Enzyme-linked immunosorbent assay pantothenic acid

Radioimmunoassay has been used for assay of pantothenic acid in physiological samples (sensitivity 250 nmol 1 ), and an enzyme-linked immunosorbent assay (ELISA) has been used for this vitamin in food analysis. 

Radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA) procedures are no longer used for pantothenic acid determination because no commercially available antibody is available for these techniques. 

Morris, H.C., Finglas, P.M., Faulks, R.M., and Morgan, M.R.A., 1988. The development of an enzyme-linked immunosorbent assay (ELISA) for the analysis of pantothenic acid and analogues. Part I production of antibodies and establishment of ELISA system. Journal of Micronutrient Analysis. 4 33 5. 

Song W.O., Smith A., Wittwer C., Wyse B.W., and Hansen R.G., 1990. Determination of plasma pantothenic acid by indirect enzyme linked immunosorbent assay. Nutrition Research. 10 439-448. 

Pantothenic acid and pantetheine may be assayed using several enzymatic tests with pantothenase (40). Immunoassays and, especially, radioimmunoassays have been used in the analysis of pantothenic acid in tissue fluids, because of their sensitivity, specificity, and high rates of sample throughput. The radioimmunoassay (RIA) methods have about the same sensitivity as microbiological tests (approximately 0.05 pg/mL). As an alternative to the radioimmunoassay, the enzyme-linked immunosorbent assay (ELISA) is particularly suitable for the routine analysis of foods (41-45). 

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