Enzyme-linked immunosorbent assays performance/limitations

Analysis of taxanes is performed almost exclusively by high-performance liquid chromatography (HPLC). Immunological techniques [enzyme-linked immunosorbent assay (ELISA), radioimmunoassay (RIA)] have found minimal use in screening human plasma and plant extracts. Such biological methods suffer from cross reactivity of several taxanes with paclitaxel and their use is thus limited in semiquantitative aspects. 

High performance liquid chromatography (HPLC), the fluorometric-iodine method (FL-IM), the enzyme-linked immunosorbent assay (ELISA), the solid-phase radioimmunoassay (RIA), and the tandem mass spectrometer, all have great potential. Research is needed to determine the extent, limitations, and applications for each method. 

DTMA dodecyltrimethylammonium ELISA enzyme-linked immunosorbent assay GC-MS gas chromatography-mass spectrometry HPLC high-performance liquid chromatography LC-MS liquid chromatography-mass spectrometry LOQ limit of quantification 3-MPA 3-mercaptopropionic acid MS mass spectrometry... 

It is possible to perform an enzyme-linked immunosorbent assay (ELISA) using a streptavidin-coated 96-well plate the primary antibody, biotinylated-rabbit-anti-streptavidin, specifically recognizes the avidin linked to the helicate as the bioprobe. The detection limit is better than that with commercially available organic dyes such as antistreptavidin-FICT (FiB3). 

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