Vial , freezer

To fortify a sample, the label from a fortification sampling vial was removed and secured to the pre-labeled sample jar. Spike vials were individually shaken before use. The cap was discarded, the contents of the vial were poured into the sample jar and then the vial was dropped into the sample. The sample jar was capped with a Tefion-lined lid, hand shaken to mix, placed in a Kapak bag and sealed. Jars were placed immediately in storage freezers. In all cases, quality control samples were transported and stored with their corresponding field plot samples throughout sample handling and shipment to the analytical facility. 

The vials are then transferred into freezer boxes and stored in the Liquid Nitrogen storage tank the next day. 

Remove vials of reagent from freezer, tear off metal seal, and gently remove the stopper. 

NOTE Solution of labeled standards stored in the freezer should be brought up to room temperature before opening the vials and using them. If standard solutions are in a very low-boiling solvent, e.g., pentane, use them refrigerated to facilitate pipetting. 

Appropriate safety procedures must always be followed when dealing with LN2 storage. Cells can be stored in liquid phase or vapour phase, or in ultracool freezers. Prevention of ice-crystal formation is essential for successful long-term storage and the vials should be protected from temperature fluctuations wherever possible. Temperature fluctuations can damage cells when frozen, even if they do not result in the contents of the vial thawing out. 

Place the vials were placed in a Nalgene freezing tub filled with isopropanol then place in a -70°C freezer overnight (see Note 24). 

Storage of solvent extracts or sorption tubes with analytes trapped, in freezer in glass vials closed with PTFE stoppers 1 month 115,116... 

When the samples are frozen (at — 70°C or — 120°C) they should be transferred to a liquid nitrogen freezer. These are of two types. In the older version the vials are actually immersed in liquid nitrogen but in the newer models the samples are simply held in the vapour above a sea of liquid nitrogen. 

For the liquid phase freezer, ampoules are transferred to canes (Fig. 7.5) or cryoboxes and immersed CAREFULLY into the liquid nitrogen vat. Gloves and goggles or visors must be worn to protect yourself from splashes of liquid nitrogen. Canes have the problem that vials can be lost from them or are difficult to remove when required, and the locator system of cryoboxes (Thermolyne Appendix 3) has advantages. 

Good records are important, otherwise freezers become filled with numerous vials of unknown provenance and the use of cells for commercial purposes requires a complete history of each vial. 

The methyl esters should be analyzed as soon as possible. They may be kept in an atmosphere of nitrogen in a screw-cap vial at 2° for 24 h. For longer storage, they should be sealed in a glass ampule, subjected first to a vacuum and then backfilled with nitrogen and stored at -20° (freezer). 

Tetrabenzyl pyrophosphate should be stored in a freezer as a dry solid. Stability data on TBPP are tabulated below [reported in Liquid Chromatography Area Percent (LCAP)]. The only impurity detected was dibenzyl phosphate. For this study, solid TBPP was sealed in scintillation vials with Parafilm, which were in turn enclosed in desiccators with indicating Drierite. 

Active concrete powder ( 9 kBq of tritium) was placed in a sealable polythene bag and put in one of eighteen Kilner jars which had very effective rubber lid seals. An open and a closed scintillation vial containing 10 ml each of RO water were added to each of the jars which were then stored in a fridge, a freezer and at room temperature for 1 day, 5 days, 10 days, 15 days, 20 days and 30 days, respectively. At the end of each pre-defined emanation time the respective Kilner jars were opened and the contents removed. 8 ml of each RO water vial (open and closed) was taken and transferred to clean scintillation vials with 12ml of scintillation cocktail (Goldstar) and counted by LSC for 1 hour. Similarly 50 ml of tritium-free RO water was added to the Kilner jar and shaken to collect any tritium (as HTO) that had adsorbed on the walls of the glass jars. An 8ml sample of this was also mixed with scintillant and counted. 

Active desiccant (nominally 170 kBq tritium) was placed in each of twelve sealable plastic boxes having an 0-ring lid seal. Samples of (i) silica gel in sealable polythene bag, (ii) silica gel in a double sealed bag, (iii) silica gel in an open scintillation vial and (iv) cellulose filter paper in an open scintillation vial were individually placed in each of the boxes. These were stored for 2 weeks in a fridge, a freezer and at room temperature before opening, sampling and measuring. 

Figure 2. Variations of cross contamination of tritium from concrete (--9 kBq/g) with the passage of time (Expt. 1). RO water in open and closed scintillation vials were stored in airtight Kilner Jars with a bagged sample of active concrete at room temperature, in a fridge and in a freezer for 1 day, 5 days, 10 days, 15 days, 20 days and 30 days.

Contamination of desiccants exposed to tritiated concrete was significantly reduced by storing the samples in a freezer or within sealed vials. 

This method uses a turret with multiple ports (a manifold) to which flasks and vials are fitted via suitable valves (see Fig. 2.3). The product is either frozen in a freezer (by direct submersion in a low-temperature bath) or shelf-frozen, depending on the nature of the initial and end product, and also on the volume to be freeze-dried. The pre-frozen product must be immediately attached to the drying chamber or manifold to prevent... 

So far, it has not been possible to preserve the activated component II by freeze-drying. Therefore, it is desirable to prepare the activated component II at the time of use and to store it in the low temperature freezer (-80 °C) in vials. A gel-filtration pattern of trypsinized component II on Sephacryl S-300 and SDS-PAGE of the fractions are shown in Figure 1 in this case, one protein peak of activated component II was obtained. 

Unopened insulin vials must be temperature-controlled until needed. Once opened, the insulin vial can be kept at room temperature for 1 month or in the refrigerator for 3 months. Do not put an open insuhn vial in the freezer. Do not place in direct sunlight or a high-temperature area. 

Freeze slowly by adding vials to either a standard freezer box that has been packed with cotton or a commercially available ethanol freezing container and leave it in a -70°C freezer overnight. 

Shock-freeze the solution by carefirlly dipping the vial into an ethanol/dry ice-bath, and store in a -80°C freezer until the freeze drying process is to commence. 

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