Rabies virus glycoprotein

Rabies virus glycoprotein (G)and nucleo-protein (N) Alfala mosaic virus and tobacco mosaic vims in tobacco and spinach leaf Elicited specific virus-neutralizing antibodies in mice. Immunogenic in mice when delivered orally and parenterally immunogenic in humans when delivered orally. Moderately protective against lethal challenge infection in mice. 16, 71... 

Rabies virus glycoprotein (G) Tomato leaf and fruit Intact protein forms. 108... 

Yusibov et al. [71] delivered to 14 human volunteers spinach expressing epitopes from the rabies virus glycoprotein and nucleoprotein fused to the coat protein of alfalfa mosaic virus (AlMV). Five of the fourteen subjects had previously received a... 

Using the knowledge that rabies virus can spread into the brain neurons, scientists mimic its delivery system. A short, 29 amino acid peptide chain is derived from the rabies virus glycoprotein (RVG). The RVG binds to the acetylcholine receptor on the neurons and the endothelium cells of the blood-brain barrier. Through this interaction, transvascular delivery is enabled. 

These are discussed elsewhere but include a recombinant vaccinia virus that expresses rabies virus glycoproteins used for oral vaccination. 

Dietzschold, B., Gore, M., Marchandiar, D., et al. (1990) Structural and immunological characterization of a linear virus-neutralizing epitope of the rabies virus glycoprotein and its possible use in a synthetic vaccine. J. Virol. 64,... 

Zhou D, Cun A, Li Y, Xiang Z, Ertl HC. A chimpanzee-origin adenovirus vector expressing the rabies virus glycoprotein as an oral vaccine against inhalation infection with rabies virus. Mol Ther 2006 14 662-72. 

B. S. Wojczyk, M. Stwora-Wojczyk, S. Shakin-Eshehnan, W. H. Wunner, and S. L. Spitahiik,The role of site-specific A-glycosylation in secretion of soluble forms of rabies virus glycoprotein, Glycobiology 8 (1998), 121-130. 

Cox JH, Dietzschold B, Schneider LG (1977) Rabies virus glycoproteins. II. Biological and serological characterization. Infect Immun 16 754-759. 

Mazarakis ND, Azzouz M, Rohll JB, El-lard FM, Wilkes FJ, et al. 2001. Rabies virus glycoprotein pseudotyping of lentiviral vectors enables retrograde axonal transport and access to the nervous system after peripheral delivery. Hum. Mol. Genet. 10 2109-21... 

Monoclonal antibodies for therapeutic and diagnostic use seem to be the best potential successful products produced in plants, but they currently have not reached the marketplace. Tobacco plants are the most commonly used plant in these developments. Hepatitis B surface protein and rabies virus glycoprotein are examples of advances in edible vaccines. 

Rabies Tobacco/spinach Rabies virus glycoprotein AMT 22... 

Figure 10.10 (a) In vivo near-infrared (NIR) fluorescence images of nude mice after intravenous injection of pluronic-based nanocarriers, (b) Ex vivo NIR fluorescence image of major organs B, brain S, spleen L, Aver H, heart Lm, lung K, kidney), (c) Quantification of the nanocarriers accumulated in the sacrificed brain, (d) Quantification of the nanocarriers distributed in the brain and major organs excised from the mice at 48 h post injection. ( = 3, p<0.01 Bare-NC, Chito-NC, RVG-Bare-NC vs. RVG-Chito-NC). Chito, chitosan RVG, rabies virus glycoprotein NC, nanocarrier. 

Gaudin Y, Ruigrok RW, Tuffereau C, Knossow M, Flamand A. Rabies virus glycoprotein is a trimer. Virology. 1992 187(2) 627-632. 

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