Viruses pseudorabies

Li+ has significant inhibitory effects upon DNA viruses, in particular HSV which has been studied in depth. It was originally shown that Li+ inhibits viral replication in a dose-dependent, reversible manner in HSV-infected baby hamster kidney cells [240], and this has been found to be due to a Li+-induced decrease in the synthesis of viral DNA [241]. It is now well established that Li+ inhibits DNA synthesis in HSV types 1 and 2 and in several other DNA viruses, including measles, vaccinia, adenovirus, poxvirus, pseudorabies virus, Epstein-Barr virus, and the bovine, equine, and canine HV s [241]. Interestingly, Li+ has no effect on the replication of RNA viruses, such as influenza or encephalomyo-carditis virus. 

Aston-Jones, G. and Card, J. P. (2000). Use of pseudorabies virus to delineate multisynaptic circuits in brain Opportunities and limitations. J. Neurosci. Methods 103(1), 51-61. 

Card, J. P. et al. (1990). Neurotropic properties of pseudorabies virus Uptake and transneuronal passage in the rat central nervous system. J. Neurosci. 10 (6), 1974-1994. 

Husak, P. J., Kuo, T. and Enquist, L. W. (2000). Pseudorabies virus membrane proteins gl and gE facilitate anterograde spread of infection in projection-specific neurons in the rat. J. Virol. 74(23), 10975-10983. 

Herpes and pseudorabies viruses will infect BHK Cl 3 cells in suspension. 

For herpes and pseudorabies virus this assay is similar to the... 

After 2 h add heparin to the pseudorabies virus infected cultures. 

O Donnell P, Lavin A, Enquist LW, Grace AA, Card JP (1997) Interconnected parallel circuits between rat nucleus accumbens and thalamus revealed by retrograde transynaptic transport of pseudorabies virus. J Neurosci 77 2143-2167. 

Mucsi et al. [78] reported that trifluoperazine, chlorpromazine, and promethazine were effective against herpes simplex virus. Certain benzo[a]-phenothiazines also produced identical results against herpes virus [78]. Some structurally related phenothiazines exhibited photodynamic activity against the pseudorabies virus [33]. The inhibitory effect of the phenothiazines was investigated on the multiplication of several viruses. 

Warner MS, Geraghty RJ, Martinez WM, Montgomery RI, Whitbeck JC, Xu R, Eisenberg RJ, Cohen GH, Spear PG (1998) A cell surface protein with herpesvims entry activity (HveB) confers susceptibility to infection by mutants of herpes simplex vims type 1, herpes simplex virus type 2, and pseudorabies virus. Virology 246 179-189. 

Mettenleiter, T.C. New developments in the construction of safer and more versatile pseudorabies virus vaccines. Dev. Biol. Standardization 1995, 84, 83-87. 

The virus reduction studies of the three process steps discussed here were performed with HFV-l, Bovine viral diarrhea virus (BVDV), Pseudorabies virus (PRV), Reovirus type 3 (Reo), Hepatitis A virus (HAV), and Porcine parvovirus (PPV). HIV-1 was included as a relevant enveloped virus, while BVDV and PRV were tested as specific model viruses for HCV and HBV, respectively (Table 1). Reo was chosen as a non-specific model non-enveloped virus, HAV was included as a relevant virus and PPV was used as a surrogate for human parvovirus B19. All viruses were propagated using standard cell culture conditions. " The appropriate cell lines were infected, at a low multiplicity of infection, and incubated until 4-1- cytopathic effects were observed. The infected cells were frozen and thawed three times to release virus, centrifuged at low speed to remove cell debris and the clarified supernatants were removed for use as virus spikes. 

PRV porcine pseudorabies virus FVIII molecule, retaining the known func-... 

Process step Pseudorabies virus Xenotropic murine retrovirus Human adenovirus Porcine parvovirus Poliovirus Mouse adenovirus ... 

Platelet aggregating factor Panax ginseng saponins Panax notoginseng saponins Pseudorabies virus Polyovirus... 

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