Glass scintillation vials

Dry the appropriate amounts of each radiolabel (usually supplied in ethanol or toluene) under a stream of N2 in a glass tube or glass scintillation vial. Typically, the non-absorbed marker is used at 0.2-0.5 pCi per mouse and the cholesterol at 0.5-1.0 pCi per mouse. 

For those interested in cloning reference should be made to Chapter 7. A small scale perfusion vessel is considered in 3.4.3. For many biochemical studies involving incubation of cells with radioisotopes in the presence of drugs, anti-metabolites, hormones etc. small numbers of cells are required and these may conveniently be grown on the bottoms of glass scintillation vials or in the wells of a 6 or 24 well TC plate or even in the wells of a microtitre plate (see Table 3.1). This last method enables 96 replicate cultures to be handled simultaneously but the maximum volume that each well will hold is 0.25 ml. 

Set up cultures of hamster CHO cells in glass scintillation vials (2 X 105 cells in 1 ml of Eagle s MEM supplemented wth 10% calf serum, hypoxanthine (30/rM) and glycine (IOOjuM), and buffered with Hepes buffer. 

Figure 9 Transmittance of a Pyrex Petri Dish cover compared to a 20 ml borosilicate glass scintillation vial.

Figure 10 Transmittance of plastic wraps and a 20mL borosilicate glass scintillation vial. Source Courtesy of Kimble Glass, Vineland, NJ, U.S.A.

Figure 15 Illustration of a 20mL borosilicate glass scintillation vial with different sample volume, cover, and orientation used during a photostability study.

The entire assay can conveniently be carried out in a glass scintillation vial [82]. The reaction volume contains Tris buffer (pH 7.5), Mg, [ H]cyclic nucleotide, and snake venom 5 -nucleotidase. After a 10-min incubation at 37°C, the reaction is stopped by the addition of a slurry of AG1-X2 anion exchange resin. The resin binds substrate cyclic nucleotides but does not bind nucleosides. Scintillation fluid is added and the amount... 

Analyses. Liquid scintillation counting was used to measure the Pu activity in all samples. Aqueous samples (1.00 or 2.00 ml) were added to 18 ml of Insta-Gel scintillation cocktail (Packard Instrument Co.) in glass scintillation vials and thoroughly mixed. A Packard Tri-Carb Model 1550 liquid scintillation analyzer was used to count all the samples. 

Incubate the sections in glass scintillation vials (or similar containers) containing the primary antibody (diluted in PBS-BSA-TX-1.0) for 12-48 h (see Notes 29 and 30). 

The standard ( H)protein gels and the electrophoresed gels were sliced and each slice was transferred to a glass scintillation vial. They were then handled in four different ways. 

In a darkened room with a photo red safety light, 2.5 ml of dark-adapted RPMI-PS and 2.5 ml of the above PBMC suspension are added to glass scintillation vials (20 ml, Kimble,... 

The samples were absorbed in 9 ml of Carbo-Sorb and collected in low potassium glass scintillation vials. To this were added 13 ml of homogeneous blend toluene based scintillator (Permafluor V ). Permafluor V contains 91% PPO (2,5 diphenyloxazole) as the primary scintillator and 9% POPOP (l,4-bis-2(5-phenyloxazolyl)-benzene) as the secondary scintillator. ... 

Elute. Prior to elution the dots are cut from the membrane sheet. Elution (45 min in 2 ml of elution buffer) is performed in glass scintillation vials on a laboratory shaker at ambient temperature (bright illumination should be avoided). Dried sheets have to be rewetted in destaining solution prior to immersion in elution buffer. It is recommended that the destaining solution (25 pi) and the elution buffer be dispensed with appropriate repetitive devices (e.g., Eppendorf Multipette and Brand Dispensette, respectively). 

After sampling, the filters are carefully removed from the cassettes and individually transferred to small vials containing approximately 2 mL deionized water. The vials must be tightly sealed. The water can be added before or after the filters are transferred. The vials must be sealable and capable of holding at least 7 mL of liquid. Small glass scintillation vials with caps containing Teflon liners are recommended. 

Before use the silicon shards must be cleaned see Note 1). To do this, the shards are placed in a glass scintillation vial and covered with approximately 10 ml of HPLC grade hexane see Note 2). The vial is placed in a bath sonicator for 10 min. Drain the hexane and repeat. A third rinse using absolute ethanol should be conducted in the same manner. 

Glass Scintillation vials shonld not be capped as solvent vapor can extract polymers from the cap, contaminating the solvent. 

Glass scintillation vials with foil lined caps, or 10-mL Reacti-Vials (Pierce) with Teflon-lined rubber-cap liners... 

In two bioassays, semiochemicals were applied first to the bottom and sides of glass scintillation vials, while in the second, an equivalent quantity was applied to the inside of the top of the vial. In both cases, the semiochemicals were applied in solvent, then the solvent was allowed to evaporate. Surfaces or lids of control vials were treated with solvent only. After the solvent had evaporated, five neonate larvae of the variegated cutworm, Peridroma saucia, were introduced into each vial for a 4-h exposure period. After exposure to the test chemicals, larvae were transferred to artificial diet, and their survival was assessed 24 h later. Because larvae could not climb the slippery surfaces of the glass vials and contact the... 

The LSC measurements were carried out with the TriCarb 3170 TR (PerkinElmer) in the low-level mode. High performance (low potassium) glass scintillation vials from PerkinElmer were used throughout. The temperature in the LSC measurement chamber was monitored continuously to ensure that all measurements were carried out in the range 13 °C ( 2 °C). To further minimize quench variability, the additions of scintillation cocktail (InstaScintGel Plus), SrCOs solubilizer (aqueous toluene sulfonic acid), carriers (Sr and Y) and water were maintained constant in all sample, standard and background vials. The standard conditions for measurement are given in 2.3. 

Glass scintillation vials (20 ml) or Eppendorf tubes for fixation and devitellinization Pasteur pipette... 

Weigh 4 equivalents of Boc-protected amino acid into a 25 mL sealable glass scintillation vial. Add the required a volume of the HBTU stock solution corresponding to the correct stoichiometric ratio. Close the vial and shake the suspension until the AA is completely dissolved. 

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