Brain microvessel endothelial cells

Pal, D. Audus, K. L. Siahaan, T. J., Modulation of cellular adhesion in bovine brain microvessel endothelial cells by a decapeptide, Brain Res. 747, 103-113 (1997). 

MDCK (Madin-Darby canine kidney) cells are derived from distal tubules, whereas LLC-PKi are from proximal tubes. b BMEC (brain microvessel endothelial cells) are isolated from capillaries. BPAEC (bovine pulmonary artery endothelial cells), BAEC (bovine aortic endothelial cells), and HUVEC (human umbilical vein endothelial cells) are large vessel endothelia. 

Miller DW, KL Audus, RT Borchardt. (1992). Cultured bovine brain microvessel endothelial cells A model of the blood brain barrier. J Tiss Cult Meth 14 217-224. 

Regina A, Roman A, Piciotti M, El Hafny B, Center MS, Bergmann R et al. Mrpl multidrug resistance-associated protein and P-glycoprotein expression in rat brain microvessel endothelial cells. J Neurochem 1998 71(2)705-715. 

Huai-Yun H, Secrest DT, Mark KS, Carney D, Elmquist WF, Miller W. Expression of multi-drug resistance-associated protein (MRP) in brain microvessel endothelial cells. Biochem Biophys Res Commun 1998 243 816-820. 

PBEC Porcine brain microvessel endothelial cells... 

Preparation of Porcine Brain Microvessel Endothelial Cells... 

The rather time- and cost-expensive preparation of primary brain microvessel endothelial cells, as well as the limited number of experiments which can be performed with intact brain capillaries, has led to an attempt to predict the blood-brain barrier permeability of new chemical entities in silico. Artificial neural networks have been developed to predict the ratios of the steady-state concentrations of drugs in the brain to those of the blood from their structural parameters [117, 118]. A summary of the current efforts is given in Chap. 25. Quantitative structure-property relationship models based on in vivo blood-brain permeation data and systematic variable selection methods led to success rates of prediction of over 80% for barrier permeant and nonper-meant compounds, thus offering a tool for virtual screening of substances of interest [119]. 

Zhang, Y, Han, H, Elmquist, WF, MMiiler, DW (2000) Expression of various multidrug resistance-associated protein (MRP) homologues in brain microvessel endothelial cells. Brain Res 876 148-153. 

Franke H, Gallah HJ, Beuckmann CT (2000) Primary cultures of brain microvessel endothelial cells A valid and flexible method to study drug transport through the blood-brain barrier in vitro. Brain Res Prot 5 248-256... 

Shi F, Audus KL (1994) Biochemical characteristics of primary and passaged cultures of primate brain microvessel endothelial cells. Neurochem Res 19 427-433... 

Numerous modifications of in vitro culture systems have been developed for the estimation of BBB transfer [52]. Culture systems in use are either primary cultures of brain microvessel endothelial cells (BMEC) or immortalized endothelial cell hues. BMEC may be grown in co-culture with astrocytes or in astrocyte-conditioned medium. Astrocyte-derived factors increase the tightness of the barrier as measured by transendothelial electrical resistance (TEER) and by the permeability of hydrophUic markers such as sucrose. They also up-regulate the expression of BBB-enriched enzymes such as y-glutamyl transpeptidase (y-GTP) and alkaline phosphatase. A setup of the in vitro technique in a transwell system for transport studies is depicted in Figure 2.5. 

Miller, D. W, E. V. Batrakova, T. O. V foltner, V. Y. Alakhov, and A. V. Kabanov. 1997. Interactions of Pluronic block copolymers with brain microvessel endothelial cells evidence of two potential pathways for drug. Bioconj. Chem8 649-657. 

Miller, D., B. Keller, and R. Borchardt. 1994. Identification and distribution of insulin receptors on cultured bovine brain microvessel endothelial cells Possible function in insulin processing in the blood-brain barrier. J Cell Physiol 161 333. 

Thole, M., et al. 2002. Uptake of cationized albumin coupled liposomes by cultured porcine brain microvessel endothelial cells and intact brain capillaries. J Drug Target 10 337. 

Batrakova, E., et al. 2003. Optimal structure requirements for pluronic block copolymers in modifying P-glycoprotein drug efflux transporter activity in bovine brain microvessel endothelial cells. J Pharmacol Exp Ther 304 845. 

Audus KL, Ng L, Wang W, et al. Brain microvessel endothelial cell culture systems. Pharm Biotechnol 1996 8 239-258. 

Audus KL, Borchardt RT. Characteristics of the large neutral amino acid transport system of bovine brain microvessel endothelial cell monolayers. J Neurochem 1986 47(2) 484 188. 

Audus KL, Borchardt RT. Bovine brain microvessel endothelial cell monolayers as a model system for the blood-brain barrier. Ann N Y Acad Sci 1987 507 9-18. 

Borges N, Shi F, Azevedo I, et al. Changes in brain microvessel endothelial cell monolayer permeability induced by adrenergic drugs. Eur J Pharmacol 1994 269 (2) 243-248. 

Guillot FL, Audus KL. Angiotensin peptide regulation of fluid-phase endocytosis in brain microvessel endothelial cell monolayers. J Cereb Blood Flow Metab 1990 10 (6) 827-834. 

Raub TJ, Audus KL. Adsorptive endocytosis and membrane recycling by cultured primary bovine brain microvessel endothelial cell monolayers. J Cell Sci 1990 97 (pt 1) 127—138. 

Fontaine M, Elmquist WF, Miller DW. Use of rhodamine 123 to examine the functional activity of P-glycoprotein in primary cultured brain microvessel endothelial cell monolayers. Life Sci 1996 59(18) 1521—1531. 

Bornstein MB (1958) Reconstituted rattail collagen used as substrate for tissue cultures on coverslips in Maximow slides and roller tubes. Lab Invest 7 134-137 Bowman PD, Ennis SR, Rarey KE et al. (1983) Brain microvessel endothelial cells in tissue culture a model for study of blood-brain barrier permeability. Ann Neurol 14 396-402 Eisenblatter T, Galla HJ (2002) A new multidrug resistance protein at the blood-brain barrier. Biochem Biophys Res Com-mun 293 1273-1278... 

Audus, K.L., Guillot, F.L. and Braughler, J.M. (1991) Evidence for 21-aminosteroid association with the hydrophobic domains of brain microvessel endothelial cells, Free Radical Biol. Med. 11, 361-371. 

BBMEC Bovine brain microvessel endothelial cell lines... 

Fischer S, Wobben M, Marti HH, Renz D, Schaper W (2002) Hypoxia-induced hyperpermeability in brain microvessel endothelial cells involves VEGF-mediated changes in the expression of zonula occludens-1. Microvasc Res 63 70-80 Fishman RA (1975) Brain edema. N Engl J Med 293 706-711... 

---