Vials

Both the conical vials (3 and 5 mL) and the round-bottom flasks are designed to be connected via an O-ring compression cap installed on the male joint of the adjacent part of the system (see Fig. 3.1). 

Two types of condensers (air condensers and water-jacketed condensers) are available in most cases the water-jacketed condenser can work well as an air condenser. Condensers are usually attached to 14/10 I-jointed reaction flasks. The upper female joints allow connection of the condenser to the 14/10 1 drying tube and the 14/10 I capillary gas delivery tube (see Fig. 3.2). 

These are small glass bottles which are fitted with a ventilated stopper which can be partially seated into the vial neck to permit the escape of water vapour during drying or fully inserted at the end of the process to seal the vial prior to its removal from the freeze-drier. Vials are perhaps the most convenient container used for freeze-drying parenteral products. 

These have been developed to combine the convenience of the vial with the sealing qualities of an ampoule and can be temporarily stoppered within the freeze-drier prior to final heat sealing.These special ampoules are particularly unstable, presenting problems during filling or sealing which have limited their application. 

These provide a safer alterative to glass containers although limited availability, the need to use specialist filling and sealing equipment and the requirement to conform to regulatory pharmaceutical requirements has restricted their use. 

These contain both freeze-dried product and rehydrating fluid and reduce the risk of sample/diluent mismatch and contamination during sample reconstitution. 

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A laboratory capped glass vial with a F shaped iron wire attached to its inner side by plasticine, (see Fig. 7), was tomographed with 80 keV. 

Figure 7. Test object no. 2, capped glass vial with an iron wire on the inside.

In figure 8(a) the top of the cap wasn t reconstructed well enough due to the same reasoning as in object no. 1. A similar deficiency is caused by a non sufficient number of exposure angles. Figure 8(b) shows the horizontal edge of the caps bottom. In Fig. 8(c) the side of the vial is clearly seen, yet with the reconstruction distortion pointed out for object 1. Figure 9... 

Flat-bottomed flask (the so-called Florence flask) with vial mouth. The sizes vary between 50 ml. and 40 litres. 

Anodic stripping voltammetry at a mercury film electrode can be used to determine whether an individual has recently fired a gun by looking for traces of antimony in residue collected from the individual s hands, fn a typical analysis a sample is collected with a cotton-tipped swab that had been wetted with 5% v/v HNO3. When returned to the lab, the swab is placed in a vial containing 5.00 mb of 4 M HCl that is 0.02 M in hydrazine sulfate. After allowing the swab to soak overnight,... 

Procedure. Samples are collected in 40-mL vials with screw-caps lined with a Teflon septum. Fill the vial to overflowing, ensuring that there are no air bubbles. Add a reducing agent of ascorbic acid (25 mg/40 mL) to quench the further production of trihalomethanes, and seal the vial. Store samples at 4 °C, and analyze within 14 days. 

Samples and calibration standards are prepared for analysis using a 10-mL syringe. Add 10.00 mL of each sample and standard to separate 14-mL screw-cap vials containing 2.00 mL of pentane. Shake vigorously for 1 min to effect the separation. Wait 60 s for the phases to separate. Inject 3.0-pL aliquots of the pentane layer into a GC equipped with a 2-mm internal diameter, 2-m long glass column packed with a stationary phase of 10% squalane on a packing material of 80/100 mesh Chromosorb WAW. Operate the column at 67 °C and a flow rate of 25 mL/min. 

Although chloroform is an analyte, it also can be interferent. Due to its volatility, chloroform present in the laboratory air may diffuse through the sample vial s Teflon septum, contaminating the samples. How can we determine whether samples have been contaminated in this manner ... 

Why is it necessary to collect samples such that there is no headspace (layer of air overlying the liquid) in the sample vial ... 

The basic instrumentation for capillary electrophoresis is shown in Figure 12.41 and includes a power supply for applying the electric field, anode and cathode compartments containing reservoirs of the buffer solution, a sample vial containing the sample, the capillary tube, and a detector. Each part of the instrument receives further consideration in this section. 

Injecting the Sample The mechanism by which samples are introduced in capillary electrophoresis is quite different from that used in GC or HPLC. Two types of injection are commonly used hydrodynamic injection and electrokinetic injection. In both cases the capillary tube is filled with buffer solution. One end of the capillary tube is placed in the destination reservoir, and the other is placed in the sample vial. 

Hydrodynamic injection uses pressure to force a small portion of the sample into the capillary tubing. To inject a sample hydrodynamically a difference in pressure is applied across the capillary by either pressurizing the sample vial or by applying a vacuum to the destination reservoir. The volume of sample injected, in liters, is given by the following equation... 

