Conical vials

Autosamplers take this same loop and valve principle and automate the filling and handle-turning sequence. The major differences between models on the market are in the way they get sample into the loop and the method of cleaning between injections. Most autoinjectors use a carousel loaded with sample valves to hold samples until their turn for injection occurs. Sample vials are usually capped with a screw cap fitted with a septum, although some recent autosamplers replace the carousel with microtiter plates having 96-364 wells containing the samples for use with robotic workstations. Conical vials are available for limited samples and 1-jUL injections are possible with some... 

Evaporate the toluene fraction from Paragraph 9.10.5 to approximately 1.0 mL in a rotary evaporator (see Section 9.8). Transfer the extract to a 2.0 mL conical vial using a toluene rinse. 

Dilutions are performed using an aliquot of the original extract, of which approximately 50 pL remain from Paragraph 9.11.2. Remove an appropriate size aliquot from the vial and add it to a sufficient volume of tridecane (or nonane) in a clean 0.3 mL conical vial. Add sufficient recovery standard... 

Into a dry, 50-mL, conical vial is placed dimethyl diazomalonate (29.4 mmol, 4.65 g) and 25 mL of chloroform (Note 9). The solution is taken up into a syringe (Note 10) fitted with a 30-cm needle. The needle is inserted about half way into the septum on the stopcock so that the needle tip extends down into the condenser. The syringe is placed on a syringe pump (Note 11) which is set to add the solution at a rate of 1.17 mL/hr over a period of about 25 hr. After the addition is complete, the syringe is again filled with 2 mL of CHCI3, which is added to the system via the septum. The needle Is... 

Use equation (6.4) M-(V, = MfVf) to determine the volume, x ml, of your stock to be diluted into a-MEM. Prepare this diluted solution in a 50 ml sterile conical vial. 

Prepare a concentrated Stock A (3.0 mg/100 pi) of cw-platin from which Stocks B-I will be prepared Table 6.5 details the preparation of your Stocks for this experiment. To efficiently prepare your solutions in a timely fashion to minimize DMSO exchange (see Experiment 6.2), label 11 150 pi conical vials A-K. Add the appropriate volume of DMSO solvent indicated in column 6 to each vial. Prepare Stock A. Quickly make serial dilutions as indicated in Table 6.5, vortexing for rapid mixing (recall the rapid substitution kinetics of cwplatin in DMSO—Experiment 6.2 ). Note that the drug STOCK labels are identical to the well labels. After the addition of drug and medium the total volume in each well is 3.5 ml. 

MTT Assay MTT solutions are prepared by dissolving 250 mg MTT in 100 ml of a 50 50 mixture of PBS ti Iter sterilized ddH20. Partition the solution into 10 ml portions in 15 ml conical vials. Wrap in foil and store at 4 °C until needed. 

Drain the solution into a 50 ml conical vial that has been cooled on ice. 

Complete the transfer by washing the resin with 90% TFA solution (3x4 ml) and add the washes to the conical vial. (Use your PTFE stir rod to return any beads adhering to the syringe wall back into the solution.)... 

Centrifuge the 50 ml conical vial containing your precipitated peptide for 5 min at... 

Trypsin-EDTA (lx) 0.25% trypsin, 1 mM Na4EDTA (Gibco)—upon arrival, this should be partitioned into 10 ml aliquots in 15 ml sterile conical vials. 

After you have passed your cells, remove 150 pi of cell suspension and add this to a 1 ml conical vial containing 150 pi of a-MEM. Flick the flask to thoroughly mix. 

Prepare flow hood (see Sterile Technique above) and supply one 15 ml conical vial (labeled CHO cells), approximately five 10 ml graduated pipettes, 1000 and 200 pi pipetmen and a waste beaker. 

Incubate in a 37°C water bath, the bottles of a-MEM (with 10% calf serum) and PBS and one 15 ml conical vial containing trypsin-EDTA solution. 

I ve put drawings of microscale equipment I ve had occasion to use in this section, along with some discussion ofthe 0-ring seals, conical vials, drying tubes, and so on. I ve put full descriptions of certain microscale apparatus with the operations they re used in. So Craig tubes show up with recrystallization the Hickman still is with distillation. 

In a famous microscale manual, you re told how to make one of these seals between an air condenser and a conical vial from the top down. In sum ... 

Get a conical vial (appropriate size) that ll fit on the bottom end (male joint) of the wide-bottom air condenser (Fig. 31). 

A 3 to 5 ml conical vial and T 14/10 joints. The plastic caps for these sizes have holes cut in them that are extremely tight. These... 

A 0.1 ml conical vial and f 515 joint. For these sizes, the hole in the cap is again snug around the male joint so that when you screw down the cap, the 0-ring doesn t squeeze out. This joint also appears to be very tight. 

The conical vial (Fig. 34) is the round-bottom flask of the microscale set, with considerably more hardware. When your glassware kit is brand new, every vial has a plastic cap, O-ring, and plastic disk to match. After one semester, every vial needs this stuff. When you check-in, make sure that every conical vial has a cap that fits and that you have at least one O-ring for each and a plastic disk that just fits inside this cap. 

Usually, you warm the conical vial somehow to have the reaction generate the gas you want to collect. 

If your capillary gas delivery tube was connected directly to a conical vial with an O-ring cap seal (Fig. 39a), you have to undo the seal on a hot vial ( )... 

Instead of a sep funnel, you use a conical vial and some Pasteur pipets. First you mix your extraction solvent with your product then you separate the two liquids. 

Get the material to be extracted into an appropriately sized conical vial. This vial should be at minimum twice the volume of the liquid you want to extract. Usually, this conical vial is the reaction vial for the experiment, so the choice is easily made (forced on you). 

Set up the stirring hot plate and sand bath. I suppose you could stick a thermometer in the sand to give you an idea of how warm the sand is. Put the sample you want to distill into an appropriately sized conical vial. Two things Don t fill the vial more than halfway, and don t use such a big vial that the product disappears. Less than one third full, and you ll lose a lot on the walls of the vial. 

Get a clamp ready to accept the Hickman still at about the position you want just open and loosely clamped—horizontally—to the ringstand. Scrunch the conical vial down into the sand, and push it to where you want it to be. Hold the vial at the top. 

The Conical Vial As Vial Packaging Oops Tare to the Analytical Balance Electronic Analytical Balance Heating These Vials... 

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