Sterile distilled water

Composition and Methods of Manufacture. Vaccine is produced from the Oka attenuated strain. Vacciae is produced in human diploid cells such as MRC-5. After growth in the cell substrate, the cells themselves are harvested into the growth medium and sonicated to release the cell-associated vims. Sucrose and buffering salts are generally in the medium to help stabiLize the vims. The vacciae is presented in a free2e-dried vial to be reconstituted with sterile distilled water before injection (27). 

A 100 ml aliquot of this medium is placed In a 500 ml Erlenmeyer flask and sterilized by autoclaving for 20 minutes under 15 psi pressure. Spores of mutant strain S. aureofaciens S1308 (ATCC No. 12,748) are washed from an agar slant Into the flask with sterile distilled water to form a suspension containing approximately 10 spores per milliliter. A 1.0 ml portion of this suspension is used to inoculate the fermentation media in the example which follows. A fermentation medium consisting of the following ingredients was prepared. 

The spores from an inclined culture of Fusarium lateritium Wr, CSB 119.63 on a gelose medium are extracted with sterilized distilled water to obtain a suspension containing about 600,000 spores per ml. This suspension is then used to seed the medium prepared as earlier described. The contents of the flask are left to incubate at 27°C. Sterile air is injected into the liquid to effect thorough agitation and uniform supply of oxygen into the medium. 

Then, 1.3 ml of glycerine are mixed with 0.5 ml of a 25% solution of methyl p-hydroxy-benzoate in ethanol, and 50 ml of distilled water are added. To the produced mixture are, after sterile filtration, added 10 ml of the stock solution 1, 2.5 ml of the stock solution 2 and 10 ml of the stock solution 3, after which 3.0 ml of sterile 0.1 N sodium hydroxide are added, and the mixture is filled up with sterile distilled water to a volume of 100 ml. The insulin will be precipitated amorphously by the admixture of the sodium hydroxide, and the produced suspension acquires the pH value of 7. It will contain approximately 1 gamma zinc per insulin unit. 

Chlorox) for 20 min. Winterbuds were then rinsed 3 times with sterile distilled water to remove the hypochlorite. Sago pondweed "tubers" were rinsed in distilled water, but not surface sterilized. 

Barbula convoluta, Tortella flavovirens and Pleurochaete squarrosa, spp. (that only sporadically produce sporophytes), were collected and cleaned from dust and dead parts, thoroughly washed in tap water and then in solution of Triton X-100 (0.8%), rinsed in distilled water and surface sterilized for 10-20 seconds in 1% sodium hypochlorite solution and rinsed four times in sterilized distilled water. 

White trap (White 1927) is one of the most common methods to produce entomopathogenic nematodes. Insects are inoculated with entomopathogenic nematodes on a petridish lined with filter paper. After 2-5 days, the infected insects are transferred to the White trap. The White trap consist of an inverted watch glass placed in a petridish on which Whatman paper of appropriate size is placed and moistened with sterilized distilled water. Adequate amount of distilled water is also maintained on and around the watch glass. As the infective juveniles emerge from the cadaver they migrate to the surrounding water and get trapped. The nematodes are harvested from the White trap and collected in a beaker. The concentration of nematodes can be accomplished by... 

Each flask colonized with the bacteria is inoculated with surface sterilized 500-1,000 infective juveniles of an appropriate species in 5 ml sterilize distilled water and are incubated at 25°C. The flask after inoculation should not be shaken vigorously to enable better feeding and reproduction of the nematode. 

After the experiments, the Prototype Unit was disassembled and the component parts were individually swabbed with sterilized cotton wools (4 cm2). Each samples were stored in 1 ml sterilized distilled water in an eppendorf tube. 50 pi of sample was transferred to TSA and MEA plates. The TSA plates were incubated at 37 °C for 24 h and bacterial colonies were counted. The number of fungal colonies was determined from the MEA plates after incubating at 30 °C for 5 days. The results show no viable bacterial and fungal colonies were present in the interior parts of the Prototype Unit. Viable colonies are found on the external surface of the unit. This suggests that air passing through the Prototype Unit was sterilized by the action of the formulated catalyst. 

