Cell specific growth rate

If you aerate a bioreactor, power consumption is much less than a non-aerated bioreactor. Oxygen transfer rate, OTR 6 X 10 3 kgm-3 Oxygen uptake rate, OUR 0.65 mmol 02 kg 1 cell Specific growth rate, umax 0.5 h 1... 

By taking into account only CTC-positive cells in the estimation of cell specific growth rates, higher values were obtained for all bioassays as expected, and no difference between values in HFe and LFe conditions was observed (Table 2). BGE ranged from 0.05 (n.s. P > 0.05) to 0.30 (Table 4). LFe-HFe conditions also affected bacterial growth efficiency, particularly when organic matter was only provided in the dissolved form (Table 4). 

Specific growth rate of the producing strain is also a relevant parameter for enzyme production by fermentation. Many enzymes are synthesized as growth-associated metabolites so that cell specific growth rate has a direct impact on enzyme specific rate of synthesis as shown by the non-structured model of Luedeking and Piret (1959) ... 

Craistant feeding Constant feed of nutricaits with preset speed, cell-specific growth rate gradually decreased, cell density increases linearly... 

Figure 30. The cell specific growth rate, and fluxes for glucose, glutamine and lactate were correlated to heat flux at different dilution rates in a continuous culture (Reproduced from Reference [119] with permission).

Loop reactors are particularly suitable as bioreactors to produce, for example, single-cell protein (96). In this process, single yeast or bacteria ceUs feeding on methanol multiply in aqueous culture broths to form high value biomass at 35—40°C, 20 kg/m ceU concentrations, and specific growth rates of... 

A plot of the specific respiration rate ( 02 versus the specific growth rate coefficient [L is linear, with the intercept on the ordinate equal to the oxygen uptake rate for cell maintenance. A formulation of this is ... 

Flow rate) (Cell in Cell out) + Rate of cell generation = 0 Let us define /a, which is known as specific growth rate... 

Table 3.1 shows the kinetic parameters for cell growth, rate models with or without inhibition and mass transfer coefficient calculation at various acetate concentrations in the culture media. The Monod constant value, KM, in the liquid phase depends on some parameters such as temperature, initial concentration of the carbon source, presence of trace metals, vitamin B solution, light intensity and agitation speeds. The initial acetate concentrations in the liquid phase reflected the value of the Monod constants, Kp and Kp. The average value for maximum specific growth rate (/xm) was 0.01 h. The value... 

There is no cell removal from the batch vessel and the cell propagation rate is proportional to specific growth rate, /jl (h 1), using the differential growth equation the cell concentration with respect to the time is ... 

The effect of substrate concentration on specific growth rate (/i) in a batch culture is related to the time and p,max the relation is known as the Monod rate equation. The cell density (pcell) increases linearly in the exponential phase. When substrate (S) is depleted, the specific growth rate (/a) decreases. The Monod equation is described in the following equation ... 

A Monod rate model is used to demonstrate the rate of biomass generation. We neglect the cell death rate. Let us denote the ratio of biomass rate of generation to biomass concentration, rJX, that is the specific growth rate /a also denotes the dilution rate D is defined as number of tank volumes passed through per unit time, FIV. After substitution of D and /a into (6.8.1), the following equation is obtained ... 

The Monod rate model is valid for a CSTR bioreactor with maximum specific growth rate of 0.5 li 1 and K, 2 g-1. What would be a suitable dilution rate at steady-state condition, where there is no cell death if initial substrate concentration is 50g-l-1 and yield of biomass on substrate is 100%. 

The parameters of the Monod cell growth model are needed i.e. the maximum specific growth rate and the Michaelis-Menten constant are required for a suitable rate equation. Based on the data presented in Tables 10.1 and 10.2, obtain kinetic parameters for... 

The effect of a particular cultivation environment on a system can be evaluated in terms of biomass (fresh/dry weight, cell number), secondary metabolite production [51,75,89,102,103,106,107] or substrate consumption (e.g. carbon source [57] or oxygen [53,108]). Using the Evan s Blue method to identify non-viable cells. Ho et al. [108] used viable cell density measurements to determine variations in specific growth rate attributable to hydrodynamic stress. 

Figure 1 shows that high superficial gas velocity in the airlift bioreactor obviously inhibited growth of H. pluvialis. In this study, the best superficial gas velocity for growth of H. pluvialis was found at the lower limit of the experiment (0.4 cm s ), which providing the maximum cell density and maximum specific growth rate of 77x10 cells mL (2.79 g L of... 

When microorganisms use an organic compound as a sole carbon source, their specific growth rate is a function of chemical concentration and can be described by the Monod kinetic equation. This equation includes a number of empirical constants that depend on the characteristics of the microbes, pH, temperature, and nutrients.54 Depending on the relationship between substrate concentration and rate of bacterial growth, the Monod equation can be reduced to forms in which the rate of degradation is zero order with substrate concentration and first order with cell concentration, or second order with concentration and cell concentration.144... 

The maximum specific growth rate pm, Monod constant Km, and cell yield coefficient Y are constants. In (E4-33), C2o is the inlet glucose concentration. 

The slope of the curve in the limitation region decreases as it approaches t2. The slope represents the growth rate per unit mass of cells or specific growth rate and is given the symbol /x (s 1), where ... 

Thus, the specific growth rate in a chemostat is controlled by the feed flow rate, since // is equal to D at steady state conditions. Since ft, the specific growth rate, is a function of the substrate concentration, and since fi is also determined by dilution rate, then the flow rate F also determines the outlet substrate concentration S. The last equation is, of course, simply a statement that the quantity of cells produced is proportional to the quantity of substrate consumed, as related by the yield factor Yx/s-... 

Once the cells have adjusted to growth in the new environment the specific growth rate (h-i) of the organism will gradually increase up to a maximum valued (when At is... 

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