Enzyme linked immunosorbent assay immunoreactivity

The successful of recovery of RNase A functional activity by a heat-induced AR method suggested the possibility of recovering RNase A immunoreactivity as well. The immunoreactivity of native RNase A and RNase A that was incubated at a concentration of 4 mg/mL in 10% neutral buffered formalin for 1 day and then freed of formaldehyde by dialysis against PBS was compared using capture enzyme-linked immunosorbent assay (ELISA). Selected fractions that... 

An example of an immunoassay is the enzyme-linked immunosorbent assay (ELISA), which uses 96-well plates with antibodies bound on the sides of the wells. The characteristic of interest is the binding constant for the immunoreaction, which is defined as the association constant between antibody and antigen. It is important to generate a good antibody with strong binding. 

Henares et al. [210] developed a multiple enzyme linked immunosorbent assay (ELISA) chip by using capillary-assembled microchip (CAs-CHIP), which involved simple embedding of a 2 to 3 mm length of square capillaries possessing valving and immunoreaction functions into the microchannels fabricated on a PDMS substrate. The authors immobilized different anti-IgGs... 

Recently we have developed polyclonal and monoclonal antibodies to Cu,Zn-SOD and Mn-SOD and have used these in an enzyme-linked immunosorbent assay (ELISA) for the SODs. We found that the level of immunoreactive SOD in tissues or fluids gives valuable information for biochemical and clinical studies (K5). The biochemical properties of EC SOD have been extensively studied by Marklund and his group (H13,K1,M5-M8), but its clinical significance requires further studies. 

The pancreatic lipase immunoreactivity (PLI) assay is a measure of lipase and its related precursors. While the function of pancreatic lipase is the same as any other lipase in the body, the molecular structure is unique. For this reason, a radioimmunoassay (RIA) and subsequently an enzyme-linked immunosorbent assay (ELISA) were developed and validated by the Westbrook, ME, US-based manufacturer, IDEXX Laboratories, Inc., for dogs and cats to measure canine and feline PLI (i.e., cPLI and fPLI, respectively). Relative to TLl, species-specific PLI assays have better sensitivity and specificity for detection of pancreatitis (Xenoulis, 2015). Based upon the available data, PLI appears to be the most sensitive laboratory test available for the diagnosis of pancreatitis. Although rat-reactive commercial PLI antibody is available, there is as yet a significant need to develop and validate an ELISA assay that can reliably detect the antigen in preclinical species. 

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