Madine-Darby canine kidney cells

Irvine JD, Takahashi L, Lockhart K, Cheong J, Tolan JW, Selick HE, et al. MDCK (Madin-Darby canine kidney) cells a tool for membrane permeability screening. J Pharm Sci 1999 88 28-33. 

Irvine, J. D., Takahashi, L., Lockhart, K., Cheong, J., Tolan, J. W., Selick, H. E., Grove, J. R. MDGK (Madin-Darby canine kidney) cells a tool for... 

Haase, R. et al., The phospholipid analogue hexadecylphosphatidylcholine inhibits phosphatidyl biosynthesis in Madin-Darby Canine Kidney Cells, FEBS Lett. 288, 129-132 (1991). 

MDCK (Madin-Darby canine kidney) cells are derived from distal tubules, whereas LLC-PKi are from proximal tubes. b BMEC (brain microvessel endothelial cells) are isolated from capillaries. BPAEC (bovine pulmonary artery endothelial cells), BAEC (bovine aortic endothelial cells), and HUVEC (human umbilical vein endothelial cells) are large vessel endothelia. 

JM Anderson, BR Stevenson, LA Jesaitis, DA Goodenough, MS Mooseker. (1989). Characterization of ZO-1, a protein component of the tight junction from mouse-liver and Madin-Darby canine kidney cells. J Cell Biol 106 1141-1149. 

Madin-Darby canine kidney cell line... 

S. J., Goldenberg, S., Wiliams, C., Pasatan, I., Gottesman, M. M., Handler, J., Transepithelial transport of drugs by the multidrug transporter in cultured Madin-Darby canine kidney cell epifhelia, J. Biol. Chem. 1989, 264, 14880-14884. 

L. M., Feng, B., Giacomini, K. M., Functional characteristics and steroid hormone-mediated regulation of an organic cation transporter in Madin-Darby Canine Kidney cells, J. Pharmacol. Exp. Ther. 2001, 299, 392— 398. 

LLC-PKi Porcine kidney cell line used as absorption model MDCK Madin-Darby canine kidney cell line used as absorption model... 

MDCK Madin-Darby canine kidney cells... 

In vitro assays are increasingly being used. Some of the reasons are cost, availability of more rapid results, and avoidance of negative publicity. Assays such as cytochrome P-450 enzymes, the Ames test, and the mouse lymphoma tk test are in vitro methods. For absorption studies, Caco-2 (Exhibit 5.9) and Madin-Darby canine kidney cell assays are now routinely used. Hepatocyte cell lines with metabolism capacity are being developed to test drug metabolism and toxicity. All these examples show that, where possible, pharmaceutical firms are gradually dispensing with animal studies. 

Yamada A, Irie K, Hirota T, Ooshio T, Fukuhara A, Takai Y. 2005. Involvement of the annexin II-S100A10 complex in the formation of E-cadherin-based adherens junctions in Madin-Darby canine kidney cells. J Biol Chem 280(7) 6016-6027. 

Tl. Takeda, T., Go, W. Y., Orlando, R. A., and Farquhar, M. G., Expression of podocalyxin inhibits cell-cell adhesion and modifies junctional properties in Madin—Darby canine kidney cells. 

Hunziker W, Mellman I. Expression of macrophage-lymphocyte Fc receptors in Madin-Darby canine kidney cells polarity and transcytosis differ for isoforms with or without coated pit localization domains. / Cell Biol 1989 109(6 Pt 2) 3291-302. 

Martens, A., Bode, J. G., Heinrich, P. G., and Graeve, L. (2000). The cytoplasmic domain of the interleukin-6 receptor gp80 mediates its basolateral sorting in polarized madin-darby canine kidney cells./. Cell Sci. 113, 3593-3602. 

Sheikh-Hamad, D. el al., 1998, p38 Kinase activity is essential for osmotic induction of mRNAs for HSP70 and transporter for organic solute betaine in Madin-Darby canine kidney cells. J. Biol. Chem. 273(3) 1832-1837. 

Uchida, S., Yamauchi, A., Preston, A.S., Kwon, H.M., and Handler, J.S., 1993, Medium tonicity regulates expression of the NaO and Cl( - )-dependent betaine transporter in Madin-Darby canine kidney cells by increasing transcription of the transporter gene. J. Clin. Invest. 91(4) 1604-1607. 

Tajarobi, F. El-Sayed, M. Rege, B.D. PoUi, J.E. Ghandehari, H. Transport of polyamidoamine dendrimers across madin-darby canine kidney cells. Int. J. Pharm. 2001, 215, 263-267. 

Zlatkine P, Moll G, Blais A, Lolseau A, and Le Grimellec C. Transport of choline by Madin-Darby canine kidney cells. Biochim Biophys Acta 1153 237-242,1993. 

Singh ABand Flarris RC. Epidermal Growth Factor Receptor Activation Differentially Regulates Claudin Expression and Enhances Transepithelial Resistance in Madin-Darby Canine Kidney Cells. J Biol Chem 279 3543-3552,2004. 

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