Several features of the PCBB experiments are different than those for chlorpyrifos. The hydrolysis reaction proceeds via a different mechanism. The rate enhancements observed for chlorpyrifos in natural waters and the aqueous phases of the sediment/water systems (as compared to sterile distilled water) are not observed for PCBB. The values of kj and k calculated for PCBB are slower than those for chlorpyrifos anS similar in magnitude to the hydrolysis rates. 

Test Procedure. The test organisms were subcultured onto Tryptone Soya Agar (TSA) slopes and incubated at 37 °C for 24 hours. After incubation for each inoculum, 6 ml sterile distilled water (SDW) was added to each slope in turn, the organisms washed off and the resultant suspension homogenised. A 1 ml aliquot of the suspension was added to 100 mis SDW and homogenised to form the inoculum. 

Dissolve the pellet in sterilized distilled water and quantitate the DNA concentration by spectrophotometer, then use as the template for mRNA synthesis (tee Note 12). 

Procedures to set up the NICK Columns are as follows. First, remove the column cap and pour off the excess liquid. Rinse the column with 3 mL ofsterilized distilled water. Remove the bottom cap and place it in a column stand. Equilibrate the gel with 3 mL of sterilized distilled water and flush completely. These procedures should be carried out during the in vitro transcription reaction. 

Apply 100 pL of the transcriptional reaction mixture on top of the gel, and flush completely. If the reaction scale of the in vitro transcription is less than 100 pL, fill up the reaction mixture to 100 pL with sterilized distilled water before applying to the column. 

Add 400 piL of sterilized distilled water, and then flush completely. Discard the eluate. 

Mix 8 pg of the mRNA sample and 11 pL of the RNA sample buffer, and adjust to 20 pL with sterilized distilled water. 

Sterile distilled water Sterile screw-cap test tubes Sterile buffer (dilution blanks)... 

The British Pharmacopia states, Water for injections is sterilized, distilled water, free from pyrogens. It is obtained by distilling potable water, purified water or distilled water. ... 

Bacillus Calmette-Guenn (BCG)- An attenuated strain of bovine tubercle bacillus is available from Glaxo or from outdated hospital supplies as a lyophilized powder. Before use, it is suspended in sterile distilled water. 

Phosphate-buffered saline (PBS), pH 7.4. 8.0 g of NaCl, 0.2 g of KH2P04, 2.9 g of Na2HP04 12H20, 0.2 g of KC1, and 0.2 g of NaN3 Make up to 1 L with sterile, distilled water. Alternatively, PBS tablets can be obtained from Sigma or Oxoid, Unipath Ltd, Basingstoke, UK Each tablet is dissolved m a known volume (as recommended by suppliers) of sterile, distilled water. Store at room temperature. 

Glycogen, mussel glycogen at 20 mg/mL in sterile distilled water. 

TE buffer Dissolve 10 mM Tris-HCl and 1 mMEDTA (pH 8.0) in sterile distilled water Do not autoclave. 

Ethidium bromide (EtBr) Dissolve 10 mg of EtBr m sterile distilled water. Dilute to 0 5 pg/mL of distilled water for staining the gel EtBr is a suspected carcinogen Handle with care in a fumehood. 

X Denhardt s solution- 0.2 g BSA, 0 2 g ficoll, 2 g glycine, 0.2 g polyvinyl pyrrolidone, sterile distilled water to 10 mL. Store m aliquots at—20°C... 

Human placental DNA. 10 mg/mL in sterile distilled water. Stir for 1-2 h, shear by passing through 18-gage needle, or sonicate Place m boiling water bath for 10 min Store m aliquot at —20°C... 

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