Electrokinetic injections are made by placing both the capillary and the anode into the sample vial and briefly applying an electric fleld. The moles of solute injected into the capillary, nj, are determined using... 

Potency of hGH preparations is quantitatively deterrnined, in terms of mass per vial, by one or more chromatographic procedures (50). Biopotency is calculated from the mass-based potency using a conversion factor, typically 3 lU/mg. Traditionally a bioactivity assay using hypophysectomized rats has been used to determine potency however, the imprecision of this assay has resulted in its use only as a semiquantitative indicator of bioactivity (1), sometimes referred to as a bioidentity test. 

Airlines, such as Air New Zealand and Virgin Air, give their long-distance passengers after-flight regulator travel kits, which contain vials of scents... 

Commercially available containers for use with parenteral products include single-dose ampuls that are heat sealed and opened by snapping at the point of least diameter, vials for multidose use, and botdes and pHable bags that are used for large volumes such as needed in intravenous infusions. Container size can vary from 1 mL to 1 L. Generally volumes up to 100 mL are available as ampuls or vials. 

Clinical Use. Vancomycin and teicoplanin as fomiulated dmgs are lyophilized powders to be reconstituted with sterile water for injection. Vancomycin hydrochloride [1404-93-9] is presented in vials of 500 mg that give 100—200 mL solution of pH 2.5—4.2. It is administered by slow (60 min) infusion at a dose of 500 mg every 6 h or 1 g every 12 h/d. The teicoplanin contains the five factors (87%) plus 13% of the pseudoaglycone T-A3-1. It is presented in vials containing 200 mg of lyophili ed power that after dissolution with 3 mL of solvent gives a solution at pH 7.5. The dose regimen is 200—800 mg/d by iv bolus adrninistration. 

Kits. Kits for the preparation of radiopharmaceuticals are a convenient solution to synthesis of products containing short-Hved radionucHdes (eg, In, I, Tc) bound to a nonradio active moiety. The labeling step is performed either at a commercial radiopharmacy, or within the institutional nuclear medicine laboratory. The kits are usually stored as a frozen solution or lyophilized product. The material of interest is then metered out into kit dosages. The kit vials are thawed or reconstituted and mixed with the appropriate radionucHde. 

Technetium-99m oxidronate is a bone imaging agent used to demonstrate areas of altered osteogenesis. It is rapidly cleared from the blood and taken up in areas of bone that are undergoing osteogenesis. The kit is a vial containing a lyophilized powder where sodium oxidronate is the active... 

Technetium-99m mertiatide (A/-[Ai-[A/-[(benzoylthio)acetyl]glycyl]glycine) is a renal imaging agent. It is excreted by the kidneys via active tubular secretion and glomerular filtration. The kit vial is reconstituted by using 740—3700 MBq (20—100 mCi) of Tc pertechnetate and boiling for 10 minutes. 

Technetium-99m mebrofenin is an iminodiacetic acid derivative used as a hepatobiliary agent. The kit is suppHed as a single vial containing lyopbilized mebrofenin. The reconstituted kit has 18-hour usage, owing to the preservative, propylparaben. 

Technetium-99m exametazime [(RR,3 3)-4,8-diaza-3,6,6,9-tetramethylundecane-2,10-dionebisoxime] is used as an adjunct in the detection of altered regional cerebral perfusion in stroke. The kit for the preparation of the radiopharmaceutical is suppHed as a single dose vial. 

Calcitonin. Calcitonin is available commercially from pork and salmon extracts (Calcimar, Armour) as well as by synthesis. Preparations are bioassayed on the basis of their calcium-lowering activity in comparison to the potency of pure pork calcitonin of which ca 4 p.g is equivalent to 1 MRC unit (Medical Research Council, U.K.). For clinical use, vials containing 400 units in 4 mL are available. The recommended daily dosage is 100 units to be adrninistered subcutaneously or intramuscularly because its plasma half-life is short (4—12 min). 

Composition and Methods of Manufacture. Vaccine is produced from the Oka attenuated strain. Vacciae is produced in human diploid cells such as MRC-5. After growth in the cell substrate, the cells themselves are harvested into the growth medium and sonicated to release the cell-associated vims. Sucrose and buffering salts are generally in the medium to help stabiLize the vims. The vacciae is presented in a free2e-dried vial to be reconstituted with sterile distilled water before injection (27). 

Containers Containers for hquids consist principally of drums, pads, and cans made of steel or plastic and of bottles and vials made of plastic or glass. The chemical industiy is often involved with all these containers, but the most frequently used packages for industrial chemicals are steel drums ana pails. For exotic products, stainless-steel drums and pails are available. The most common types used are 208-L (55-gal) drums and 19-L (5-gal) pails. 

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