apoptosis of rat kidney cells after 241-Americium administration. Anticancer Res 18 2409-2414. 

Acetylcholinesterase [79] Kidney-cell plasminogen Bovine serum albumin [93]... 

Haase, R. et al., The phospholipid analogue hexadecylphosphatidylcholine inhibits phosphatidyl biosynthesis in Madin-Darby Canine Kidney Cells, FEBS Lett. 288, 129-132 (1991). 

J Ubl, H Murer, HA Kolb. Ion channels activated by osmotic and mechanical stress in membranes of opossum kidney cells. J Membrane Biol 104 223-232, 1988. 

MDCK (Madin-Darby canine kidney) cells are derived from distal tubules, whereas LLC-PKi are from proximal tubes. b BMEC (brain microvessel endothelial cells) are isolated from capillaries. BPAEC (bovine pulmonary artery endothelial cells), BAEC (bovine aortic endothelial cells), and HUVEC (human umbilical vein endothelial cells) are large vessel endothelia. 

JM Anderson, BR Stevenson, LA Jesaitis, DA Goodenough, MS Mooseker. (1989). Characterization of ZO-1, a protein component of the tight junction from mouse-liver and Madin-Darby canine kidney cells. J Cell Biol 106 1141-1149. 

In some human studies where clinical chemistry measurements but no renal biopsies were performed, the only parameter of renal function shown to be affected was an increase in the levels of NAG in the urine. NAG is a lysosomal enzyme present in renal tubular cells that has been shown to be a sensitive indicator of early subclinical renal tubular disease. The mechanism by which lead affects the release of NAG from renal tubular cells is not known, but it is suggested that lead could attach to kidney cell membranes and alter membrane permeability (Chia et al. 1994). 

Wang, F., Nelson, M., Kuryatov, A. et al. Chronic nicotine treatment upregulates human a3 32 but not a3P4 acetylcholine receptors stably transfected in human embryonic kidney cells. J. Biol. Chem. 1998 28721, 1998. 

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