General enzyme-linked immunosorbent assay procedure

For the determination of binding properties of biotinylated proteins and conjugates, use flexible 96-wells microplates (Nunc). The general design of the method is similar to classical enzyme-linked immunosorbent assay (ELISA) and RIA methods. Use the described procedure for immobilization of streptavidin, antigen, biotinylated or nonmodified antibody, or catalase. 

The procedures for production of specific antibodies and their application in a competitive inhibition ELISA (Enzyme-Linked Immunosorbent Assay) are discussed in detail in the preceding chapter (Vanderlaan et al., this volume). In addition, other comprehensive overviews of the immunoassay development process in the pesticide field are available in general, a... 

Heterogeneous enzyme immunoassay is by far the most common assay format used in ECI. Most of the heterogeneous assays are based on the enzyme-linked immunosorbent assay (ELISA) technique where Ah is immobilized on the walls of small (<500 gL) microtiter wells or cuvettes [105]. The general procedure of heterogeneous immunoassay is outlined... 

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