Kidney cells culture

Taub, M. (1984). Growth of primary and established kidney cell cultures in serum-free media. In Methods for Serum-Free Culture of Epithelial and Fibroblastic Cells (Barnes, D.W., Sirbasku, D.A.A. and Sato, G.H., Eds.). Alan R. Liss, New York, pp. 3-24. 

Finally, it is worth repeating the statement of the US Centers for Disease Control and Prevention, issued in 1992 but still valid (17) The suggestion that HIV, the AIDS virus, originated as a result of inadvertent inoculation of an HIV-like virus present in monkey kidney cell cultures used to prepare polio vaccine is one of a number of unsubstantiated hypotheses. The weight of scientific evidence does not support this idea and there is no more reason to believe this hypothesis than many other which have been considered and rejected on scientific grounds. ... 

Polatnick J Bacharach HL (1964) Production and purification of milligram amounts of foot-and-mouth disease virus from baby hamster kidney cell culture. Applied Microbiology 12 368-373. 

A more general example from virus vaccine production is the rigorous examination of tissue cultures to exclude contamination with infectious agents from the source animal or, in the cases of human diploid cells or cells from continuous cell lines, to detect cells with abnormal characteristics. Monkey kidney cell cultures are tested for simian herpes B virus, simian virus 40, mycoplasma and tubercle bacilli. Cultures of human diploid cells and continuous line cells are subjected to detailed kary-ological examination (examination of chromosomes by microscopy) to ensure that the cells have not undergone any changes likely to impair the quality of a vaccine or lead to undesirable side-effects. 

POLIOVIRUS VACCINE, INACTIVATED (IPV) (IPOL injection suspension of 3 types of poliovirus (types 1,2, and 3) grown in monkey kidney cell cultures)... 

Urokinase Human kidney cell culture < 20 min Active plasminogen activator... 

ANF has potent effects on cyclic GMP (cGMP) levels. In vitro, addition of atrial extracts to minced kidney tissue or to primary kidney cell cultures results in increased levels of cGMP.H Injection into anesthetized rats resulted in a 4-fold increase in plasma cGMP levels and a 28-fold increase in urinary excretion.Sodium nitroprusside also causes increased cGMP levels in treated tissues, but probably acts directly through stimulation of guanylate cyclase. Further studies must be done to clarify the role of cGMP in the vasorelaxant and the natriuretic actions of ANF. 

Gliotoxin (308) is a fungal metabolite, isolated from Aspergillus terreus and found to have antibacterial, antitumor and antifungal effects [18]. Gliotoxin acetate inhibited the CPE of poliovirus in monkey kidney cell cultures due to the early stage inhibition of RNA viral replication [18]. Gliotoxin also inhibits influenza virus-induced RNA polymerase. 

Human Embryonic Kidney cell. Cultured cells, frequently used for biological experiments on the cell level. 

Faucet-Marquis, V., Pont, F., Stormer, F.C., Rizk, T., Castegnaro, M. Rohl-Leszkowicz, A. (2006) Evidence of a new dechlorinated ochratoxin A derivative formed in opossum kidney cell cultures after pretreatment by modulators of glutathione pathways correlation with DNA adduct formation. Mol. Nutr. Food Res. 50, 530-542. 

In an assessment of the vitro susceptibility of HSV to antiviral drugs, utilizing a plaque reduction assaya rabbit kidney cell culture system produced lower MIC s than did a hamster kidney cell system. Of the two types of HSV tested (types I and II, a total of 21 strains), both exhibited an vitro spectrum for susceptibility to IHDR, which may account for some of the differences previously reported. HSV-I was most consistently inhibited by lUDR and Ara-C, while Ara-C was most effective against HSV-II. Adenine arablnoside (Ara-A) and hypoxanthine-arabinoside were less effective against both HSV types. 

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