Xenopus laevis melanophores

FIGURE 2.11 Receptor-occupancy curves for activation of human calcitonin type 2 receptors by the agonist human calcitonin. Ordinates (response as a fraction of the maximal response to human calcitonin). Abscissae (fractional receptor occupancy by human calcitonin). Curves shown for receptors transfected into three cell types human embryonic kidney cells (HEK), Chinese hamster ovary cells (CHO), and Xenopus laevis melanophores. It can be seen that the different cell types lead to differing amplification factors for the conversion from agonist receptor occupancy to tissue response. 

FIGURE 3.12 Dependence of constitutive receptor activity as ordinates (expressed as a percent of the maximal response to a full agonist for each receptor) versus magnitude of receptor expression (expressed as the amount of human cDNA used for transient transfection, logarithmic scale) in Xenopus laevis melanophores. Data shown for human chemokine CCR5 receptors (open circles), chemokine CXCR receptors (filled triangles), neuropeptide Y type 1 receptors (filled diamonds), neuropeptide Y type 2 receptors (open squares), and neuropeptide Y type 4 receptors (open inverted triangles). Data recalculated and redrawn from [27],... 

FIGURE 5.10 Effects of co-expressed G-protein (G ) on neuropeptide NPY4 receptor responses (NPY-4). (a) Dose-response curves for NPY-4. Ordinates Xenopus laevis melanophore responses (increases light transmission). Ordinates logarithms of molar concentrations of neuropeptide Y peptide agonist PYY. Curves obtained after no co-transfection (labeled 0 jig) and co-transfection with cDNA for Gai6. Numbers next to the curves indicate jig of cDNA of Ga]g used for co-transfection, (b) Maximal response to neuropeptide Y (filled circles) and constitutive activity (open circles) as a function of pg cDNA of co-transfected G g. 

Xenopus laevis melanophores, 82 yeast cells used in, 81-82 Furchgott method, 92, 95, 97-98, 261 Furosemide, 150, 151f... 

Minamoto T, Shimizu I 2002 A novel isoform of vertebrate ancient opsin in a smelt fish, Plemglossus altivelis. Biochem Biophys Res Commun 290 280—286 Miyashita Y, Moriya T, Yamada K et al 2001 The photoreceptor molecules in Xenopus tadpole tail fin, in which melanophores exist. Zoolog Sci 18 671—674 Moriya T, Miyashita Y, Arai J, Kusunoki S, Abe M, Asami K 1996 Light-sensitive response in melanophores of Xenopus laevis I. Spectral characteristics of melanophore response in isolated tail fin of Xenopus tadpole. J Exp Zool 276 11-18 Moutsaki P, Bellingham J, Soni BG, David-Gray ZK, Foster RG 2000 Sequence, genomic structure, and tissue expression of carp (Cyprinus carpio L.) vertebrate ancient (VA) opsin. FEES Lett 473 316-322... 

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