Heat-Assisted Enzyme-Linked Immunosorbent Assay

MICROWAVE HEAT-ASSISTED ENZYME-LINKED IMMUNOSORBENT ASSAY 

Microwave heat-assisted enzyme-linked immunosorbent assay (ELISA) has been used for measuring anti-glomerular basement membrane (GBM) antibodies in kidney serum (Van Dorp et al., 1991) The presence of GBM antibodies is one of the characteristics of Goodpasture s syndrome. The application of microwave heating reduces the duration of incubation to assay circulating anti-GBM autoantibodies. 

Conventional incubation is carried out by placing 100 ml of the substrate (0.02% H202, 0.04 mg/ml OPD, 0.04M citric acid, and 0.05 M disodium hydrophosphate, pH 5.0) in each well for 20 min at room temperature. The reaction is stopped with 50p,l of 4 N-sulfuric acid per well. The optical density of the colored solutions is read at 492 nm with a multiphotospectrometer. 

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