Human Embryonic Kidney 293 cells

FIGURE 2.11 Receptor-occupancy curves for activation of human calcitonin type 2 receptors by the agonist human calcitonin. Ordinates (response as a fraction of the maximal response to human calcitonin). Abscissae (fractional receptor occupancy by human calcitonin). Curves shown for receptors transfected into three cell types human embryonic kidney cells (HEK), Chinese hamster ovary cells (CHO), and Xenopus laevis melanophores. It can be seen that the different cell types lead to differing amplification factors for the conversion from agonist receptor occupancy to tissue response. 

Human embryonic kidney cells, 21 Human genome, 2 Hydrogen bonding, 10 Hypothesis testing definition of, 239 description of, 227, 233 dose-response curves for, 239-243 F-test, 242t... 

Wang, F., Nelson, M., Kuryatov, A. et al. Chronic nicotine treatment upregulates human a3 32 but not a3P4 acetylcholine receptors stably transfected in human embryonic kidney cells. J. Biol. Chem. 1998 28721, 1998. 

In addition to direct enhancement of channel activity, PTKs can indirectly increase GABA-evoked inhibitory current by recruiting intracellular GABAaR to the surface of postsynaptic membrane. Insulin has been shown to increase surface expression GABAaR in transfected human embryonic kidney cells. In central neurons insulin rapidly increases the expression of functional postsynaptic GABAaR in a tyrosine kinase-dependent manner, resulting in an increase in the amplitude of the miniature inhibitory postsynaptic currents. 

PR. Wielinga, G. Reid, E.E. Challa, I. van der Heijden, L. van Deemter, M. de Haas, C. Mol, A.J. Kuil, E. Groeneveld, J.D. Schuetz, C. Brouwer, R.A. De Abreu, J. Wijnholds, J.H. Beijnen, and P. Borst. Thiopurine metabolism and identification of the thiopurine metabolites transported by MRP4 and MRP5 overexpressed in human embryonic kidney cells. Mol Pharmacol. 62 1321-1331 (2002). 

Figure 22. Human embryonic kidney cells (A), rat vascular smooth muscle cells (B, C) and human osteoblast-like MG 63 cells (D) in cultures on micropattemed surfaces. A, B PTFE irradiated with UV light produced by a Xe2 -excimer lamp for 30 min in an ammonia atmosphere through a mask with holes 100 pm in diameter and center-to-center distance 300 pm C PE irradiated with Ar ions (energy 150 keV, ion dose lO ions/cm ) through a mask with holes 100 pm in diameter and center-to-center distance 200 pm fullerenes Qo deposited through a mask with rectangular holes with an average size of 128 3 pm per 98 8 pm on glass coverslips. Day 7 after seeding. A native cells in an inverted phase-contrast microscope B, C cells stained with hematoxylin and eosin, Olympus microscope IX 50 D cells stained with fluorescence-based LIVE/DEAD viability/cytotoxicity kit (Invitrogen), Olympus microscope IX 50. Bars 300 pm (A), 200 pm (B, D), Imm (C) [10,11].

Cui et al. [19] SWCNT Human embryonic kidney cells (HEK 293) in solution with 0.78-200 mg/ mL of SW CNT Induction of apoptosis and reduction of adhesion ability (and corresponding genes), reduction of cellular proliferation... 

Interactions with drugs have also been demonstrated for flavonoids from citrus. Naringin and naringenin were shown to interact with simvastatin and saquinavir in in vitro experiments (41,42) and caused alterations in the pharmacokinetics of quinine in rats (44). Moreover, naringenin and naringin were found to inhibit the OATP-B-mediated uptake of estrone-3-sulfate into human embryonic kidney cells (23). 

Regarding interactions between citrus and OATP, data from in vitro, animal and also human clinical studies are available. These studies demonstrated the inhibition of OATP-A in HeLa cells and of OATP-B mediated uptake of estrone-3-sulfate in human embryonic kidney cells and also in the oral intake of fexofenadine in rats by fruit juices, including GFJ and orange juice. Human clinical trials suggest a potential role of OATP in grapefruit-drug interactions using fexofenadine as substrate (22,23,67,78,79). 

Melliti K, Grabner M, Seabrook GR (2003) The familial hemiplegic migraine mutation R192Q reduces G-protein-mediated inhibition of P/Q-type (Ca(V)2.1) calcium channels expressed in human embryonic kidney cells. J Physiol 546 337-347. 

Starting in 1993, reports have appeared that in rat striatum and nucleus accum-bens D2-like autoreceptors, when activated, enhanced removal of dopamine from the extracellular space by means of the dopamine transporter (DAT) as studied by voltammetry (Table2). D2 receptor activation also increased [3H]-dopamine uptake in Xenopus oocytes transfected with both the human D2 receptor and the human DAT (Mayfield and Zahniser 2001), as well as the uptake of a fluorescent DAT substrate in human embryonic kidney cells equally transfected with both the human D2 receptor and the human DAT (Bolan et al. 2007). In accord with this autoreceptor-transporter connection, clearence of dopamine from the striatal extracellular space in vivo was reduced in D2 receptor knockout mice (Dickinson et al. 1999). Unfortunately, however, the data in the literature are by no means consistent. No effect of D2 receptor activation on the DAT was observed in another rat striatum study (Prasad and Amara 2001), in synaptosomes from rat and human neocortex (Feuer-stein, unpublished observations), and in PC 12 cells that possess D2 receptors and the DAT (Pothos et al. 1998). In a second study with D2 receptor knockout mice, uptake of dopamine in the striatum was increased rather than decreased (Schmitz et al. 2002). The reason for these discrepancies is not clear. It has been suggested that the clearance measurements by means of voltammetry were not appropriate for assessment of the function of the DAT (Prasad and Amara 2001). 

HEC hydroxyethylcellulose HEK human embryonic kidney cells HEMA hydroxyethylmethacrylate... 

Indirect protein patterning Serum proteins from cell culture medium PDMS, methylated glass, bacterial grade PS Human epithelial cells, human keratinocytes, human neuroblastoma cells, human breast cancer cells, human fibroblasts, human embryonic kidney cells, murine macrophages Stencil guided plasma induced oxidation of hydrophobic surfaces 2009 [93]... 

Rat skeletal myoblasts, human embryonic kidney cells, colon carcinoma cells pCP of etching solution or UV ablation of PVA 2006 [98]... 

Stride BD, Grant CE, Loe DW, et al. Pharmacological characterization of the murine and human orthologs of multidrug-resistance protein in transfected human embryonic kidney cells. Mol Pharmacol 1997 52 344-353. 

HEK-293 hGH High-Five hsp70 HSV IPTG IRES kb Lac LCR LoxP LUC MCS human embryonic kidney cells human growth hormone TM BTI-TN-5B1-4 (cell line derived from the insect Trichoplusia ni) heat shock protein 70 herpes simplex virus isopropyl 1 -thio-fi-D-galactopyranoside internal ribosomal entry site kilobases lactose operon/repressor locus control region locus of crossover of PI luciferase isolated from firefly multiple cloning site... 

Marchand P, Resch K, Radeke HH. Selective inhibition of monocyte chemoattractant protein-1 gene expression in human embryonal kidney cells by specific triple heUx-forming oUgonucleotides. J. Immunol. 2000 164 2070-2076. 

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