Enzyme Linked Immunosorbent Assay to Determine Adsorbed and Immobilized Proteins

Enzyme Linked Immunosorbent Assay (ELISA) To Determine Adsorbed and Immobilized Proteins 

To detect the adsorption of a protein at a biomaterial s surface appropriate ELISA tests, based on the specific reaction of antibodies with surface-bound antigens, are applied [86]. An indirect ELISA variant in which a primary antibody binds to the adsorbed protein (antigen) is shown in Fig. 8. In a second step a secondary antibody, which is conjugated with an enzyme like horse radish peroxi- 

The described assay (ELISA) is a semiquantitative technique and is based on a comparison of the spectroscopic absorption values with those obtained with reference materials. Tissue culture polystyrene (TOPS) is used as a reference surface with a high protein-binding capacity. The detection of the binding capacity of antibodies is useful to establish the amount of adsorbed and immobilized fi-bronectin. When a high amount of adsorbed or immobilized fibronectin exists 

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