Enzyme-linked immunosorbent assay noncompetitive

E. Chueng, and A.A. Levin. 2002. Development of an ultrasensitive noncompetitive hybridization-ligation enzyme-linked immunosorbent assay for the determination of phosphorothioate oligodeoxynucleotide in plasma. Anal. Biochem. 304 19-25. 

Table 1 The plethora of formats of immunoassay. Ab = antibody, Ag = antigen. Noncompetitive and competitive heterogenous binding assays where the probe is labelled with an enzyme are known as enzyme-linked immunosorbent assay (ELISA)...

Figure 5 Schematic representation of the most widely used noncompetitive biologieal immunoassay, the enzyme-linked immunosorbent assay (ELISA), (a) Antibody, selective for analyte, is immobilized on microtiter plate well surface (b) sample added (c) analyte in sample binds to antibody while other compounds in matrix remain in solution (d) sample solution containing nonbound molecules discarded and wells washed (e) analyte remains bound to antibody (f) second antibody nzyme conjugate added (g) conjugate binds to bound analyte (h) solution containing nonbound conjugate discarded and wells washed (i) conjugate remains bound 0 enzyme substrate added (k) substrate S converted to colored or fluorescent product P at a rate which is proportional to the amount of bound enzyme and hence to the concentration of analyte in the sample.

Gelatin (2 mg/ml) and ribose (30 mM) were incubated in the presence of plant extracts (10 pg/ml) in 100 mM Sodium phosphate buffer at 37 C for 7 days, followed by the determination of CMA by a noncompetitive enzyme-linked immunosorbent assay (ELISA). 

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