Enzyme-linked immunosorbent assay conjugate preparation

Enzyme-linked immunosorbent assay (ELISA) methods are widely used in medical and food analysis laboratories and it seems appropriate that such methods could be used for the detection of mycotoxins in foods. In the direct ELISA method the antibody is bound to a solid surface, such as a microtiter plate, and it is necessary to prepare a conjugate of the mycotoxin to be analyzed with the enzyme, such as phosphatase or peroxidase, to be used for color development. The sample or standard is mixed with the enzyme-linked mycotoxin and the two allowed to compete for the antibody bound to the surface. After washing away soluble material, the... 

For many applications, peptides should be conjugated to carriers. An important example is the conjugation of peptides to proteins, carbohydrates, or lipids for immunological studies. Further examples are the preparation of peptide affinity media and the conjugation of peptides to suitable coating compounds on siufaces for enzyme-linked immunosorbent assay and plasma resonance. 

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