Cell transformation test

In Vitro Syrian Hamster Embryo Cell Transformation Test. 308... 

The in vitro mammalian cell transformation test EU Annex V B.21... 

Positive results from the in vitro mammalian cell transformation test indicate that the test substance produces phenotypic changes in cultured mammalian cells associated with malignant transformation in vivo. None of the in vitro test endpoints has an established mechanistic link with cancer. Some of the test systems are capable of detecting tumor promoters. 

Danford, N. (1991) The genetie toxicology of or//zo-toluidine. Mutat. Res., 258, 207-236 Daniel, M.R. Dehnel, J.M. (1981) Cell transformation test with baby hamster kidney cells. In de Senes, F.J. Ashby, J., eds, Progress in Mutation Research, Volume 1, Evaluation of Short-Term Tests for Carcinogens. Report of the International Collaborative Program, Amsterdam, Elsevier Seienee, pp. 626-637... 

Dana MT, Lee RN, Hales JM. 1985. Hazardous air pollutants Wet removal rates and mechanisms. Richland WA U.S. Environmental Protection Agency. EPA 600/3-84-113. NTIS PB85 138-626. Daniel MR, Dehnel JM. 1981. Cell transformation test with baby hamster kidney cells. Mutat Res... 

Daniel MR, Dehnel JM. 1981. Cell transformation test with baby hamster kidney cells. Mutat Res 1 626-637. 

Styles JA. 1981. Activity of 42 coded compounds in the BHK-21 cell transformation test. Prog Mutat Res 1 638-646. 

Quercetin, a flavonoid component of St. John s wort and several other medicinal plants, has been implicated as a mutagen. However, St. John s Wort aqueous ethanolic extract showed no mutagenic effects in mammalian cells. Tests used included the HGPRT (hypoxanthine guanidine phosphoribosyl transferase) test, the UDS (unscheduled DNA synthesis) test, the cell transformation test uring Syrian hamster embryo cells, the mouse fur spot test, and the chromosome aberration test using Chinese hamster bone marrow cells (Okpanyi et al., 1990). 

Diclobutrazol is of low toxicity to mammals and other animals. The acute oral and dermal lDjoS for rats are about 4000 mg/kg and > 1000 mg/kg, respectively. Ames and cell transformation tests gave negative results. Diclobutrazol is also of low toxicity to birds, fish and invertebrates. 

Styles JA. 1981. Activity of 42 coded compounds in the BHK-21 cell transformation test. In deSerres FJ, Ashby J, eds. Evaluation of short-term tests for carcinogens Report of the International Collaborative Program. Prog Mutat Res 1 638-646. 

Much interest is now being shown, at governmental level, in two tests that can give more rapid results than those done on rodents. These are (i) the Ames test which looks for mutagenicity in a susceptible bacterium (Ames, Sims, and Grover, 1972), and (ii) the Styles cell transformation test (Purchase et al. 1976). The former pronounces 2-naphthylamine positive but... 

---