Enzyme immunosorbent assay

In cases where quantitative analyses give concentration values much lower than the specified MRL for a certain analyte, positive quantitative errors do not play a role in the no answer. However, negative quantitative errors may be important if a screening test such as the four-plate test for antibiotics or an enzyme-immunosorbent assay for -agonists is used, some specific analytes for which the test has no or very low response may slip through the holes (43). 

Schmitt, A., V. Hingst, L. Erdinger, et al. 1992. Aspects in the development of an enzyme immunosorbent-assay for the detection of the herbicide metolachlor in water samples. Zentral. Hyg. Umweltmed. 193 272-286. 

Ksiazek TG, Rollin PE, Jahrling PB, Johnson E, Dalgard DW, Peters CJ. Enzyme immunosorbent assay for Ebola virus antigens in tissues of infected primates. J Clin Microbiol. 1992 30(4) 947—950. 

Allen DG, Greirson BN, Harris DJ. An enzyme immunosorbent assay (ELISA) for lupin alkaloids Comparison with gas chromatography. In von Baer D, editOT. International lupin Association sixth international lupin conference proceedings. November 25—30, 1990. Temuco-Pucon, Chile Asociacion Chilma del Lupino 1991. p. 406. 

An enzyme-linked immunosorbent assay (eflsa) has been developed for the detection of residues on hands. As Httle as 50 pg of TNT can be detected (126). Liquid chromatography/thermospray negative-ion tandem ms has been successfully used to detect picogram levels of explosives in post-blast debris (127). 

MacroHdes are obtained by controUed submerged aerobic fermentations of soil microorganisms. Although species of Streptomjces have dominated, species of Saccharopoljspora Micromonospora and Streptoverticillium are also weU represented. New techniques such as enzyme-linked immunosorbent assay (ELISA) based assays may prove beneficial for discovering new stmctures (464). 

In each of the assays of potency the amount of the immunoglobulin and the amount of a corresponding standard preparation that are required to neutralize the infectivity or other biological activity of a defined amount of virus or to neutralize a defined amount of a bacterial toxin are determined. The two determined amounts and the assigned unitage of the standard preparation are then used to calculate the potency of the immunoglobulin in International Units (lU). ELISA, enzyme-linked immunosorbent assay. 

HPLC, high-petfomtance liquid chromatogtaphy ELISA, enzyme-linked immunosorbent assay method RIA, radioimmunoassay method. 

Several heterogeneous electrochemical enzyme immunoassays have been demonstrated. These are based on the enzyme-linked immunosorbent assay (ELISA) technique... 

ELISA Enzyme-linked immunosorbent assay EMS Eosinophilia-myalgia syndrome ENS Enteric nervous system EO Eosinophil... 

Morishita, N., Kamiya, K., Matsumoto, T., Sakai, S., Teshima, R., Urisu, A., Moriyama, T., Ogawa, T., Akiyama, H., and Morimatsu, F. (2008). A reliable enzyme-linked immunosorbent assay for determination of soybean proteins in processed foods. /. Agric. Food Chem. 56, 6818-6824. 

H.A. Moye, Enzyme-linked immunosorbent assay (ELISA), in Pesticide Residues in Foods Methods, Techniques, and Regulations, W.G. Fong, H.A. Moye, J.N. Seiber, and J.P. Toth (eds), Wiley, New York, Chapt. 6 (1999). 

Watanabe et al. developed an enzyme-linked immunosorbent assay (ELISA) for the detection of inabenfide, a plant growth regulator, in rice. Specific monoclonal antibody (MAB) is used for this method. The effects of rice matrices on the sensitivity of ELISA can be reduced by adding 0.1% Tween 20. Good reproducibility and accuracy of the proposed ELISA were obtained for rice samples and the recovery was 92% at a fortification level of 5-500 xgkg . ... 

The need to understand the fate of pesticides in the environment has necessitated the development of analytical methods for the determination of residues in environmental media. Adoption of methods utilizing instrumentation such as gas chro-matography/mass spectrometry (GC/MS), liquid chromatography/mass spectrometry (LC/MS), liquid chromatography/tandem mass spectrometry (LC/MS/MS), or enzyme-linked immunosorbent assay (ELISA) has allowed the detection of minute amounts of pesticides and their degradation products in environmental samples. Sample preparation techniques such as solid-phase extraction (SPE), accelerated solvent extraction (ASE), or solid-phase microextraction (SPME) have also been important in the development of more reliable and sensitive analytical methods. 

TR. Dombrowski, E.M. Thurman, and G.B. Mohrman, A first application of enzyme-linked immunosorbent assay for screening cyclodiene insecticides in ground water, in Environmental Immunochemical Methods, ed. J.M. Van Emon, C.L. Ger-lach, and J.C. Johnson, American Chemical Society, Washington, DC, pp. 148-154 (1996). 

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