Enzyme-linked immunosorbent assay description

Linked with the previous basic description on antibodies, there is an other form of labelling, which employs an enzyme rather than a radioisotope. In this case the tag (the enzyme) is enormous with respect to the subject molecule. Since the concentrations are very low, recovery is undertaken using an antibody. The best known methodology of this type is the enzyme-linked immunosorbent assay... 

Enzyme immunoassays are broadly known as enzyme-linked immunosorbent assays (ELISA). See Ref. 13 for the original description. The different types are illustrated in Figure 24.7. These assays are conveniently performed by immobilizing... 

Pathol. 5, 38 (1983). Enzyme-linked immunosorbent assay of residues in foods W. H. Newsome, J. Agr. Food Chem. 33, 528 (1985). Comprehensive description P. A, Urech, F. J, Schwinn, Phytiatr -Phyiopharm, 27, 239-247 (1978). 

Depending on the label, lAs are classified in different groups. Radioisotopes are used in RIAs, enzymes in enzyme-linked immunosorbent assays (ELISAs) or EIAs, fluorophores in FIAs or PFIAs and chemiluminescent compounds in CLIAs. Additional types of lA exist, but are not very common in pesticide analysis. A more detailed description of these lAs can be found in Gosling. 

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