Virus rabies

Yusibov et al. [71] delivered to 14 human volunteers spinach expressing epitopes from the rabies virus glycoprotein and nucleoprotein fused to the coat protein of alfalfa mosaic virus (AlMV). Five of the fourteen subjects had previously received a 

The conventional rabies vaccine was administered to the nine initially naive subjects seven days after completing the oral vaccination. Three of these volunteers produced neutralizing antibodies against rabies virus, although none of the five control subjects did. This study showed a clear indication that the orally delivered rabies vaccine has potential as an oral booster for the conventional rabies vaccine. 

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ToUwut, /. rabies, hydrophobia, -gift, n. rabies virus. 

In 1971, Hiatt et al. found that polyethylene oxide (PEO) of molecular weight about 100000 prevented the adsorption of rabies virus to porous glass with an average pore diameter of 1250 A. The support was modified by passage of one void volume of 0.4% solution of the polymer in water, followed by 5 or more volumes of distilled water or buffered salt solution. The virus was effectively purified from the admixtures of brain tissue fluid by means of size-exclusion chromatography on the modified glass column [28]. 

Pre-exposure immunization of people with greater than usual risk of exposure to rabies virus by reason of occupation (eg, veterinarians laboratory... 

Influenzavirus Rabies Virus Measies Virus Respiratory Synctiai Virus ... 

Rabiest Human diploid cell cultures infected with rabies virus 1 Clarification 2 Inactivation with beta-propiolactone 3 + 3 quantal assay in mice Inoculation of cell cultures to exclude live virus... 

Rabies Neutralization of the infectivity of rabies virus for mice Not less than 150111ml- ... 

The genes of the inducible and the constitutively expressed forms of NOS have been cloned and expressed. The expression of inducible NOS in the brain tissue of animals with experimentally induced neurological disorders (boma disease virus and rabies virus in rats), herpes simplex virus (mice) and experimental allergic encephalitis (in rats) suggests that NO produced by induced NOS may be a toxic fector in the pathogenesis of neurological diseases (Koprowski et /., 1993). 

Tobacco mosaic virus CP replacement, Second subgenomic promoter (sgp), CP fusion, Readthrough fusion with CP, fusion with cleavage site, fusion with CtxB malaria, rabies virus, M HV, FMDV, HCV, BHV-1, Ps. aeruginosa, neuropeptide, TMOF, allergens, MABs, a-trichosanthin, cytokines a-galactosidase-A, scFvs 11, 16,17, 52, 59-61... 

The sera of mice fed with fresh spinach leaves infected with AlMV particles presenting a rabies virus epitope contained IgG and IgA. Mucosal IgA was also detected [48]. Human volunteers (in FDA approved trials) fed with spinach containing recombinant particles generated both IgG and IgA responses specific to the pathogen [49]. The trials also suggested that plant virus particle-based vaccines could be effectively used in prime-boost regimens. In more recent work, recombinant AlMV particles containing an epitope from the G protein of human respiratory syncytial virus (RSV) induced protective immunity in mice [33]. 

Rabies virus glycoprotein (G)and nucleo-protein (N) Alfala mosaic virus and tobacco mosaic vims in tobacco and spinach leaf Elicited specific virus-neutralizing antibodies in mice. Immunogenic in mice when delivered orally and parenterally immunogenic in humans when delivered orally. Moderately protective against lethal challenge infection in mice. 16, 71... 

Rabies virus glycoprotein (G) Tomato leaf and fruit Intact protein forms. 108... 

Rabies virus protein Monoclonal antibody (mAb S057) Rabies virus neutralization N. tabacum cv Xanthi 35 S MSP +KDEL + 3 pg/g FLW (0.07% TSP) 33... 

CDC Case Definition Laboratory criteria for diagnosis is (1) a positive direct fluorescent antibody test (preferably performed on central nervous system tissue) or (2) isolation of rabies virus (in cell culture or in a laboratory animal). 

Material Safety Data Sheet-Infectious Substances Rabies Virus, Rabies-Related Viruses. March 5,... 

Inactivated rabies virus Live attenuated strains of rotavirus... 

Using the knowledge that rabies virus can spread into the brain neurons, scientists mimic its delivery system. A short, 29 amino acid peptide chain is derived from the rabies virus glycoprotein (RVG). The RVG binds to the acetylcholine receptor on the neurons and the endothelium cells of the blood-brain barrier. Through this interaction, transvascular delivery is enabled. 

Louis Pasteur (1864) discovered that microorganisms cause diseases, and he devised vaccination against rabies. This was achieved through the use of attenuated rabies virus. 

Rabies immunoglobulin Human Specificity against rabies virus... 

Rabies vaccines Inactivated rabies virus Active immunization against rabies... 

Monoclonal Antibodies Expressed in Plants against Rabies Virus... 

Ashraf, S., Singh, P.K., Yadav, D.K., Shahnawuz, M., Mishra, S., Sawant, S.V., and Tuli, R. (2005). High level expression of surface glycoprotein of rabies virus in tobacco and its immunoprotective activity in mice. J. Biotechnol. 119(1) 1-14. 

Yusibov, V., Modelska, A., Steplewski, K., Agadjanyan, M., Weiner, D., and Hooper, C. (1997). Antigens produced in plants by infection with chimeric plant viruses immunize against rabies virus and HlV-1. Proc. Natl. Acad. Sci. U.S.A. 94 5784-5788. 

Tobacco cell culture Anti-rabies virus monoclonal antibodies Girard et al., 2006 0.5 mg/L Disposable plastic bioreactor... 

Girard, L.S., Fabis, M.J., Bastin, M., Courtois, D., Petiard, V., and Koprowski, H. (2006). Expression of a human anti-rabies virus monoclonal antibody in tobacco cell cultnre. Biochem. Biophys. Res. Commun. 345(2) 602-607. 

Subunit vaccines Immunogenic proteins or peptide antigens customized to specific antigenic determinants are purified from tissue culture HIV, rabies virus, influenza virus, hepatitis B virus... 

Rabies virus Spinach Spike protein Modelska et ah, 1998... 

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